A new addition to the alien flora of Tunisia, Amaranthus spinosus L. (Amaranthaceae s.l.), with notes on A. diacanthus Raf.

Amaranthus spinosus L. (Amaranthaceae s.l.), a species native to the Neotropics, has been found in four localities (Bizerta, Bir Bouregba, Hammamet, and Nabeul) of N. Tunisia. Our discovery represents the first record at national level, and the second one for N. Africa. Morphological characters and ecological data are given. Nomenclatural notes are provided for the name A. diacanthus, which was regarded by some authors as heterotypic synonym of A. spinosus. A neotype is designated in the present paper based on a specimen preserved at LSU.

Expression of small GTPases in the roots and nodules of Medicago truncatula cv. Jemalong

In this study we used Medicago truncatula, to identify and analyze the expression of small GTP-binding proteins (Arf1, Arl1, Sar1, Rabs, Rop/Rac) and their interacting partners in the infection process in the roots and nodules. A real-time polymerase chain reaction analysis was carried out and our results showed that Arf1 (AtArfB1c-like), MtSar1, AtRabA1e-like, AtRabC1-like, MsRab11-like and AtRop7-like genes were highly expressed in the nodules of rhizobium inoculated plants compared to the non-inoculated ones. On the contrary, AtRabA3 like, AtRab5c and MsRac1-like genes were highly expressed in non-infected nitrogen supplied roots of M. truncatula. Other Rab genes (AtRabA4a, AtRabA4c and AtRabG3a-like genes) were nearly equally expressed in both treatments. Interestingly, RbohB (a respiratory burst NADPH oxidase homologue) was more highly expressed in rhizobium infected than in non-infected roots and nodules. Our data show a differential expression pattern of small GTP-binding proteins in roots and nodules of the plants. This study demonstrates an important role of small GTP-binding proteins in symbiosome biogenesis and root nodule development in legumes.

Distribution of Taraxacum microspecies along soil property gradients in salt and brackish meadows on the Polish Baltic coast

The vegetation of protected salt meadows along the Baltic coast is fairly well known; however, dandelions have been so far treated as a collective species. The aim of our study was to examine the microspecies diversity of the genus Taraxacum in Polish salt and brackish coastal meadows and to analyse soil property preferences of the dandelion microspecies identified. In addition, we analysed the relations between soil properties and vegetation patterns in dandelion-supporting coastal meadows (by canonical correspondence analysis). The salt and brackish meadows along the Polish Baltic coast we visited were found to support a total of 27 dandelion microspecies representing 5 sections. Analysis of vegetation patterns showed all the soil parameters (C:N ratio, organic matter content, pH, concentration of Mg, P, K, electrolytic conductivity of the saturated soil extract ECe) to explain 32.07% of the total variance in the species data. The maximum abundance of most dandelion microspecies was associated with the highest soil fertility, moderate pH values and organic matter content, and with the lowest magnesium content and soil salinity. The exceptions were T. latissimum, T. stenoglossum, T. pulchrifolium and T. lucidum the occur-rence of which was related to the lowest soil fertility and the highest salinity. In addition, several microspecies (T. leptodon, T. gentile, T. haematicum, T. fusciflorum and T. balticum) were observed at moderate C:N ratios and ECe. Four other microspecies (T. infestum, T. cordatum, T. hamatum, T. sertatum) occurred at the lowest pH and organic matter content. The information obtained increases the still insufficient body of knowledge on ecological spectra of individual dandelion microspecies, hence their potential indicator properties.

