scholarly journals Induction of maturation (meiosis) in small Xenopus laevis oocytes by injection of maturation promoting factor.

1976 ◽  
Vol 73 (6) ◽  
pp. 2028-2032 ◽  
Author(s):  
J. Hanocq-Quertier ◽  
E. Baltus ◽  
J. Brachet
Keyword(s):  
Xenopus Laevis ◽  
Xenopus Laevis Oocytes ◽  
Maturation Promoting Factor

Progesterone-induced meiosis in xenopus laevis oocytes: a role for cAMP at the “maturation-promoting factor” level

Cell ◽  
1978 ◽  
Vol 15 (4) ◽  
pp. 1269-1275 ◽  
Author(s):  
Sabine Schorderet-Slatkine ◽  
Michel Schorderet ◽  
Patrice Boquet ◽  
François Godeau ◽  
Etienne-Emile Baulieu
Keyword(s):  
Xenopus Laevis ◽  
Xenopus Laevis Oocytes ◽  
Factor Level ◽  
Maturation Promoting Factor

scholarly journals A new system to evaluate characteristics of Niemann-Pick C1 Like 1-mediated cholesterol transport using Xenopus laevis oocytes

2021 ◽  
Vol 1863 (2) ◽  
pp. 183508
Author(s):  
Shunsuke Nashimoto ◽  
Saori Yagi ◽  
Naoki Takeda ◽  
Miku Nonaka ◽  
Yoh Takekuma ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Cholesterol Transport ◽  
Xenopus Laevis Oocytes ◽  
Niemann Pick ◽  
New System

scholarly journals Native cyclase-associated protein and actin from Xenopus laevis oocytes form a unique 4:4 complex with a tripartite structure

2021 ◽  
pp. 100649
Author(s):  
Noriyuki Kodera ◽  
Hiroshi Abe ◽  
Phuong Doan N. Nguyen ◽  
Shoichiro Ono
Keyword(s):  
Xenopus Laevis ◽  
Xenopus Laevis Oocytes

Characterization of Transport Activity of SLC11 Transporters in Xenopus laevis Oocytes by Fluorophore Quenching

2021 ◽  
pp. 247255522110041
Author(s):  
Raffaella Cinquetti ◽  
Francesca Guia Imperiali ◽  
Salvatore Bozzaro ◽  
Daniele Zanella ◽  
Francesca Vacca ◽  
...  
Keyword(s):  
Xenopus Laevis ◽  
Voltage Clamp ◽  
Expression System ◽  
Peptide Transporter ◽  
Xenopus Laevis Oocytes ◽  
Substrate Uptake ◽  
Transport Activity ◽  
Experimental Conditions ◽  
Metal Transporters ◽  
Electrode Voltage

Membrane proteins are involved in different physiological functions and are the target of pharmaceutical and abuse drugs. Xenopus laevis oocytes provide a powerful heterologous expression system for functional studies of these proteins. Typical experiments investigate transport using electrophysiology and radiolabeled uptake. A two-electrode voltage clamp is suitable only for electrogenic proteins, and uptake measurements require the existence of radiolabeled substrates and adequate laboratory facilities. Recently, Dictyostelium discoideum Nramp1 and NrampB were characterized using multidisciplinary approaches. NrampB showed no measurable electrogenic activity, and it was investigated in Xenopus oocytes by acquiring confocal images of the quenching of injected fluorophore calcein. This method is adequate to measure the variation in emitted fluorescence, and thus transporter activity indirectly, but requires long experimental procedures to collect statistically consistent data. Considering that optimal expression of heterologous proteins lasts for 48–72 h, a slow acquiring process requires the use of more than one batch of oocytes to complete the experiments. Here, a novel approach to measure substrate uptake is reported. Upon injection of a fluorophore, oocytes were incubated with the substrate and the transport activity measured, evaluating fluorescence quenching in a microplate reader. The technique permits the testing of tens of oocytes in different experimental conditions simultaneously, and thus the collection of significant statistical data for each batch, saving time and animals. The method was tested with different metal transporters (SLC11), DMT1, DdNramp1, and DdNrampB, and verified with the peptide transporter PepT1 (SLC15). Comparison with traditional methods (uptake, two-electrode voltage clamp) and with quenching images acquired by fluorescence microscopy confirmed its efficacy.

scholarly journals Expression of Type I Iodothyronine 5′-Deiodinase in Xenopus laevis Oocytes

1989 ◽  
Vol 264 (6) ◽  
pp. 3054-3056
Author(s):  
D L St Germain ◽  
C M Morganelli
Keyword(s):  
Xenopus Laevis ◽  
Xenopus Laevis Oocytes ◽  
Type I
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