Plant regeneration, origin, and development of shoot buds from root segments of Melia azedarach L. (Meliaceae) seedlings

2005 ◽  
Vol 41 (6) ◽  
pp. 746-751 ◽  
Author(s):  
Silvia Vila ◽  
Ana Gonzalez ◽  
Hebe Rey ◽  
Luis Mroginski
HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 569-571 ◽  
Author(s):  
A. Raymond Miller ◽  
Craig K. Chandler

A protocol was developed for excising and culturing cotyledon explants from mature achenes of strawberry (Fragaria × ananassa Duch.). Cotyledon explants formed callus with multiple shoot buds on agar-solidified Murashige and Skoog media containing several combinations of hormones (1 μm 2,4-D; 10 μm 2,4-D; 1 μm BA + 1 μm 2,4-D; 1 μm BA + 10 μm 2,4-D; 5 μm BA; 5 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μ m 2,4-D; 5 μ m BA + 5 μm NAA; 5 μ m BA + 15 μ m NAA). After three subcultures, only tissues maintained on the medium containing 5 μm BA + 5 μm NAA continued to form shoots. Tissues transferred to other media eventually died (1 μm 2,4-D; 1 μ m BA + 10 μ m 2,4-D; 5 μ m BA; 5 μ m BA + 1 μ m 2,4-D), became unorganized (1 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μm 2,4-D; 5 μm BA + 15 μm NAA), or formed roots (10 μm 2,4-D). Whole plantlets were produced by transferring callus with buds to medium lacking hormones. The rapid regeneration of clonal plantlets from cotyledon explants may be useful for reducing variability in future developmental studies. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); (2,4-dichlorophenoxy) acetic acid (2,4-D); and 1-naphthaleneacetic acid (NAA).


1998 ◽  
Vol 18 (1-2) ◽  
pp. 127-131 ◽  
Author(s):  
R. Thakur ◽  
P. S. Rao ◽  
V. A. Bapat

2009 ◽  
Vol 3 (3) ◽  
pp. 175-182 ◽  
Author(s):  
Rakesh Yadav ◽  
Pooja Arora ◽  
Dharmendar Kumar ◽  
Dinesh Katyal ◽  
Neeraj Dilbaghi ◽  
...  

2007 ◽  
Vol 34 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Su-Jin Jung ◽  
Jae-Hun Jeong ◽  
Eui-Soo Yoon ◽  
Yong-Eui Choi

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