Spectrophotometric Determination of Protein by the Stopped-Flow Time Difference Analysis Technique to Enhance the Sensitivity of the Coomassie Brilliant Blue-Binding Method

1988 ◽  
Vol 52 (10) ◽  
pp. 2615-2616
Author(s):  
Ken-ichiro Kanaya ◽  
Keitaro Hiromi
Keyword(s):  
Spectrophotometric Determination ◽  
Flow Time ◽  
Time Difference ◽  
Brilliant Blue ◽  
Stopped Flow ◽  
Difference Analysis ◽  
Coomassie Brilliant Blue ◽  
Analysis Technique ◽  
Coomassie Brilliant

Total protein determined in human breast milk by use of coomassie brilliant blue and centrifugal analysis.

1989 ◽  
Vol 35 (10) ◽  
pp. 2127-2129 ◽  
Author(s):  
Y Bergqvist ◽  
L Karlsson ◽  
L Fohlin
Keyword(s):  
Breast Milk ◽  
Total Protein ◽  
Brilliant Blue ◽  
Human Breast Milk ◽  
Human Breast ◽  
Simple Method ◽  
Coomassie Brilliant Blue ◽  
Kjeldahl Method ◽  
Coomassie Brilliant

Abstract This simple method of centrifugal analysis for total protein in human breast milk is based on the change in the wavelength of the absorbance maximum of Coomassie Brilliant Blue G-250 when the dye is bound to protein. Within-run and between-day CVs were 3.8% and 4.8%, respectively. Compared with a micro-Kjeldahl method for determination of total nitrogen, the coefficient of correlation was 0.99.

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