The spatial analysis of distribution and quantity of Hyphantria cunea Drury, 1973 across Ukraine since 1952 till 2016 regarding the values of annual absolute temperatures of ground air is performed using the Gis-technologies. The long-term pest dissemination data (Annual reports…, 1951–1985; Surveys of the distribution of quarantine pests ..., 1986–2017) and meteorological information (Meteorological Yearbooks of air temperature the surface layer of the atmosphere in Ukraine for the period 1951-2016; Branch State of the Hydrometeorological Service at the Central Geophysical Observatory of the Ministry for Emergencies) were used in the present research. The values of boundary negative temperatures of winter diapause of Hyphantria cunea, that unable the development of species’ subsequent generation, are received. Data analyses suggests almost complete elimination of winter diapausing individuals of White American Butterfly (especially pupae) under the air temperature of −32°С. Because of arising questions on the time of action of absolute minimal air temperatures, it is necessary to ascertain the boundary negative temperatures of winter diapause for White American Butterfly. It is also necessary to perform the more detailed research of a corresponding biological material with application to the freezing technics, giving temperature up to −50°С, with the subsequent analysis of the received results by the punched-analysis.
