Factors influencing adhesive bonding at the bone/implant interface

Author(s):  
Julie A. Martini ◽  
Robert H. Doremus

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.

1990 ◽  
Vol 36 (2) ◽  
pp. 140-144 ◽  
Author(s):  
Franoise Rieu ◽  
G. Fonty ◽  
Brigitte Gaillard ◽  
P. Gouet

The lamb rumen walls were rapidly colonized by an abundant bacterial population after birth. This colonization was examined by electron microscopy in neonatal conventional lambs. The sequence of establishment of the epimural species during the 3 weeks following birth, and the distribution of bacteria on the different sacs of the rumen, were examined by scanning electron microscopy. The population was very dense and consisted of a limited number of morphological types by 2 days after birth. Three types of rods were dominant at that time. The microflora was more complex 2 weeks later. Observations by transmission electron microscopy of desquamated epithelial cells revealed the presence of adherent bacteria that are surrounded by fibrous carbohydrate coats and sometimes partially enclosed by invaginations of the epithelial cell. Key words: rumen, lamb, microflora, scanning electron microscopy, adherence.


1987 ◽  
Vol 111 ◽  
Author(s):  
Roseann Csencsits ◽  
Ronald Gronsky ◽  
Vinayan Nair ◽  
Rosemarie Szostak

AbstractThe effects of various synthesis conditions on the structure and composition of ferrisilicate analogs of zeolite ZSM-5 were considered. Scanning electron microscopy (SEM) was used to determine the particles size distributions and morphologies. Particle sizes vary from tenths of a micron to several microns, depending on degree of agitation during crystal growth, while morphology is additionally dependent on the concentration of iron in the gel during crystallization.X-ray emissive spectroscopy (XES) performed in the transmission electron microscope (TEM) was used to determine their composition variation. The distribution of iron amongst the crystals is more homogeneous if the gel is stirred and it does not depend on particle size.


1987 ◽  
Vol 21 (1) ◽  
pp. 48-52 ◽  
Author(s):  
J. P. Koopman ◽  
A. M. Stadhouders ◽  
H. M. Kennis ◽  
H. De Boer

Scanning electron micrographs are presented of the ileal epithelium of mice aged 5, 15, 20 and 25 days. During this period the villous pattern develops to full maturity. By the twentieth day of life a segmented filamentous micro-organism colonizes the ileal epithelium and is firmly attached via a small segment. During the first days of colonization the segmented filamentous micro-organisms themselves are subcolonized by small rod-shaped bacteria, presumably lactobacilli. At the age of 25 days this subcolonization was no longer observed.


Sign in / Sign up

Export Citation Format

Share Document