Factors influencing shoot regeneration from cotyledons of tetraploid Isatis indigotica fort

2003 ◽  
Vol 39 (5) ◽  
pp. 459-462 ◽  
Author(s):  
Lei Zhang ◽  
Tiefeng Xu ◽  
Xiaofen Sun ◽  
Hanming Zhang ◽  
Tianlin Tang ◽  
...  

1989 ◽  
Vol 18 (3) ◽  
pp. 313-319 ◽  
Author(s):  
Randall P. Niedz ◽  
Sandra Schiller Smith ◽  
Kerry B. Dunbar ◽  
Christine T. Stephens ◽  
Harry H. Murakishi


2010 ◽  
Vol 101 (1) ◽  
pp. 111-117 ◽  
Author(s):  
Jian-Can Feng ◽  
X. M. Yu ◽  
X. L. Shang ◽  
J. D. Li ◽  
Y. X. Wu


2013 ◽  
Vol 49 (5) ◽  
pp. 529-540 ◽  
Author(s):  
Chao Zhang ◽  
Shaoping Fu ◽  
Gaijuan Tang ◽  
Xinwen Hu ◽  
Jianchun Guo


2003 ◽  
Vol 189 (5) ◽  
pp. 351-358 ◽  
Author(s):  
G. X. Tang ◽  
W. J. Zhou ◽  
H. Z. Li ◽  
B. Z. Mao ◽  
Z. H. He ◽  
...  


2015 ◽  
Vol 42 (4) ◽  
pp. 370-375
Author(s):  
Hyun Suk Lee ◽  
Hyun Rho Park ◽  
Hyun seak Kim ◽  
Chang Kil Kim


2017 ◽  
Vol 29 (6) ◽  
pp. 1533-1545 ◽  
Author(s):  
Yan Zhang ◽  
Beibei Wang ◽  
Liqin Guo ◽  
Wenting Xu ◽  
Zewei Wang ◽  
...  


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 442B-442
Author(s):  
Qingzhong Liu ◽  
Sarbagh Salih ◽  
Freddi Hammerschlag

Factors influencing regeneration and ß-glucuronidase expression from apple (Malus × domestica Borkh.) stem internodes were studied as part of a program to develop transgenic `Royal Gala' apple with improved disease resistance. The early stages of the transformation process were monitored by counting the number of ß-glucuronidase (GUS) expressing zones immediately after co-cultivation of explants with Agrobacterium tumefaciens supervirulent strain EHA105 (p35SGUS_INT) and by counting the number of GUS-expressing calli developing on explants 2 weeks after co-cultivation. Etiolated shoots were produced from in vitro shoots cultured for 2 weeks in the light followed by 2 weeks in the dark and were compared with shoots cultured for 4 weeks in the light (green shoots). First internodes from etiolated shoots produced three, 10 and 100 times the number of shoots regenerated from second, third, and fourth internodal explants, respectively, and produced seven times the number of shoots compared with similar explants from green shoots. 100% of first internodes from etiolated shoots exhibited GUS-expressing zones and yielded twice as many GUS-expressing zones when compared with leaf explants from green shoots, which exhibited GUS-expressing zones in only 60% of the explants. An average of nine GUS-expressing calli per explant were produced on first internodes from etiolated shoots 2 weeks after co-cultivation.



2014 ◽  
Vol 337 (6) ◽  
pp. 383-390 ◽  
Author(s):  
Aung Htay Naing ◽  
Su Min Jeon ◽  
Jeung-Sul Han ◽  
Sun Hyung Lim ◽  
Ki Byung Lim ◽  
...  


Author(s):  
Julie A. Martini ◽  
Robert H. Doremus

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.



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