Comparison between fluorescent lamps and light-emitting diodes on iin vitro/i growth of potato microplants and subsequent iin vivo/i performance and minituber production.

Author(s):  
O. H. Kwigizile ◽  
J. P. Palohuta ◽  
R. Kakuhenzire
HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 375-379 ◽  
Author(s):  
Ruey-Chi Jao ◽  
Wei Fang

Effects of intermittent light on photomixotrophic growth of potato plantlets in vitro and the electrical savings that could be realized by adjusting the frequency and duty ratio of light-emitting diodes (LEDs) were investigated and compared to the use of conventional tubular fluorescent lamps (TFLs). TFLs provide continuous fluctuating light at 60 Hz and LEDs provide continuous nonfluctuating or intermittent/pulse light depend on the preset frequency and duty ratio. In total, eight treatments were investigated with varying light source, frequency, duty ratio and photoperiod. Results indicated that if growth rate is the only concern, LEDs at 720 Hz [1.4 milliseconds (ms)] and 50% duty ratio with 16-h light/8-h dark photoperiod stimulated plant growth the most. However, if energy consumption is the major concern, using LEDs at 180 Hz (5.5 ms) and 50% duty ratio with 16-h light/8-h dark photoperiod would be the best choice for illuminating potato plantlets without significantly sacrificing plant growth, especially when energy for heat removal is also considered.


2017 ◽  
Vol 44 (7) ◽  
pp. 727 ◽  
Author(s):  
Karin Köhl ◽  
Takayuki Tohge ◽  
Mark Aurel Schöttler

For precise phenotyping, Arabidopsis thaliana (L.) Heynh. is grown under controlled conditions with fluorescent lamps as the predominant light source. Replacement by systems based on light emitting diodes (LED) could improve energy efficiency and stability of light quality and intensity. To determine whether this affects the reproducibility of results obtained under fluorescent lamps, four Arabidopsis accessions and a phytochrome mutant were grown and phenotyped under two different LED types or under fluorescent lamps. All genotypes had significantly higher rosette weight and seed mass and developed faster under LED light than under fluorescent lamps. However, differences between genotypes were reproducible independent of the light source. Chlorophyll content, photosynthetic complex accumulation and light response curves of chlorophyll fluorescence parameters were indistinguishable under LED and fluorescent light. Principal component analysis of leaf metabolite concentrations revealed that the effect of a change from fluorescent light to LED light was small compared with the diurnal effect, which explains 74% of the variance and the age effect during vegetative growth (12%). Altogether, the replacement of fluorescent lamps by LED allowed Arabidopsis cultivation and reproduction of results obtained under fluorescent light.


PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0247426
Author(s):  
Akira Okamoto ◽  
Miyuki Imamura ◽  
Kazune Tani ◽  
Takeru Matsumoto

Aquatic organisms have been used to investigate the safety of chemicals worldwide. One such assessment is an algal growth inhibition test. Algal growth inhibition tests are commonly performed using a growth chamber with fluorescent lamps as the lighting source, as test guidelines require continuous uniform fluorescent illumination. However, fluorescent lamps contain mercury, which has been identified as hazardous to humans and other organisms. The Minamata Convention (adopted in 2013) requires reduction or prohibition of products containing mercury. On the other hand, light-emitting diodes do not contain mercury and provide a photosynthetically effective wavelength range of 400–700 nm which is an adequate light intensity for algal growth. Light-emitting diodes are thus preferable to fluorescent lamps as a potential light source in algal growth inhibition tests. In this study, we investigated if light-emitting diodes could be substituted for fluorescent lamps in growth inhibition studies with green alga (Pseudokirchneriella subcapitata), diatom (Navicula pelliculosa), and cyanobacteria (Anabaena flos-aquae). Algal growth inhibition tests were performed using five different chemicals known to have different modes of action and are assigned as reference substances in the test guidelines. The results of each algal test showed similar values between light-emitting diodes and fluorescent lamps in terms of conditions for the growth inhibition rate and percent inhibition in yield of each chemical. It was therefore concluded that using light-emitting diodes instead of fluorescent lamps as a lighting source had no effect on the algal growth inhibition test results.


2014 ◽  
Vol 4 ◽  
pp. 384-387
Author(s):  
Akihiro Toyoda ◽  
Hiroshi Matsumura ◽  
Kazuyoshi Masumoto ◽  
Shun Sekimoto ◽  
Hiroshi Yashima ◽  
...  

HortScience ◽  
2009 ◽  
Vol 44 (1) ◽  
pp. 79-82 ◽  
Author(s):  
Gary W. Stutte ◽  
Sharon Edney ◽  
Tony Skerritt

Lactuca sativa cv. Outredgeous was grown under either fluorescent lamps or light-emitting diodes (LEDs) to test the hypothesis that antioxidant potential could be regulated by light quality. Red leaf lettuce was grown at 300 μmol·m−2·s−1 of photosynthetically active radiation, 1200 μmol·mol−1 CO2, 23 °C, and an 18 h-light /6-h dark photoperiod in controlled-environment chambers. The LED treatments were selected to provide different amounts of red (640 nm), blue (440 nm), green (530 nm), and far-red (730 nm) light in the spectra. Total anthocyanin content and the oxygen radical absorbance capacity of the tissue were measured at harvest. The source of light had a dramatic effect on both plant growth and production of radioprotective compounds. LEDs resulted in 50% greater bioprotectant content per plant at the same light level over triphosphor fluorescent lamps. Blue LEDs (440 nm) appeared to regulate the metabolic pathways leading to increased concentration of bioprotective compounds in leaf tissue. LED lighting induced a number of effects on morphology that increased both accumulation of bioprotective compounds and total yield.


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