Continuous Lactic Acid Production from Raw Starch in a Fermentation System Using a Reversibly Soluble-autoprecipitating Amylase and Immobilized Cells ofLactobacillus casei

1991 ◽  
Vol 55 (2) ◽  
pp. 479-485 ◽  
Author(s):  
Kazuhiro Hoshino ◽  
Masayuki Taniguchi ◽  
Hideji Marumoto ◽  
Kazuyuki Shimizu ◽  
Michihiro Fujii
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Immobilized Cells ◽  
Lactic Acid Production ◽  
Raw Starch ◽  
Fermentation System

Food Additive Lactic Acid Production by Immobilized Cells ofLactobacillus brevison Delignified Cellulosic Material

2003 ◽  
Vol 51 (18) ◽  
pp. 5285-5289 ◽  
Author(s):  
Ornel Elezi ◽  
Yiannis Kourkoutas ◽  
Athanasios A. Koutinas ◽  
Maria Kanellaki ◽  
Eugenia Bezirtzoglou ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Immobilized Cells ◽  
Lactic Acid Production ◽  
Food Additive ◽  
Cellulosic Material

Efficient d-lactic acid production from raw starch

2009 ◽  
Vol 108 ◽  
pp. S47-S48 ◽  
Author(s):  
Satoru Shinkawa ◽  
Kenji Okano ◽  
Tsutomu Tanaka ◽  
Chiaki Ogino ◽  
Akihiko Kondo
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Raw Starch

scholarly journals Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

2020 ◽  
Vol 8 (5) ◽  
pp. 717
Author(s):  
Aleksandra Gęsicka ◽  
Monika Borkowska ◽  
Wojciech Białas ◽  
Paulina Kaczmarek ◽  
Ewelina Celińska
Keyword(s):  
Lactic Acid ◽  
Ethanol Production ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Thermal Conditions ◽  
Cell Factory ◽  
The Novel ◽  
Microbial Cell Factory ◽  
Raw Starch

Sustainable economy drives increasing demand for raw biomass-decomposing enzymes. Microbial expression platforms exploited as cellular factories of such biocatalysts meet requirements of large-volume production. Previously, we developed Yarrowia lipolytica recombinant strains able to grow on raw starch of different plant origin. In the present study, we used the most efficient amylolytic strain as a microbial cell factory of raw-starch-digesting (RSD) amylolytic preparation composed of two enzymes. The RSD-preparation was produced in fed-batch bioreactor cultures. Concentrated and partly purified preparation was then tested in simultaneous saccharification and fermentation (SSF) processes with thermotolerant Kluyveromyces marxianus for ethanol production and Lactobacillus plantarum for production of lactic acid. These processes were conducted as a proof-of-concept that application of the novel RSD-preparation supports sufficient starch hydrolysis enabling microbial growth and production of targeted molecules, as the selected strains were confirmed to lack amylolytic activity. Doses of the preparation and thermal conditions were individually adjusted for the two processes. Additionally, ethanol production was tested under different aeration strategies; and lactic acid production process was tested in thermally pre-treated substrate, as well. Conducted studies demonstrated that the novel RSD-preparation provides satisfactory starch hydrolyzing activity for ethanol and lactic acid production from starch by non-amylolytic microorganisms.

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