The copper ore, which has fine-grained nature and differences in the degree of mineral dissemination, is a kind of low grade sulfide minerals. Tests indicate that not only the grinding fineness but also the combination mode of depressants is one of the most important factors to improve the concentrate grade index. Additionally, according to tests conducted with dosage of lime, the rougher flotation should be operated at a pH of 11. Furthermore, all the depressants used were effective to increase the concentrate grade. By the closed-circuit micro-flotation experiment, satisfied grade index (18.7%Cu with 81% recovery) of the final concentrate was achieved, which could provide reference in industrial applications.