Distributional characteristics of sulfated glycosaminoglycans in normal human nasal mucosa and nasal polyp

Surfactant proteins (SPs), designated SP-A, SP-B, SP-C, and SP-D, play an important role in surfactant metabolism and host defense mechanisms in the lung. This study investigates expression of the different SP types in human nasal mucosa and cultured normal human nasal epithelial (NHNE) cells and whether the expression of SP mRNA is influenced by the degree of mucociliary differentiation. RT-PCR was performed with mRNA from cultured NHNE cells and nasal mucosa. Immunohistochemical staining for SPs was performed on nasal mucosa specimens. Western blot analysis was performed on cell lysates from cultured NHNE cells. SP-A2, SP-B, and SP-D mRNAs were expressed in normal NHNE cells and human nasal mucosa. SPs were localized in ciliated cells of the surface epithelium and serous acini of the submucosal glands. SP-A, SP-B, and SP-D proteins were expressed in cultured NHNE cells. The degree of mucociliary differentiation influenced expression of the SP gene. We demonstrate that SP-A, SP-B, and SP-D are expressed in human nasal mucosa and cultured NHNE cells. Further study of the functional role of SPs in the upper airway is required.

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Expression and distribution of thioredoxin and thioredoxin reductase in human nasal mucosa and nasal polyp

Acta Oto-Laryngologica ◽

10.1080/00016480510029293 ◽

2005 ◽

Vol 125 (8) ◽

pp. 877-882 ◽

Cited By ~ 8

Author(s):

Seung Hoon Lee ◽

Woong Sik Kim ◽

Sang Hag Lee ◽

Joon Whan Oh ◽

Heung Man Lee ◽

...

Keyword(s):

Nasal Mucosa ◽

Nasal Polyp ◽

Thioredoxin Reductase ◽

Human Nasal Mucosa

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Expression and distribution patterns of the stem cell marker, nestin, and the stem cell renewal factor, BMI-1, in normal human nasal mucosa and nasal polyps

Acta Oto-Laryngologica ◽

10.1080/00016480802527560 ◽

2009 ◽

Vol 129 (9) ◽

pp. 996-1001 ◽

Cited By ~ 4

Author(s):

Tae Hoon Kim ◽

Heung Man Lee ◽

Seung Hoon Lee ◽

Hwan Choe ◽

Ha Kyun Kim ◽

...

Keyword(s):

Stem Cell ◽

Nasal Mucosa ◽

Nasal Polyps ◽

Distribution Patterns ◽

Stem Cell Marker ◽

Cell Renewal ◽

Cell Marker ◽

Human Nasal Mucosa ◽

Stem Cell Renewal ◽

Normal Human

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Immunolocalisation of heme oxygenase isoforms in human nasal polyps

The Journal of Laryngology & Otology ◽

10.1017/s0022215107001284 ◽

2008 ◽

Vol 123 (1) ◽

pp. 131-135 ◽

Cited By ~ 2

Author(s):

S Lo ◽

S Di Palma ◽

E George ◽

A W McCombe

Keyword(s):

Heme Oxygenase ◽

Nasal Mucosa ◽

Nasal Polyp ◽

Nasal Polyps ◽

Nasal Polyposis ◽

Inflammatory Diseases ◽

Immunohistochemical Analysis ◽

Respiratory Epithelium ◽

Human Nasal Mucosa ◽

Pathophysiological Role

AbstractBackground:Carbon monoxide is an endogenous vasodilator gas produced by the enzyme heme oxygenase (HO). HO is expressed in human nasal mucosa, but its pathophysiological role in nasal inflammatory diseases is not fully understood. The aim of this study was to detect and compare the expression of HO-1 and -2 isoforms in nasal polyps with normal nasal mucosa.Methods:Immunohistochemical analysis using antibodies specific for HO-1 and -2 was conducted on nasal polyps from nine patients with allergic nasal polyposis, and on normal nasal mucosa from six controls.Results:Intense HO-1 immunoreactivity was observed in nasal polyp epithelium but was absent in normal nasal mucosa. HO-2 staining was observed in respiratory epithelium, vascular endothelium and seromucous glands, with no difference observed between nasal polyps and normal nasal mucosa.Conclusions:HO-1 expression is up-regulated in nasal polyp epithelium, supporting the theory that respiratory epithelium plays a role in the pathogenesis of nasal polyposis.

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