scholarly journals Collagen IV differentially regulates planarian stem cell potency and lineage progression

2021 ◽  
Vol 118 (16) ◽  
pp. e2021251118
Author(s):  
Andy Chan ◽  
Sophia Ma ◽  
Bret J. Pearson ◽  
Danny Chan
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Type Iv Collagen ◽  
Large Population ◽  
Ideal Model ◽  
Multipotent Stem Cells ◽  
Stem Cell Regulation ◽  
Type Iv ◽  
Cell Niche

The extracellular matrix (ECM) provides a precise physical and molecular environment for cell maintenance, self-renewal, and differentiation in the stem cell niche. However, the nature and organization of the ECM niche is not well understood. The adult freshwater planarian Schmidtea mediterranea maintains a large population of multipotent stem cells (neoblasts), presenting an ideal model to study the role of the ECM niche in stem cell regulation. Here we tested the function of 165 planarian homologs of ECM and ECM-related genes in neoblast regulation. We identified the collagen gene family as one with differential effects in promoting or suppressing proliferation of neoblasts. col4-1, encoding a type IV collagen α-chain, had the strongest effect. RNA interference (RNAi) of col4-1 impaired tissue maintenance and regeneration, causing tissue regression. Finally, we provide evidence for an interaction between type IV collagen, the discoidin domain receptor, and neuregulin-7 (NRG-7), which constitutes a mechanism to regulate the balance of symmetric and asymmetric division of neoblasts via the NRG-7/EGFR pathway.

scholarly journals Closer to Nature: The Role of MSCs in Recreating the Microenvironment of the Hematopoietic Stem Cell Niche in vitro

2022 ◽  
pp. 1-10
Author(s):  
Patrick Wuchter ◽  
Anke Diehlmann ◽  
Harald Klüter
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Model Systems ◽  
Bone Marrow Niche ◽  
Hematopoietic Stem ◽  
Hematopoietic Stem Cell Niche ◽  
Cell Niche

<b><i>Background:</i></b> The stem cell niche in human bone marrow provides scaffolds, cellular frameworks and essential soluble cues to support the stemness of hematopoietic stem and progenitor cells (HSPCs). To decipher this complex structure and the corresponding cellular interactions, a number of in vitro model systems have been developed. The cellular microenvironment is of key importance, and mesenchymal stromal cells (MSCs) represent one of the major cellular determinants of the niche. Regulation of the self-renewal and differentiation of HSPCs requires not only direct cellular contact and adhesion molecules, but also various cytokines and chemokines. The C-X-C chemokine receptor type 4/stromal cell-derived factor 1 axis plays a pivotal role in stem cell mobilization and homing. As we have learned in recent years, to realistically simulate the physiological in vivo situation, advanced model systems should be based on niche cells arranged in a three-dimensional (3D) structure. By providing a dynamic rather than static setup, microbioreactor systems offer a number of advantages. In addition, the role of low oxygen tension in the niche microenvironment and its impact on hematopoietic stem cells need to be taken into account and are discussed in this review. <b><i>Summary:</i></b> This review focuses on the role of MSCs as a part of the bone marrow niche, the interplay between MSCs and HSPCs and the most important regulatory factors that need to be considered when engineering artificial hematopoietic stem cell niche systems. <b><i>Conclusion:</i></b> Advanced 3D model systems using MSCs as niche cells and applying microbioreactor-based technology are capable of simulating the natural properties of the bone marrow niche more closely than ever before.

scholarly journals Maintenance of Adult Stem Cells: Role of the Stem Cell Niche

2011 ◽  
pp. 35-55 ◽  
Author(s):  
Yoshiko Matsumoto ◽  
Hiroko Iwasaki ◽  
Toshio Suda
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Adult Stem Cells ◽  
Cell Niche

scholarly journals The role of stem cell niche in intestinal aging

2020 ◽  
Vol 191 ◽  
pp. 111330
Author(s):  
Nalle Pentinmikko ◽  
Pekka Katajisto
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Cell Niche

scholarly journals Role of the stem cell niche in the pathogenesis of epithelial ovarian cancers

2014 ◽  
Vol 1 (3) ◽  
pp. e963435 ◽  
Author(s):  
Andrea Flesken-Nikitin ◽  
Ashley A Odai-Afotey ◽  
Alexander Yu Nikitin
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Ovarian Cancers ◽  
Cell Niche

scholarly journals Stem Cell Regulation by the Haemopoietic Stem Cell Niche

Cell Cycle ◽  
2005 ◽  
Vol 4 (10) ◽  
pp. 1353-1355 ◽  
Author(s):  
David N. Haylock ◽  
Susan K. Nilsson
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Cell Regulation ◽  
Stem Cell Regulation ◽  
Haemopoietic Stem Cell ◽  
Cell Niche

Role of Integrins in Adhesion of Hematopoietic Progenitor Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4263-4263
Author(s):  
Shawdee Eshghi ◽  
Jing Zhang ◽  
Linda G. Griffith ◽  
Harvey F. Lodish
Keyword(s):  
Stem Cell ◽  
Stem Cell Niche ◽  
Fetal Liver ◽  
Hematopoietic Stem ◽  
Cell Matrix ◽  
Hematopoietic Stem Cell Niche ◽  
Matrix Interactions ◽  
Cell Niche ◽  
Cell Matrix Interactions

Abstract The hematopoietic stem cell niche is the set of soluble growth factors, cell-cell and cell-matrix interactions that contribute to stem cell self renewal in the bone marrow. While cytokines and cell-cell interactions have been well documented, cell-matrix interactions in the niche are less understood. Integrins are a class of highly conserved cell adhesion molecules that are important in hematopoietic development and homing. However the specific role of integrins in mediating adhesion to extracellular matrix in the hematopoietic stem cell niche is unknown. The terminal stages of erythropoiesis in the fetal liver provide a good model system with which to develop several of the assays to be used with HSCs. Using flow cytometry, murine fetal liver erythroid progenitors can be separated at four distinct stages of development based on expression of CD71 and Ter119. Further FACS and quantitative PCR analysis revealed that α4β1 integrin is significantly downregulated over the course of erythroid differentiation. Using a centrifugation assay, we determined that this change is accompanied by a loss of adhesion to fibronectin, and that adhesion to fibronectin is blocked by addition of anti-integrin antibodies. Finally, fetal liver progenitor cells adhered to comb co-polymer surfaces engineered to present peptides specifically recognized by α4β1 integrins. By determining the integrin profile expressed by hematopoietic stem cells and measuring stem cell adhesion to ECM in a similar manner, we can begin to understand how these specific interactions present developmental cues important to maintaining the stem cell phenotype in vivo, in addition to leading to design parameters for ex vivo culture systems.

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