scholarly journals Binding of the cellulose-binding domain of exoglucanase Cex from Cellulomonas fimi to insoluble microcrystalline cellulose is entropically driven.

1996 ◽  
Vol 93 (22) ◽  
pp. 12229-12234 ◽  
Author(s):  
A. L. Creagh ◽  
E. Ong ◽  
E. Jervis ◽  
D. G. Kilburn ◽  
C. A. Haynes
Keyword(s):  
Microcrystalline Cellulose ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Cellulomonas Fimi ◽  
Cellulose Binding ◽  
Entropically Driven

scholarly journals Enzyme Immobilization Using the Cellulose-Binding Domain of a Cellulomonas Fimi Exoglucanase

1989 ◽  
Vol 7 (6) ◽  
pp. 604-607 ◽  
Author(s):  
Edgar Ong ◽  
Neil R. Gilkes ◽  
R. Antony J. Warren ◽  
Robert C. Miller ◽  
Douglas G. Kilburn
Keyword(s):  
Enzyme Immobilization ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Cellulomonas Fimi ◽  
Cellulose Binding

The cellulose-binding domain of endoglucanase A (CenA) from Cellulomonas fimi: evidence for the involvement of tryptophan residues in binding

1994 ◽  
Vol 11 (4) ◽  
pp. 747-755 ◽  
Author(s):  
Neena Din ◽  
Ian J. Forsythe ◽  
Leslie D. Burtnick ◽  
Neil R. Gilkes ◽  
Robert C. Miller ◽  
...  
Keyword(s):  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Cellulomonas Fimi ◽  
Tryptophan Residues ◽  
Cellulose Binding

Enzyme-linked assay of cellulose-binding domain functions from Cellulomonas fimi on multi-well microtiter plate

2013 ◽  
Vol 18 (3) ◽  
pp. 575-580 ◽  
Author(s):  
Hyeon-Dong Kim ◽  
Su-Lim Choi ◽  
Haseong Kim ◽  
Jung Hoon Sohn ◽  
Seung-Goo Lee
Keyword(s):  
Microtiter Plate ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Cellulomonas Fimi ◽  
Cellulose Binding

Trichoderma reesei cellobiohydrolase I with an endoglucanase cellulose-binding domain: action on bacterial microcrystalline cellulose

1997 ◽  
Vol 57 (1-3) ◽  
pp. 49-57 ◽  
Author(s):  
Malee Srisodsuk ◽  
Janne Lehtiö ◽  
Markus Linder ◽  
Emilio Margolles-clark ◽  
Tapani Reinikainen ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Microcrystalline Cellulose ◽  
Binding Domain ◽  
Cellulose Binding Domain ◽  
Cellobiohydrolase I ◽  
Cellulose Binding

scholarly journals Cellulose as an inert matrix for presenting cytokines to target cells: production and properties of a stem cell factor–cellulose-binding domain fusion protein

1999 ◽  
Vol 339 (2) ◽  
pp. 429-434
Author(s):  
J. Greg DOHENY ◽  
Eric J. JERVIS ◽  
M. Marta GUARNA ◽  
R. Keith HUMPHRIES ◽  
R. Antony J. WARREN ◽  
...  
Keyword(s):  
Stem Cell ◽  
Fusion Protein ◽  
Stem Cell Factor ◽  
Direct Analysis ◽  
Binding Domain ◽  
Target Cells ◽  
Cellulose Binding Domain ◽  
Binding Characteristics ◽  
Cellulose Binding ◽  
Cellulose Surface

A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine properties of SCF and the cellulose-binding characteristics of CBDCex. When adsorbed on cellulose, SCF–CBDCex is up to 7-fold more potent than soluble SCF–CBDCex and than native SCF at stimulating the proliferation of factor-dependent cell lines. When cells are incubated with cellulose-bound SCF–CBDCex, activated receptors and SCF–CBDCex co-localize on the cellulose matrix. The strong binding of SCF–CBDCex to the cellulose surface permits the effective and localized stimulation of target cells; this is potentially significant for long-term perfusion culturing of factor-dependent cells. It also permits the direct analysis of the effects of surface-bound cytokines on target cells.

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