scholarly journals The consensus sequence of a major Alu subfamily contains a functional retinoic acid response element.

1995 ◽  
Vol 92 (18) ◽  
pp. 8229-8233 ◽  
Author(s):  
G. Vansant ◽  
W. F. Reynolds
Keyword(s):  
Retinoic Acid ◽  
Consensus Sequence ◽  
Response Element ◽  
Retinoic Acid Response Element

scholarly journals Identification of a retinoic acid response element upstream of the murine Hox-4.2 gene.

1993 ◽  
Vol 13 (1) ◽  
pp. 257-265 ◽  
Author(s):  
H Pöpperl ◽  
M S Featherstone
Keyword(s):  
Retinoic Acid ◽  
Hox Genes ◽  
Regulatory Element ◽  
Consensus Sequence ◽  
Regulatory Region ◽  
Dominant Negative ◽  
Luciferase Reporter ◽  
Response Element ◽  
Retinoic Acid Response Element ◽  
Ec Cells

Hox genes play an important role in the process of vertebrate pattern formation, and their expression is intricately regulated both temporally and spatially. All-trans-retinoic acid (RA), a physiologically active metabolite of vitamin A, affects the expression of a large number of Hox genes in vitro and in vivo. However, the regulatory mechanisms underlying the RA response of these genes have not been extensively studied, and no response element for RA receptors (RARs) has been characterized in a Hox regulatory region. The expression of murine Hox-4.2 and its human homolog, HOX4B, is increased in embryonal carcinoma (EC) cell lines upon RA treatment (M. S. Featherstone, A. Baron, S. J. Gaunt, M.-G. Mattei, and D. Duboule, Proc. Natl. Acad. Sci. USA 85:4760-4764, 1988; A. Simeone, D. Acampora, V. Nigro, A. Faiella, M. D'Esposito, A. Stornaiuolo, F. Mavilio, and E. Boncinelli, Mech. Dev. 33:215-228, 1991). Using transient expression assays, we showed that luciferase reporter gene constructs carrying genomic sequences located upstream of Hox-4.2 responded to RA in murine P19 EC cells. A 402-bp NcoI fragment was necessary for the RA responsiveness of reporter constructs. This fragment contained a regulatory element, 5'-AGGTGA(N)5AGGTCA-3', that closely resembles the consensus sequence for an RA response element. The Hox-4.2 RA response element was critical for the RA induction and specifically bound RARs. In addition, the response to RA could be inhibited by expressing a dominant negative form of RAR alpha in transfected P19 EC cells. These results suggested that Hox-4.2 is a target for RAR-mediated regulation by RA.

Identification of a retinoic acid response element upstream of the murine Hox-4.2 gene

1993 ◽  
Vol 13 (1) ◽  
pp. 257-265 ◽  
Author(s):  
H Pöpperl ◽  
M S Featherstone
Keyword(s):  
Retinoic Acid ◽  
Hox Genes ◽  
Regulatory Element ◽  
Consensus Sequence ◽  
Regulatory Region ◽  
Dominant Negative ◽  
Luciferase Reporter ◽  
Response Element ◽  
Retinoic Acid Response Element ◽  
Ec Cells

