Studies on the recovery of crown gall tumor cells

Cultures of normal tobacco callus tissue 3 weeks after subculture contain free and bound forms of cinnamic, caffeic, and ferulic acids and bergapten, and bound umbelliferone, esculetin, and scopoletin. Cultures of tobacco crown gall tumor tissue lack cinnamic acid, umbelliferone, and bergapten; but they contain free as well as bound esculetin and scopoletin, and higher total levels of each of these coumarins than normal tissue. It is suggested that biosynthesis of coumarins in tumor cells has been diverted from the umbelliferone–furanocoumarin pathway in favor of esculetin and scopoletin.

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Quantitative Estimation of Agrobacterium tumefaciens DNA in Crown Gall Tumor Cells

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.71.9.3677 ◽

1974 ◽

Vol 71 (9) ◽

pp. 3677-3681 ◽

Cited By ~ 9

Author(s):

K. A. Drlica ◽

C. I. Kado

Keyword(s):

Agrobacterium Tumefaciens ◽

Tumor Cells ◽

Crown Gall ◽

Quantitative Estimation ◽

Crown Gall Tumor

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A Transformation-induced Alteration of Cellular Membranes in Crown Gall Tumor Cells

PLANT PHYSIOLOGY ◽

10.1104/pp.64.4.543 ◽

1979 ◽

Vol 64 (4) ◽

pp. 543-545 ◽

Cited By ~ 7

Author(s):

Larry E. Cockerham ◽

Carl V. Lundeen

Keyword(s):

Tumor Cells ◽

Crown Gall ◽

Cellular Membranes ◽

Crown Gall Tumor

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Starch-enhanced synthesis and release of amylolytic enzymes from normal and crown gall tumor tobacco tissue culture cells

Canadian Journal of Botany ◽

10.1139/b82-188 ◽

1982 ◽

Vol 60 (8) ◽

pp. 1474-1478 ◽

Cited By ~ 2

Author(s):

Charles Voliva ◽

Gustave W. Moessen ◽

Ann G. Matthysse

Keyword(s):

Tissue Culture ◽

Carbon Source ◽

Tumor Cells ◽

Crown Gall ◽

Amylolytic Activity ◽

Amylolytic Enzymes ◽

Crown Gall Tumor ◽

Culture Cells ◽

Tissue Culture Cells ◽

Tobacco Tissue Culture

The response of tobacco crown gall tumor cells growing in tissue culture to a substitution of starch for sucrose as the carbon source in the medium was compared with the response of normal tobacco tissue culture cells. In both cases amylolytic activity was secreted into the medium. The increase in extracellular amylolytic activity was preceded by an increase in intracellular activity. The increase in intracellular amylolytic enzymes was sensitive to cycloheximide and to actinomycin D indicating that enzyme induction at the level of mRNA synthesis was required. No significant difference between the responses of normal and tumor cells was observed. Thus crown gall tumor cells were as capable as normal cells of sensing and responding to an alteration in the carbon source in the external medium.

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