scholarly journals Cap analysis gene expression for high-throughput analysis of transcriptional starting point and identification of promoter usage

2003 ◽  
Vol 100 (26) ◽  
pp. 15776-15781 ◽  
Author(s):  
T. Shiraki ◽  
S. Kondo ◽  
S. Katayama ◽  
K. Waki ◽  
T. Kasukawa ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Throughput ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Starting Point ◽  
Promoter Usage ◽  
Cap Analysis

scholarly journals High-throughput analysis of candidate imprinted genes and allele-specific gene expression in the human term placenta

BMC Genetics ◽  
2010 ◽  
Vol 11 (1) ◽  
pp. 25 ◽  
Author(s):  
Caroline Daelemans ◽  
Matthew E Ritchie ◽  
Guillaume Smits ◽  
Sayeda Abu-Amero ◽  
Ian M Sudbery ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Throughput ◽  
Specific Gene ◽  
Imprinted Genes ◽  
High Throughput Analysis ◽  
Human Term Placenta ◽  
Throughput Analysis ◽  
Term Placenta ◽  
Specific Gene Expression ◽  
Allele Specific

scholarly journals High throughput analysis of TCR-β rearrangement and gene expression in single T cells

2006 ◽  
Vol 86 (3) ◽  
pp. 314-321 ◽  
Author(s):  
Dun Zhou ◽  
Rajneesh Srivastava ◽  
Verena Grummel ◽  
Sabine Cepok ◽  
Hans-Peter Hartung ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cells ◽  
High Throughput ◽  
High Throughput Analysis ◽  
Throughput Analysis

A high-density quantitative nuclease protection microarray platform for high throughput analysis of gene expression

2011 ◽  
Vol 154 (1) ◽  
pp. 68-75 ◽  
Author(s):  
K.M. Bourzac ◽  
M.P. Rounseville ◽  
X. Zarate ◽  
V.S.R.K. Maddula ◽  
D.C. Henderson ◽  
...  
Keyword(s):  
Gene Expression ◽  
High Throughput ◽  
Microarray Platform ◽  
High Density ◽  
High Throughput Analysis ◽  
Throughput Analysis

scholarly journals pBaSysBioII: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis

Microbiology ◽  
2010 ◽  
Vol 156 (6) ◽  
pp. 1600-1608 ◽  
Author(s):  
Eric Botella ◽  
Mark Fogg ◽  
Matthieu Jules ◽  
Sjouke Piersma ◽  
Geoff Doherty ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
High Throughput ◽  
Chromosomal Locus ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Ligation Independent Cloning ◽  
Phosphate Availability ◽  
Integrative Plasmid ◽  
Versatile Tool

Plasmid pBaSysBioII was constructed for high-throughput analysis of gene expression in Bacillus subtilis. It is an integrative plasmid with a ligation-independent cloning (LIC) site, allowing the generation of transcriptional gfpmut3 fusions with desired promoters. Integration is by a Campbell-type event and is non-mutagenic, placing the fusion at the homologous chromosomal locus. Using phoA, murAA, gapB, ptsG and cggR promoters that are responsive to phosphate availability, growth rate and carbon source, we show that detailed profiles of promoter activity can be established, with responses to changing conditions being measurable within 1 min of the stimulus. This makes pBaSysBioII a highly versatile tool for real-time gene expression analysis in growing cells of B. subtilis.

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