Hox genes play an important role in the process of vertebrate pattern formation, and their expression is intricately regulated both temporally and spatially. All-trans-retinoic acid (RA), a physiologically active metabolite of vitamin A, affects the expression of a large number of Hox genes in vitro and in vivo. However, the regulatory mechanisms underlying the RA response of these genes have not been extensively studied, and no response element for RA receptors (RARs) has been characterized in a Hox regulatory region. The expression of murine Hox-4.2 and its human homolog, HOX4B, is increased in embryonal carcinoma (EC) cell lines upon RA treatment (M. S. Featherstone, A. Baron, S. J. Gaunt, M.-G. Mattei, and D. Duboule, Proc. Natl. Acad. Sci. USA 85:4760-4764, 1988; A. Simeone, D. Acampora, V. Nigro, A. Faiella, M. D'Esposito, A. Stornaiuolo, F. Mavilio, and E. Boncinelli, Mech. Dev. 33:215-228, 1991). Using transient expression assays, we showed that luciferase reporter gene constructs carrying genomic sequences located upstream of Hox-4.2 responded to RA in murine P19 EC cells. A 402-bp NcoI fragment was necessary for the RA responsiveness of reporter constructs. This fragment contained a regulatory element, 5'-AGGTGA(N)5AGGTCA-3', that closely resembles the consensus sequence for an RA response element. The Hox-4.2 RA response element was critical for the RA induction and specifically bound RARs. In addition, the response to RA could be inhibited by expressing a dominant negative form of RAR alpha in transfected P19 EC cells. These results suggested that Hox-4.2 is a target for RAR-mediated regulation by RA.

scholarly journals Characterization of thrombomodulin expression in response to retinoic acid and identification of a retinoic acid response element in the human thrombomodulin gene

1994 ◽  
Vol 269 (24) ◽  
pp. 16925-16932
Author(s):  
W.A. Dittman ◽  
S.C. Nelson ◽  
P.K. Greer ◽  
E.T. Horton ◽  
M.L. Palomba ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Response Element ◽  
Retinoic Acid Response Element

Cloning and Characterization of the Murine PKC α Promoter: Identification of a Retinoic Acid Response Element

1999 ◽  
Vol 263 (1) ◽  
pp. 28-34 ◽  
Author(s):  
Dinakar S. Desai ◽  
Syu-ichi Hirai ◽  
William E. Karnes ◽  
Richard M. Niles ◽  
Shi-geo Ohno
Keyword(s):  
Retinoic Acid ◽  
Response Element ◽  
Retinoic Acid Response Element

Retinoic acid increases surfactant protein mRNA in fetal rat lung in culture

1996 ◽  
Vol 271 (5) ◽  
pp. L862-L868 ◽  
Author(s):  
C. W. Bogue ◽  
H. C. Jacobs ◽  
D. W. Dynia ◽  
C. M. Wilson ◽  
I. Gross
Keyword(s):  
Retinoic Acid ◽  
Dose Response ◽  
Consensus Sequence ◽  
Surfactant Protein ◽  
Rat Lung ◽  
Response Element ◽  
Response Characteristics ◽  
Receptor Complexes ◽  
Fetal Rat ◽  
Fetal Rat Lung

Retinoic acid has both early or immediate (within hours) and late (after days) effects on gene expression. We studied the early effects of retinoic acid on the surfactant protein (SP) genes. Exposure of fetal rat lung explants to all trans-retinoic acid for 4 h resulted in a significant dose-dependent increase in SP-A, -B, and -C mRNA with markedly different dose-response characteristics. The maximal (2.5x) increase in SP-A mRNA was observed with 10(-10) M retinoic acid, whereas treatment with 10(-5) M resulted in a tendency to decreased levels. In contrast, maximal stimulation of SP-C (6x) was noted at 10(-5) M retinoic acid and that of SP-B (2x) at 10(-7) to 10(-5) M retinoic acid. Similar differences in the dose-response characteristics of SP-A and SP-C were observed with 9-cis-retinoic acid. A retinoic acid response element consensus sequence was identified in the rat SP-A gene; we hypothesize that retinoic acid-receptor complexes act directly on the SP-A gene via this response element.

scholarly journals Liganded retinoic acid X receptor α represses connexin 43 through a potential retinoic acid response element in the promoter region

2016 ◽  
Vol 80 (1) ◽  
pp. 159-168 ◽  
Author(s):  
Ruoyi Gu ◽  
Jun Xu ◽  
Yixiang Lin ◽  
Jing Zhang ◽  
Huijun Wang ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Promoter Region ◽  
Connexin 43 ◽  
Response Element ◽  
Retinoic Acid Response Element
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