Enhancement of Symbiotic Nitrogen Fixation Activity ofBradyrhizobium japonicumby Acridine Orange

Little is known about the genes participating in digalactosyldiacylglycerol (DGDG) synthesis during nodule symbiosis. Here, we identified full-length MtDGD1, a synthase of DGDG, and characterized its effect on symbiotic nitrogen fixation in Medicago truncatula. Immunofluorescence and immunoelectron microscopy showed that MtDGD1 was located on the symbiosome membranes in the infected cells. β-Glucuronidase histochemical staining revealed that MtDGD1 was highly expressed in the infection zone of young nodules as well as in the whole mature nodules. Compared with the control, MtDGD1-RNA interference transgenic plants exhibited significant decreases in nodule number, symbiotic nitrogen fixation activity, and DGDG abundance in the nodules, as well as abnormal nodule and symbiosome development. Overexpression of MtDGD1 resulted in enhancement of nodule number and nitrogen fixation activity. In response to phosphorus starvation, the MtDGD1 expression level was substantially upregulated and the abundance of nonphospholipid DGDG was significantly increased in the roots and nodules, accompanied by corresponding decreases in the abundance of phospholipids such as phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol. Overall, our results indicate that DGD1 contributes to effective nodule organogenesis and nitrogen fixation by affecting the synthesis and content of DGDG during symbiosis.

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Overexpression of class 1 plant hemoglobin genes enhances symbiotic nitrogen fixation activity betweenMesorhizobium lotiandLotus japonicus

The Plant Journal ◽

10.1111/j.1365-313x.2008.03689.x ◽

2009 ◽

Vol 57 (2) ◽

pp. 254-263 ◽

Cited By ~ 70

Author(s):

Yoshikazu Shimoda ◽

Fuyuko Shimoda-Sasakura ◽

Ken-ichi Kucho ◽

Norihito Kanamori ◽

Maki Nagata ◽

...

Keyword(s):

Nitrogen Fixation ◽

Symbiotic Nitrogen Fixation ◽

Nitrogen Fixation Activity ◽

Fixation Activity ◽

Class 1

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Effect of abscisic acid on symbiotic nitrogen fixation activity in the root nodules ofLotus japonicus

Plant Signaling & Behavior ◽

10.4161/psb.5.4.10849 ◽

2010 ◽

Vol 5 (4) ◽

pp. 440-443 ◽

Cited By ~ 16

Author(s):

Akiyoshi Tominaga ◽

Maki Nagata ◽

Koichi Futsuki ◽

Hidetoshi Abe ◽

Toshiki Uchiumi ◽

...

Keyword(s):

Nitrogen Fixation ◽

Abscisic Acid ◽

Symbiotic Nitrogen Fixation ◽

Root Nodules ◽

Nitrogen Fixation Activity ◽

Fixation Activity

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Quantitative trait locus analysis of symbiotic nitrogen fixation activity in the model legume Lotus japonicus

Journal of Plant Research ◽

10.1007/s10265-011-0459-1 ◽

2011 ◽

Vol 125 (3) ◽

pp. 395-406 ◽

Cited By ~ 6

Author(s):

Akiyoshi Tominaga ◽

Takahiro Gondo ◽

Ryo Akashi ◽

Shao-hui Zheng ◽

Susumu Arima ◽

...

Keyword(s):

Quantitative Trait Locus ◽

Nitrogen Fixation ◽

Quantitative Trait Locus Analysis ◽

Quantitative Trait ◽

Symbiotic Nitrogen Fixation ◽

Lotus Japonicus ◽

Model Legume ◽

Nitrogen Fixation Activity ◽

Fixation Activity ◽

Trait Locus

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Root Distribution and Nitrogen Fixation Activity of Tropical Forage Legume American Jointvetch (Aeschynomene americanaL.) cv. Glenn under Waterlogging Conditions

International Journal of Agronomy ◽

10.1155/2014/507405 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Manabu Tobisa ◽

Masataka Shimojo ◽

Yasuhisa Masuda

Keyword(s):

Nitrogen Fixation ◽

Root Distribution ◽

Water Surface ◽

Nitrogenase Activity ◽

Soil Surface ◽

Nodule Formation ◽

Nitrogen Fixation Activity ◽

Fixation Activity ◽

Net Assimilation ◽

Waterlogging Treatment

We investigated the root distribution and nitrogen fixation activity of American jointvetch (Aeschynomene americanaL.) cv. Glenn, under waterlogging treatment. The plants were grown in pots under three different treatments: no waterlogging (control), 30 days of waterlogging (experiment 1), and 40 days of waterlogging (experiment 2). The plants were subjected to the treatments on day 14 after germination. Root dry matter (DM) weight distribution of waterlogged plants was shallower than controls after day 20 of waterlogging. Throughout the study period, the total root DM weight in waterlogged plants was similar to that in the controls. Enhanced rooting (adventitious roots) and nodule formation at the stem base were observed in waterlogged plants after day 20 of waterlogging. The average DM weight of individual nodules on the region of the stem between the soil surface and water surface of waterlogged plants was similar to that of individual taproot nodules in the controls. Waterlogged plants had slightly greater plant DM weight than the controls after 40 days of treatment. The total nitrogenase activity (TNA) of nodules and nodule DM weight were higher in waterlogged plants than in the controls. Waterlogged American jointvetch had roots with nodules both around the soil surface and in the area between the soil surface and water surface after 20 days of waterlogging, and they maintained high nitrogenase activity and net assimilation rate that resulted in an increased growth rate.

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The effect of inoculation of pea plants with mycorrhizal fungi and Rhizobium on nitrogen and phosphorus assimilation

Plant Soil and Environment ◽

10.17221/3463-pse ◽

2011 ◽

Vol 52 (No. 10) ◽

pp. 435-440 ◽

Cited By ~ 27

Author(s):

M. Geneva ◽

G. Zehirov ◽

E. Djonova ◽

N. Kaloyanova ◽

G. Georgiev ◽

...

Keyword(s):

Nitrogen Fixation ◽

Mycorrhizal Fungi ◽

Glomus Mosseae ◽

Rhizobium Leguminosarum ◽

Glomus Intraradices ◽

Plant Biomass ◽

Phosphorus Level ◽

Nitrogen And Phosphorus ◽

Nitrogen Fixation Activity ◽

Fixation Activity

The study evaluated the response of pea (Pisum sativum cv. Avola) to arbuscular mycorrhizal fungi (AM) species Glomus mosseae and Glomus intraradices and Rhizobium leguminosarum bv. viceae, strain D 293, regarding the growth, photosynthesis, nodulation and nitrogen fixation activity. Pea plants were grown in a glasshouse until the flowering stage (35 days), in 4 kg plastic pots using leached cinnamonic forest soil (Chromic Luvisols – FAO) at P levels 13.2 (P1) and 39.8 (P2) mg P/kg soil. The obtained results demonstrated that the dual inoculation of pea plants significantly increased the plant biomass, photosynthetic rate, nodulation, and nitrogen fixation activity in comparison with single inoculation with Rhizobium leguminosarum bv. viceae strain D 293. On the other hand, coinoculation significantly increased the total phosphorus content in plant tissue, acid phosphatase activity and percentage of root colonization. The effectiveness of coinoculation with Rhizobium leguminosarum and Glomus mosseae was higher at the low phosphorus level while the coinoculation with Glomus intraradices appeared to be the most effective at higher phosphorus level.

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