scholarly journals 3D analysis of the myenteric plexus of the human bowel by X-ray phase-contrast tomography – a future method?

2020 ◽  
Vol 55 (10) ◽  
pp. 1261-1267
Author(s):  
Niccolò Peruzzi ◽  
Béla Veress ◽  
Lars B. Dahlin ◽  
Tim Salditt ◽  
Mariam Andersson ◽  
...  
2021 ◽  
Author(s):  
Matthieu Chourrout ◽  
Margaux Roux ◽  
Carlie Boisvert ◽  
Coralie Gislard ◽  
David Legland ◽  
...  

While numerous transgenic mouse strains have been produced to model the formation of amyloid-β (Aβ) plaques in the brain, efficient methods for whole-brain 3D analysis of Aβ deposits are lacking. Moreover, standard immunohistochemistry performed on brain slices precludes any shape analysis of Aβ plaques. The present study shows how in-line (propagation-based) X-ray phase-contrast tomography (XPCT) combined with ethanol-induced brain sample dehydration enables hippocampus-wide detection and morphometric analysis of Aβ plaques. Performed in three distinct Alzheimer mouse strains, the proposed workflow identified differences in signal intensity and 3D shape parameters: 3xTg displayed a different type of Aβ plaques, with a larger volume and area, greater elongation, flatness and mean breadth, and more intense average signal than J20 and APP/PS1. As a label-free non-destructive technique, XPCT can be combined with standard immunohistochemistry. XPCT virtual histology could thus become instrumental in quantifying the 3D spreading and the morphological impact of seeding when studying prion-like properties of Aβ aggregates in animal models of Alzheimer's disease. This is Part II of a series of two articles reporting the value of in-line XPCT for virtual histology of the brain; Part I shows how in-line XPCT enables 3D myelin mapping in the whole rodent brain and in human autopsy brain tissue.


Author(s):  
Inna Bukreeva ◽  
Graziano Ranocchia ◽  
Vincenzo Formoso ◽  
Michele Alessandrelli ◽  
Michela Fratini ◽  
...  

ACS Nano ◽  
2016 ◽  
Vol 10 (8) ◽  
pp. 7990-7997 ◽  
Author(s):  
Fu Sun ◽  
Lukas Zielke ◽  
Henning Markötter ◽  
André Hilger ◽  
Dong Zhou ◽  
...  

2012 ◽  
Vol 18 (S2) ◽  
pp. 516-517
Author(s):  
J. Gelb ◽  
B. Hornberger ◽  
C. Holzner ◽  
A. Merkle ◽  
M. Feser ◽  
...  

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.


2018 ◽  
Vol 115 (27) ◽  
pp. 6940-6945 ◽  
Author(s):  
Mareike Töpperwien ◽  
Franziska van der Meer ◽  
Christine Stadelmann ◽  
Tim Salditt

To quantitatively evaluate brain tissue and its corresponding function, knowledge of the 3D cellular distribution is essential. The gold standard to obtain this information is histology, a destructive and labor-intensive technique where the specimen is sliced and examined under a light microscope, providing 3D information at nonisotropic resolution. To overcome the limitations of conventional histology, we use phase-contrast X-ray tomography with optimized optics, reconstruction, and image analysis, both at a dedicated synchrotron radiation endstation, which we have equipped with X-ray waveguide optics for coherence and wavefront filtering, and at a compact laboratory source. As a proof-of-concept demonstration we probe the 3D cytoarchitecture in millimeter-sized punches of unstained human cerebellum embedded in paraffin and show that isotropic subcellular resolution can be reached at both setups throughout the specimen. To enable a quantitative analysis of the reconstructed data, we demonstrate automatic cell segmentation and localization of over 1 million neurons within the cerebellar cortex. This allows for the analysis of the spatial organization and correlation of cells in all dimensions by borrowing concepts from condensed-matter physics, indicating a strong short-range order and local clustering of the cells in the granular layer. By quantification of 3D neuronal “packing,” we can hence shed light on how the human cerebellum accommodates 80% of the total neurons in the brain in only 10% of its volume. In addition, we show that the distribution of neighboring neurons in the granular layer is anisotropic with respect to the Purkinje cell dendrites.


2019 ◽  
Vol 82 (7) ◽  
pp. 953-960
Author(s):  
Hong‐Xia Yin ◽  
Peng Zhang ◽  
Zheng Wang ◽  
Yun‐Fu Liu ◽  
Ying Liu ◽  
...  

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