scholarly journals Identification of Positive and Negative Determinants of Malonyl-CoA Sensitivity and Carnitine Affinity within the Amino Termini of Rat Liver- and Muscle-type Carnitine Palmitoyltransferase I

2000 ◽  
Vol 275 (49) ◽  
pp. 38410-38416 ◽  
Author(s):  
Vicky N. Jackson ◽  
Victor A. Zammit ◽  
Nigel T. Price
Keyword(s):  
Rat Liver ◽  
Carnitine Palmitoyltransferase ◽  
Muscle Type ◽  
Malonyl Coa ◽  
Carnitine Palmitoyltransferase I

scholarly journals Identification of Conserved Amino Acid Residues in Rat Liver Carnitine Palmitoyltransferase I Critical for Malonyl-CoA Inhibition

2002 ◽  
Vol 278 (11) ◽  
pp. 9058-9063 ◽  
Author(s):  
Montserrat Morillas ◽  
Paulino Gómez-Puertas ◽  
Assia Bentebibel ◽  
Eva Sellés ◽  
Nuria Casals ◽  
...  
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Carnitine Palmitoyltransferase ◽  
Amino Acid Residues ◽  
Malonyl Coa ◽  
Carnitine Palmitoyltransferase I

scholarly journals The Extreme C Terminus of Rat Liver Carnitine Palmitoyltransferase I Is Not Involved in Malonyl-CoA Sensitivity but in Initial Protein Folding

2002 ◽  
Vol 277 (49) ◽  
pp. 47184-47189 ◽  
Author(s):  
Yong Pan ◽  
Isabelle Cohen ◽  
Fanny Guillerault ◽  
Bruno Fève ◽  
Jean Girard ◽  
...  
Keyword(s):  
Protein Folding ◽  
Rat Liver ◽  
Carnitine Palmitoyltransferase ◽  
C Terminus ◽  
Malonyl Coa ◽  
Carnitine Palmitoyltransferase I

scholarly journals Reversible sensitization and desensitization of carnitine palmitoyltransferase I to inhibition by malonyl-CoA in isolated rat liver mitochondria. Significance for the mechanism of malonyl-CoA-induced sensitization

1983 ◽  
Vol 214 (3) ◽  
pp. 1027-1030 ◽  
Author(s):  
V A Zammit
Keyword(s):  
Rat Liver ◽  
Reduced Glutathione ◽  
Thiol Group ◽  
Liver Mitochondria ◽  
Carnitine Palmitoyltransferase ◽  
Rat Liver Mitochondria ◽  
Nitrobenzoic Acid ◽  
Malonyl Coa ◽  
Carnitine Palmitoyltransferase I ◽  
Cpt I

Preincubation of rat liver mitochondria with 5,5′-dithiobis-(2-nitrobenzoic acid) (Nbs2) followed by removal of excess reagent by washing the mitochondria with 0.5 mM-reduced glutathione resulted in a desensitization of carnitine palmitoyltransferase (CPT) I activity to malonyl-CoA inhibition. The effect was not observed if mitochondria were washed with 0.5 mM-dithiothreitol. The desensitization effect of Nbs2 could be reversed by a second incubation in the presence of 8 microM-malonyl-CoA. In addition, malonyl-CoA, when present simultaneously with Nbs2, protected CPT I activity against the desensitization effect of the thiol-group reagent. These results suggest that malonyl-CoA exerts an effect on one or more thiol groups of the enzyme, and that this effect is related to the ability of the metabolite to sensitize CPT I to malonyl-CoA inhibition.

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