scholarly journals NifA Protein SynthesizedIn VitroBinds to the Upstream Activator Sequence in the Promoter of theKlebsiella oxytoca nifB Gene

1991 ◽  
Vol 55 (12) ◽  
pp. 3121-3123
Author(s):  
Young-Mi Kim ◽  
Makoto Hidaka ◽  
Haruhiko Masaki ◽  
Teruhiko Beppu ◽  
Takeshi Uozumi
Keyword(s):  
Nifa Protein

Use of lactose to induce expression of soluble NifA protein domains of Herbaspirillum seropedicae in Escherichia coli

2000 ◽  
Vol 46 (11) ◽  
pp. 1087-1090 ◽  
Author(s):  
Rose A. Monteiro ◽  
Emanuel M. Souza ◽  
M. Geoffrey Yates ◽  
Fábio O. Pedrosa ◽  
Leda S. Chubatsu
Keyword(s):  
Escherichia Coli ◽  
Protein Domains ◽  
Herbaspirillum Seropedicae ◽  
Nifa Protein

scholarly journals Inter-domain cross-talk controls the NifA protein activity ofHerbaspirillum seropedicae

FEBS Letters ◽  
2001 ◽  
Vol 508 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Rose A. Monteiro ◽  
Emanuel M. de Souza ◽  
Roseli Wassem ◽  
M.Geoffrey Yates ◽  
Fabio O. Pedrosa ◽  
...  
Keyword(s):  
Cross Talk ◽  
Protein Activity ◽  
Nifa Protein

scholarly journals Expression and functional analysis of an N-truncated NifA protein ofHerbaspirillum seropedicae

FEBS Letters ◽  
1999 ◽  
Vol 447 (2-3) ◽  
pp. 283-286 ◽  
Author(s):  
R.A Monteiro ◽  
E.M Souza ◽  
S Funayama ◽  
M.G Yates ◽  
F.O Pedrosa ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Nifa Protein

scholarly journals In-transregulation of the N-truncated-NIFA protein ofHerbaspirillum seropedicaeby the N-terminal domain

1999 ◽  
Vol 180 (2) ◽  
pp. 157-161 ◽  
Author(s):  
Rose Adele Monteiro ◽  
Emanuel M Souza ◽  
M.Geoffrey Yates ◽  
Fábio O Pedrosa ◽  
Leda S Chubatsu
Keyword(s):  
Terminal Domain ◽  
Nifa Protein

Critical spacing between two essential cysteine residues in the interdomain linker of the Bradyrhizobium japonicum NifA protein

FEBS Letters ◽  
1989 ◽  
Vol 255 (1) ◽  
pp. 167-171 ◽  
Author(s):  
Hans-Martin Fischer ◽  
Stefan Fritsche ◽  
Brigitte Herzog ◽  
Hauke Hennecke
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Cysteine Residues ◽  
Critical Spacing ◽  
Nifa Protein ◽  
Interdomain Linker

scholarly journals NifA Protein Synthesized In Vitro Binds to the Upstream Activator Sequence in the Promoter of the Klebsiella oxytoca nifB Gene.

1991 ◽  
Vol 55 (12) ◽  
pp. 3121-3123 ◽  
Author(s):  
Young-Mi KIM ◽  
Makoto HIDAKA ◽  
Haruhiko MASAKI ◽  
Teruhiko BEPPU ◽  
Takeshi UOZUMI
Keyword(s):  
Klebsiella Oxytoca ◽  
Nifa Protein

scholarly journals Fnr Is Involved in Oxygen Control of Herbaspirillum seropedicae N-Truncated NifA Protein Activity in Escherichia coli

2003 ◽  
Vol 69 (3) ◽  
pp. 1527-1531 ◽  
Author(s):  
Rose A. Monteiro ◽  
Emanuel M. de Souza ◽  
M. Geoffrey Yates ◽  
Fabio O. Pedrosa ◽  
Leda S. Chubatsu
Keyword(s):  
Escherichia Coli ◽  
Protein A ◽  
Ammonium Ions ◽  
Oxygen Control ◽  
Herbaspirillum Seropedicae ◽  
Protein Activity ◽  
Iron Deprivation ◽  
Iron Requirement ◽  
Fnr Protein ◽  
Nifa Protein

ABSTRACT Herbaspirillum seropedicae is an endophytic diazotroph belonging to the β-subclass of the class Proteobacteria, which colonizes many members of the Gramineae. The activity of the NifA protein, a transcriptional activator of nif genes in H. seropedicae, is controlled by ammonium ions through its N-terminal domain and by oxygen through mechanisms that are not well understood. Here we report that the NifA protein of H. seropedicae is inactive and more susceptible to degradation in an fnr Escherichia coli background. Both effects correlate with oxygen exposure and iron deprivation. Our results suggest that the oxygen sensitivity and iron requirement for H. seropedicae NifA activity involve the Fnr protein.

Expression, purification, and functional analysis of the C-terminal domain of Herbaspirillum seropedicae NifA protein

2003 ◽  
Vol 27 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Rose A Monteiro ◽  
Emanuel M Souza ◽  
M Geoffrey Yates ◽  
M.Berenice R Steffens ◽  
Fábio O Pedrosa ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Herbaspirillum Seropedicae ◽  
Terminal Domain ◽  
Nifa Protein
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