scholarly journals Prediction of major histocompatibility complex binding regions of protein antigens by sequence pattern analysis.

1989 ◽  
Vol 86 (9) ◽  
pp. 3296-3300 ◽  
Author(s):  
A. Sette ◽  
S. Buus ◽  
E. Appella ◽  
J. A. Smith ◽  
R. Chesnut ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Pattern Analysis ◽  
Major Histocompatibility ◽  
Protein Antigens ◽  
Sequence Pattern ◽  
Major Histocompatibility Complex Binding ◽  
Histocompatibility Complex ◽  
Binding Regions

scholarly journals Defective major histocompatibility complex class II assembly, transport, peptide acquisition, and CD4+ T cell selection in mice lacking invariant chain expression.

1993 ◽  
Vol 177 (6) ◽  
pp. 1699-1712 ◽  
Author(s):  
E K Bikoff ◽  
L Y Huang ◽  
V Episkopou ◽  
J van Meerwijk ◽  
R N Germain ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Mhc Class Ii ◽  
Critical Role ◽  
Class Ii ◽  
Intact Protein ◽  
Invariant Chain ◽  
Major Histocompatibility ◽  
Protein Antigens ◽  
Histocompatibility Complex ◽  
Class Ii Alpha

We used gene targeting techniques to produce mice lacking the invariant chain associated with major histocompatibility complex (MHC) class II molecules. Cells from these mice show a dramatic reduction in surface class II, resulting from both defective association of class II alpha and beta chains and markedly decreased post-Golgi transport. The few class II alpha/beta heterodimers reaching the cell surface behave as if empty or occupied by an easily displaced peptide, and display a distinct structure. Mutant spleen cells are defective in their ability to present intact protein antigens, but stimulate enhanced responses in the presence of peptides. These mutant mice have greatly reduced numbers of thymic and peripheral CD4+ T cells. Overall, this striking phenotype establishes that the invariant chain plays a critical role in regulating MHC class II expression and function in the intact animal.

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