Conservation strategies for threatened medicinal plant –Barleria prionitisL. – using in vitro and ex vitro propagation techniques

2012 ◽  
Vol 45 (11) ◽  
pp. 1327-1340 ◽  
Author(s):  
Shabir A. Lone ◽  
A.S. Yadav ◽  
A. Bajaj ◽  
Ajit K. Sharma ◽  
Yogesh Badkhane ◽  
...  
2018 ◽  
Vol 77 (1) ◽  
pp. 80-87 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari

AbstractHybanthus enneaspermusis a rare medicinal plant. We defined a protocol for micropropagation,ex vitrorooting of cloned shoots and their acclimatization. Surface-sterilized nodal segments were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) and kinetin (Kin). Medium supplemented with 1.5 mg L−1BAP was found optimum for shoot induction from the explants and 6.4±0.69 shoots were regenerated from each node with 97% response. Shoots were further proliferated maximally (228±10.3 shoots per culture bottle with 7.5±0.43 cm length) on MS medium augmented with 1.0 mg L−1each of BAP and Kin within 4–5 weeks. The shoots were rootedin vitroon half strength MS medium containing 2.0 mg L−1indole-3 butyric acid (IBA). The cloned shoots were pulse-treated with 300 mg L–1 of IBA and cultured on soilrite® in a greenhouse. About 96% of the IBA-pulsed shoots rootedex vitroin soilrite®, each shoot producing 12.5±0.54 roots with 5.1±0.62 cm length. Theex vitrorooted plantlets showed a better rate of survival (92%) in a field study thanin vitrorooted plantlets (86%). A comparative foliar micromorphological study ofH. enneaspermuswas conducted to understand the micromorphological changes during plant developmental processes fromin vitrotoin vivoconditions in terms of variations in stomata, vein structures and spacing, and trichomes. This is the first report onex vitrorooting inH. enneaspermusand the protocol can be exploited for conservation and large-scale propagation of this rare and medicinally important plant.


2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Manisha Mathela ◽  
Amit Kumar ◽  
Monika Sharma ◽  
Gurinderjit Singh Goraya

AbstractThe unique Himalayan ecosystems are repositories to the wild populations of diverse flora and fauna. The high value medicinal and aromatic plant species (MAPs) are an example of the same. Since time immemorial, these MAPs have been traditionally used by the local inhabitants and have eventually developed a high market value all over the world. Increasing market demand engenders over-extraction of species, unsustainable collection further catalyses decline in wild populations. The current communication raises high conservation concern on the rapid population decline of Fritillaria cirrhosa D.Don in the Western Himalaya. Harvested and traded with a new trade name i.e., ‘Jangli lehsun’ probably to disguise common Allium species, the species is facing tremendous decline in wild populations due to its illegal harvesting and trade in Himachal Pradesh. Further, F. cirrhosa faces threat due to unorganized, over-extraction, unsustainable and premature harvesting of the bulbs, coupled with illegal hidden markets functioning parallelly. Considering that this valuable species is under multiple threats being a medicinally important plant, priority should be given for its conservation through in-situ such as identification of medicinal plant conservation areas and ex-situ methods for its propagation and multiplication. Further, to ensure the long-term conservation of Fritillaria cirrhosa, prioritized conservation strategies such as strengthening of the Biodiversity Management Committees, capacity building through awareness programs for the key stakeholders and sustainable harvesting would be the practical solution.


2015 ◽  
pp. 99-112
Author(s):  
Marija Markovic ◽  
Dragana Skocajic ◽  
Mihailo Grbic ◽  
Matilda Djukic ◽  
Dragica Obratov-Petkovic ◽  
...  

The aim of this study was to determine the possibility of micropropagation of the medicinal plant A. millefolium on half-strength MS medium and ex vitro rooting and acclimatization of the obtained microshoots in hydroculture in order to establish an efficient production method. Two explant types were used: basal and terminal cuttings, and better results were achieved when terminal cuttings were used. The development of shoots in the multiplication phase was successful with a regeneration percentage of 100%. Ex vitro rooting in a modified Hoagland nutrient solution was successful (83%), but the percentage of in vitro rooting on half-strength MS medium without hormones was higher (95%). However, bearing in mind that mass production of A. millefolium is more efficient when the phase of in vitro rooting is excluded, this method could be recommended for commercial propagation of this medicinal plant. It is necessary to conduct additional research in order to optimize the composition, EC and pH value of the hydroponic nutrient solution.


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