Preparative Separation and Purification of Three Flavonoids from the Anti-Inflammatory Effective Fraction ofSmilax chinaL. by High-Speed Counter-Current Chromatography

2014 ◽  
Vol 49 (13) ◽  
pp. 2090-2097 ◽  
Author(s):  
Yinzhou Fan ◽  
Haiyan Xiang ◽  
Yanqin Luo ◽  
Luyao Song ◽  
Yao Liu ◽  
...  
Keyword(s):  
High Speed ◽  
Preparative Separation ◽  
Separation And Purification ◽  
Effective Fraction ◽  
Anti Inflammatory ◽  
Counter Current

Preparative Separation and Purification of Lancifodilactone C from Schisandra Chinensis (Turcz.) Baill by High-Speed Counter-Current Chromatography

2013 ◽  
Vol 634-638 ◽  
pp. 1502-1505
Author(s):  
Bin Li ◽  
Xian Jun Meng ◽  
Li Jie Zhu ◽  
Xin Yao Jiao
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Butyl Alcohol ◽  
Structure Identification ◽  
Schisandra Chinensis ◽  
Preparative Separation ◽  
Separation And Purification ◽  
Two Phase ◽  
H Nmr ◽  
Counter Current

High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of lancifodilactone C from the crude extracts of Schisandra chinensis (Turcz.) Baill. Following an initial cleaning-up step on the AB-8 macroporous resin, a preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of Chloroform- n-Butyl alcohol- methanol -water(10:0.5:8:4，v/v) was used to isolate and separate lancifodilactone C from Schisandra Chinensis(Turcz.) Baill. A total of 101 mg lancifodilactone C with purities of 98.2% were obtained from 1000 mg crude extract in one-step elution and less than 1 h, and the structure identification was performed by UV, IR, MS,1H NMR and13C NMR.

Sign in / Sign up

Export Citation Format

Share Document