G0 Potato Seed Production Management in Indonesia: An Overview and The Challenges

The availability of Indonesian potato seed and production of potato for consumption depends on the availability and quality of Generation 0 (G0) potato seed. This study aims to observe G0 potato seed production management in Indonesia. Data were collected through Focus Group Discussion (FGD) and survey. Thirty-seven G0 potato seed producers participated in the survey. Data were analyzed descriptively, while the feasibility of the farm budget of G0 potato production was calculated using the enterprise budget method. The results showed that the producers had challenges on capital to broaden the screen house to extend the production scale and limited plantlet availability. Meanwhile, they did not have any problems with other production aspects, such as controlling pests and diseases and the marketing of the G0 seed. Government supports to provide screen houses to fulfil producers' necessity and delegate the legality of potato plantlets are needed to create a better Indonesia's potato seed system.

In vitro potato plantlet development under different polychromatic LED spectra and dynamic illumination

We studied growth of potato plantlets of two cultivars under different constant polychromatic light-emitting diodes (LEDs) and dynamic lighting with the spectrum modifications during growth. Light sources constructed by us were sunbox SB, red-green-blue RGB, and full spectrum FS, red-blue. White luminescent lamps were used as control light. Dynamic lighting was achieved by transferring groups of plantlets from one box to another. Red/green/blue portions in the light sources were (in percents): 39R/39G/22B for SB, 63R/21G/16B for RGB, 74R/8G/18B for FS, and 22R/49G/29B for control. PPFD was set at 45 μmol/m2 s. Morphometric measurements were made 14 and 28 days after planting. Plantlets developed differently from initial stages. Cultivar 'Red Scarlett' appeared to be more susceptible to different lighting than 'Innovator'. Light variations RGB and RGB-SB were the most favorable for plantlet development. This is the first report on the dynamic lighting application for growing potato plantlets.

Effects of Yellow, Green, and Different Blue Spectra on Growth of Potato Plantlets In Vitro

The objectives of this study were to determine the effects of yellow light (Y), green light (G), and two blue lights (B) at different wavelengths in conjunction with red light (R) on the growth and morphogenesis of potato plantlets in vitro. Randomized nodal explants were cut into 1.0–1.5 cm pieces and were grown under five different light conditions: fluorescent white light (FL); the combined spectra of R, Y, and B at 445 nm (R630B445Y); the combined spectra of R, G, and B at 445 nm (R630B445G); the combined spectra of R, Y, and B at 465 nm (R630B465Y); and the combined spectra of R, G, and B at 465 nm (R630B465G). Morphogenesis and physiological parameters were investigated. The results showed that R630B445Y and R630B465Y increased the fresh weight (FW), dry weight (DW), stem diameter, blade number, leaf area, specific leaf weight (SLW), and the health index of potato plantlets in vitro; root activity increased significantly; and soluble sugar, soluble protein, and starch also increased. The addition of Y to the combined spectra of R and B contributed to the growth, development, and morphogenesis more than the combined spectra of R and B with G, and B at 445 nm was more effective at promoting plant growth than was B at 465 nm.