Membrane modification differentially affects the binding of the lactogenic hormones human growth hormone and ovine prolactin.

SUMMARY Agar gel double diffusion and immunoelectrophoresis of human growth hormone (HGH), ovine prolactin, bovine growth hormone (BGH) and a tryptic digest of BGH (TBGH) were performed. Antisera to BGH, TBGH, HGH and ovine prolactin were used for the tests. Both BGH and TBGH were found to contain material that precipitated with antiserum to ovine prolactin. Immunological analysis of various fractions of BGH and TBGH separated by chromatography on DEAE-cellulose showed that BGH and TBGH were eluted before prolactin. No differences between BGH and TBGH in their immunological reactions were found. HGH did not cross-react with BGH, TBGH or ovine prolactin.

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Comparative immunoenzymatic localization of prolactin and growth hormone in human and rat pituitaries.

Journal of Histochemistry & Cytochemistry ◽

10.1177/24.9.61240 ◽

1976 ◽

Vol 24 (9) ◽

pp. 1012-1016 ◽

Cited By ~ 19

Author(s):

J Martin-Comin ◽

C Robyn

Keyword(s):

Growth Hormone ◽

Horseradish Peroxidase ◽

Human Growth Hormone ◽

Human Growth ◽

Immunohistochemical Method ◽

Prolactin Cells ◽

Rabbit Immunoglobulin ◽

Ovine Prolactin ◽

Circulating Levels

Twelve human and twelve rat pituitaries were stained by an immunohistochemical method using a rabbit anti-ovine prolactin serum, a rabbit anti-human growth hormone serum and a sheep anti-rabbit immunoglobulin serum conjugated with horseradish peroxidase. On the same pituitary section, growth hormone cells were stained brown by using 3-3'-diaminobenzidine as peroxidase substrate, and prolactin cells were stained purplish blue by using 4-chloro-1-naphtol. Growth hormone cells outnumbered prolactin cells, especially in human pituitaries where the proportion is at least 10:1. No cells containing both brown granules stained for growth hormone and blue granules stained for prolactin were found in any of the sections examined. In the fetal pituitaries, there was no apparent hypertrophy of the prolactin cells, although the circulating levels of the hromone are known to be as high in the fetus at term as in the mother and much higher than in nonpregnant women.

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GROWTH-PROMOTING AND LUTEOTROPHIC ACTIVITIES OF HUMAN GROWTH HORMONE

Journal of Endocrinology ◽

10.1677/joe.0.0330249 ◽

1965 ◽

Vol 33 (2) ◽

pp. 249-258 ◽

Cited By ~ 19

Author(s):

ANNE STOCKELL HARTREE ◽

NADA KOVAČIĆ ◽

MARJORIE THOMAS

Keyword(s):

Growth Hormone ◽

Quantitative Study ◽

Human Growth Hormone ◽

Biological Activities ◽

Human Growth ◽

Ion Exchangers ◽

Immunological Activity ◽

Growth Promoting ◽

Ovine Prolactin

SUMMARY Human growth hormone prepared by Raben's (1959) method was chromatographed on various ion exchangers and the resulting fractions were assayed for growth-promoting, luteotrophic and immunological activity. A critical quantitative study of the biological activities of the fractions showed that there were significant differences between their ratios of growth-promoting to luteotrophic potency. The luteotrophic activity of some of the preparations was slightly higher than that of the 2nd International Ovine Prolactin Standard.

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