scholarly journals Production of Raw Starch-Digesting Amylolytic Preparation in Yarrowia lipolytica and Its Application in Biotechnological Synthesis of Lactic Acid and Ethanol

2020 ◽  
Vol 8 (5) ◽  
pp. 717
Author(s):  
Aleksandra Gęsicka ◽  
Monika Borkowska ◽  
Wojciech Białas ◽  
Paulina Kaczmarek ◽  
Ewelina Celińska
Keyword(s):  
Lactic Acid ◽  
Ethanol Production ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Thermal Conditions ◽  
Cell Factory ◽  
The Novel ◽  
Microbial Cell Factory ◽  
Raw Starch

Sustainable economy drives increasing demand for raw biomass-decomposing enzymes. Microbial expression platforms exploited as cellular factories of such biocatalysts meet requirements of large-volume production. Previously, we developed Yarrowia lipolytica recombinant strains able to grow on raw starch of different plant origin. In the present study, we used the most efficient amylolytic strain as a microbial cell factory of raw-starch-digesting (RSD) amylolytic preparation composed of two enzymes. The RSD-preparation was produced in fed-batch bioreactor cultures. Concentrated and partly purified preparation was then tested in simultaneous saccharification and fermentation (SSF) processes with thermotolerant Kluyveromyces marxianus for ethanol production and Lactobacillus plantarum for production of lactic acid. These processes were conducted as a proof-of-concept that application of the novel RSD-preparation supports sufficient starch hydrolysis enabling microbial growth and production of targeted molecules, as the selected strains were confirmed to lack amylolytic activity. Doses of the preparation and thermal conditions were individually adjusted for the two processes. Additionally, ethanol production was tested under different aeration strategies; and lactic acid production process was tested in thermally pre-treated substrate, as well. Conducted studies demonstrated that the novel RSD-preparation provides satisfactory starch hydrolyzing activity for ethanol and lactic acid production from starch by non-amylolytic microorganisms.

scholarly journals Chirality Matters: Synthesis and Consumption of the d-Enantiomer of Lactic Acid by Synechocystis sp. Strain PCC6803

2015 ◽  
Vol 82 (4) ◽  
pp. 1295-1304 ◽  
Author(s):  
S. Andreas Angermayr ◽  
Aniek D. van der Woude ◽  
Danilo Correddu ◽  
Ramona Kern ◽  
Martin Hagemann ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactate Dehydrogenase ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Cell Factory ◽  
Growth Stages ◽  
Microbial Cell Factory ◽  
Content Type ◽  
Acid Consumption ◽  
Glycolate Dehydrogenase

ABSTRACTBoth enantiomers of lactic acid,l-lactic acid andd-lactic acid, can be produced in a sustainable way by a photosynthetic microbial cell factory and thus from CO2, sunlight, and water. Several properties of polylactic acid (a polyester of polymerized lactic acid) depend on the controlled blend of these two enantiomers. Recently, cyanobacteriumSynechocystissp. strain PCC6803 was genetically modified to allow formation of either of these two enantiomers. This report elaborates on thed-lactic acid production achieved by the introduction of ad-specific lactate dehydrogenase from the lactic acid bacteriumLeuconostoc mesenteroidesintoSynechocystis. A typical batch culture of this recombinant strain initially shows lactic acid production, followed by a phase of lactic acid consumption, until production “outcompetes” consumption at later growth stages. We show thatSynechocystisis able to used-lactic acid, but notl-lactic acid, as a carbon source for growth. Deletion of the organism's putatived-lactate dehydrogenase (encoded byslr1556), however, does not eliminate this ability with respect tod-lactic acid consumption. In contrast,d-lactic acid consumption does depend on the presence of glycolate dehydrogenase GlcD1 (encoded bysll0404). Accordingly, this report highlights the need to match a product of interest of a cyanobacterial cell factory with the metabolic network present in the host used for its synthesis and emphasizes the need to understand the physiology of the production host in detail.

Efficient d-lactic acid production from raw starch

2009 ◽  
Vol 108 ◽  
pp. S47-S48 ◽  
Author(s):  
Satoru Shinkawa ◽  
Kenji Okano ◽  
Tsutomu Tanaka ◽  
Chiaki Ogino ◽  
Akihiko Kondo
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Raw Starch

Monascus ruber as cell factory for lactic acid production at low pH

2017 ◽  
Vol 42 ◽  
pp. 66-73 ◽  
Author(s):  
Ruud A. Weusthuis ◽  
Astrid E. Mars ◽  
Jan Springer ◽  
Emil JH Wolbert ◽  
Hetty van der Wal ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Low Ph ◽  
Cell Factory ◽  
Monascus Ruber

scholarly journals Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mayara Souza Silva ◽  
Aline Silva Braga ◽  
Patrícia Sanches Kerges Bueno ◽  
Paulo Sergio da Silva Santos ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Protective Effect ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Negative Control ◽  
Titanium Tetrafluoride ◽  
Silver Diamine Fluoride ◽  
Dentin Caries ◽  
Radiation Induced

AbstractThis in vitro study evaluated the protective effect of titanium tetrafluoride (TiF4) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF4 varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 °C, 5% CO2) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p < 0.05). TiF4 and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF4 and SDF significantly reduced the biofilm viability compared to negative control. TiF4 significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF4 and SDF may be good alternatives to control the development of radiation-induced dentin caries.

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