A cytogenetic and pollen study of annual Medicago species from Soummam Valley (Northeastern of Algeria)

This paper reports a cytogenetic study of eight Medicago L. species sampled from the Soummam Valley (northeastern Algeria). Chromosome numbers and meiosis irregularities during microsporogenesis were explored. Pollen viability rate and pollen size were also examined. The studied taxa are diploid and display biva-lent pairing and regular chromosome segregation during meiosis. Although meiosis appears regular, some anomalies were detected in relatively high cumulated rates (14.66%–26.14%). The most common meiotic abnormality examined here is related to cytomixis (from 14.66% in M. littoralis to 25.83% in M. laciniata). Other anomalies were also detected, including chromatic bridges, asynchronous divisions, micronuclei and multipolar cells. Consequently, the species exhibited varying percentages of pollen viability (from 70.11% in M. laciniata to 99.14% in M. littoralis). Pollen viability was negatively correlated with meiotic abnormalities (Pearson correlation coefficient R = −0.72, p = 0.043). The pollen grains were also heterogeneous in size. Medicago truncatula Gaertn. and M. laciniata (L.) Miller presented the most variable pollen size (relative standard deviation exceeding 19%). Medicago littoralis is distinguished from other species by possessing homogeneous and large sized pollen (relative standard deviation RSD = 6.73 %). The cytogenetic and pollen data provided by this study are discussed in the context of species systematics and in the perspective of genetic improvement.

Setaria adhaerens (Forssk.) Chiov. (Poaceae), a new alien species in the Croatian flora

Setaria adhaerens (Forssk.) Chiov. a pantropical plant species present in some parts of the European continent has been recorded for the first time in Croatia in two anthropogenic habitats in Dalmatia and one in Kvarner bay. The paper presents a short morphological description and photographs of the new alien species, as well as its distribution. A determination key is given for the Setaria taxa most frequently found in Europe.

Morpho-chemical divergence and fatty acid profile of shea tree seeds (Vitellaria paradoxa) collected from different locations in Kwara State, Nigeria

The present study characterizes seed-related traits, phytochemical, physiochemical parameters and fatty acid profile of shea (Vitellaria paradoxa) seeds collected from the Kosubosu, Fufu and Sare areas of Kwara State, Nigeria to determine the effects of microclimate on seed morphology, biochemical and oil constituents. Seed morphological data were analyzed for variability. Seed oil was extracted for phytochemical constituents, physicochemical properties, and fatty acid profiling by gas chromatography equipped with mass spectrometry (GC/MS). Results showed intra and inter-locational variations in seed characters. Most fruits had 1–2 seeds. Seeds were predominantly brown and very few were dark brown. Phytochemicals and physicochemical parameters of the seed oil varied with place of collection. Alkaloid, saponin, tannin and phytate contents ranged between 0.79–0.84, 1.20–1.26, 1.48–1.56 and 0.15–0.18 mg g−1 respectively. The density of the oil was less than that of water, acid value ranged from 10.58–13.56 mg KOH g−1 and iodine values were between 36.63 to 40.32 g I2 (100 g)–1. Saponification values lie between 160.39 and 184.14 mg KOH g−1; and free fatty acid was within 5.32–6.81 %. Peroxide, ɑ-tocopherol, total phenol and oxalate values as well as viscosity of the oil also varied; however, refractive index was similar. Ethyl oleate and octadecanoic acids were present and most abundance in all the locations, while glycidol stearate was only found in Fufu samples with three other fatty acids. Five fatty acids were present in Kosubosu, while Sare had only two. The results obtained in the present study indicate that shea oil could be used for medicinal, nutritional and industrial purposes. Since seed characters, phytochemical, physicochemical and fatty acid compositions varied with the microclimate, environmental and micro-ecological conditions should be considered when collecting seeds for oil utilization.

Morphology and seed protein profile for a new species of the genus Cleome L. (Cleomaceae) from Pakistan

A new species of the genus Cleome L. from Pakistan is described and illustrated. The new species is described under the name C. karachiensis sp. nov. and compared with two closely related species i.e., Cleome brachycarpa and C. viscosa in terms of morphology, palynology, seed morphology and seed protein profile. A key to the species of genus Cleome L. from Pakistan is also provided.

