scholarly journals Isolation and expression of the eclosion hormone gene from the tobacco hornworm, Manduca sexta.

1989 ◽  
Vol 86 (20) ◽  
pp. 8123-8127 ◽  
Author(s):  
F. M. Horodyski ◽  
L. M. Riddiford ◽  
J. W. Truman
1983 ◽  
Vol 29 (12) ◽  
pp. 895-900 ◽  
Author(s):  
James W. Truman ◽  
Dorothy B. Rountree ◽  
Shirley E. Reiss ◽  
Lawrence M. Schwartz

1987 ◽  
Vol 146 (2) ◽  
pp. 746-750 ◽  
Author(s):  
Hiroshi Kataoka ◽  
Ruth G. Troetschler ◽  
Steven J. Kramer ◽  
Blake J. Cesarin ◽  
David A. Schooley

FEBS Letters ◽  
1987 ◽  
Vol 219 (2) ◽  
pp. 415-418 ◽  
Author(s):  
Thomas Marti ◽  
Koji Takio ◽  
Kenneth A. Walsh ◽  
Gail Terzi ◽  
James W. Truman

1980 ◽  
Vol 88 (1) ◽  
pp. 327-338 ◽  
Author(s):  
JAMES W. TRUMAN ◽  
PAUL H. TAGHERT ◽  
STUART E. REYNOLDS

The time of pupal ecdysis in Manduca sexta can be accurately predicted by the appearance of external markers late in pupal development. Eclosion hormone injections resulted in premature ecdysis of staged pharate pupae. The latency between injection and initiation of ecdysis movements was inversely correlated with dosage and served as the basis for a sensitive, rapid, and quantitative bioassay for the hormone. Behavioural responsiveness to the hormone arose about 8 h before normal ecdysis at 23 °C. When insects were injected at these early times the larval cuticle was not sufficiently digested to allow its rupture and these animals remained permanently trapped inside the larval cuticle. Measurement of eclosion hormone in the blood of pharate pupae showed the appearance of biological activity in the blood about 30 min before the onset of ecdysis. It is concluded that pupal ecdysis is under endocrine control and that the hormone involved is likely to be the eclosion hormone.


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