Rocky coastal vegetation of the class Crithmo-Staticetea in the south-east of Italy

In the south-east of Italy, rocky coasts are almost entirely concentrated in the Apulia region. Several phytosociological papers have already dealt with the rocky coastal vegetation of the class Crithmo-Staticetea in some parts of the Apulian coast. However, there is still no overall revision carried out by using modern statistical treatments of phytosociological data. This paper aims to revise the syntaxonomy and nomenclature of the class Crithmo-Staticetea in the south-east of Italy. The revision is based on a data set of 225 relevés consisting of new and original phytosociological relevés (66) and others already published. The data matrix was classified with the use of flexible beta clustering. Indicator species analysis was employed to identify the indicator species of the main clusters of relevés. Results were interpreted from a syntaxonomic point of view. Non-metric multidimensional scaling ordination was performed in order to visualize the floristic relationships among associations. Rocky coastal vegetation of the Crithmo-Staticetea class in the south-east of Italy is represented by two orders, Crithmo maritimi-Staticetalia and Helichrysetalia italici. The first one includes two alliances, Crithmo-Staticion and Limonion anfracti-cancellati, with four associations and one, respectively. However, since they rely on very few character species, the floristic and syntaxonomic relationships between these two alliances need to be deepened by further investigations, involving a larger data set and investigation area. The second order, Helichrysetalia italici, includes two associations, well differentiated by their ecology, structure and floristic composition. For this reason, they were classified within two different alliances, Anthyllidion barbae-jovis and Helichrysion litorei. The Helichrysion litorei is here validated.

Algal assemblages in springs of different lithologies (ophiolites vs. limestone) of the Konjuh Mountain (Bosnia And Herzegovina)

The biodiversity of algal communities and environmental conditions were investigated in the springs of Mt. Konjuh. The assemblages of 20 springs emerging from different lithologies (limestones and ophiolites, respectively) comprised 234 algal taxa. Diatoms and cyanobacteria were the most species-rich groups. The most common alkaliphilic, circumneutral, and eutraphentic diatoms were represented by the genera Gomphonema, Nitzschia, Navicula, Cymbella, and Achnanthidium, and by the cyanobacterial genus Phormidium. Hierarchical clustering and SIMPROF analysis based on relative algal abundance clustered springs into six groups, separating them mainly according to spring type and lithology. Indicator species for groups and springs on different lithological substrata were singled out, revealing 33 taxa with preferences for ophiolites, and 20 taxa with preferences for carbonates. The values of the Shannon-Wiener diversity index were moderately high per spring location, and similar for the two groups of springs on different lithologies. A higher similarity in species composition was noted between springs on ophiolites and limestones than between springs on ophiolites and other types of siliceous substrata. The present study suggests that algal assemblages in springs emerging from ophiolites, even those made up by a preponderance of silicates, should be analyzed separately from those related to springs on other siliceous substrata. The results obtained showed that most of the springs studied are affected by anthropogenic impacts and morphological alterations leading to the dominance of highly competitive meso- and eutraphentic algal species, thus emphasizing the importance of further investigation and conservation of these habitats.

Responses of phytochelatin and proline-related genes expression associated with heavy metal stress in Solanum lycopersicum

The expression of stress related-genes against adverse environmental conditions has essential importance for plants. This study, using RT-qPCR, determined the expression of P5CS and PCS genes to investigate their roles in the leaves of tomato plants grown under heavy metal conditions. The expression of the PCS1 gene is significantly induced under such conditions. Transcript expression of P5CS1, a gene responsible for proline synthesis, changed depending on heavy metal doses; treatments of Cu (20 and 50 ppm), Cd and Pb (10 and 20 ppm) remarkably increased P5CS1 expression. However, the P5CS1 gene expression at 10 ppm dose of Cu and 50 ppm doses of Pb and Cd was not significantly different from that in control plants. The metal-chelating potency of the extract of tomato leaves exposed to Pb and Cd was higher than that of untreated plants. The proline content as assessed in the leaves of stressed plants was significantly increased by applications of 10 and 20 ppm of Cd and Pb, and high doses of Cu. In addition, the results showed that the proline content had a positive correlation with the P5CS1 gene expression in tomato leaves under application of these tree heavy metals and that there was a positive relation between the PCS1 gene expression and metal-chelating ability of Cd-stressed plants.