scholarly journals Comments on Dodson et al., A Technical Comparison of Evaluating Asbestos Concentration by Phase Contrast Microscopy (PCM), Scanning Electron Microscopy (SEM), and Analytical Transmission Electron Microscopy (ATEM) as Illustrated from Data Generated from a Case Report

2008 ◽  
Vol 20 (12) ◽  
pp. 1113-1114 ◽  
Author(s):  
Victor L. Roggli ◽  
Thomas A. Sporn ◽  
Kelly J. Butnor
1976 ◽  
Vol 20 (3) ◽  
pp. 699-705
Author(s):  
G.M. Gray ◽  
H.J. Yardley

A suspension of epidermal cells obtained from pig tail skin by trypsinization was subjected to high liquid-shear forces in a French press. The material issuing from the press was examined by phase-contrast microscopy, transmission electron microscopy and scanning electron microscopy. The cytoskeleton of tonofibrils retained the shape of cell fragments, and subcellular organelles remained enmeshed in the network of tonofibrils. Examination of some cell fragments by scanning electron microscopy revealed the internal organization of the tonofibrils. The relevance of these findings to the problem of isolating subcellular fractions from epidermis is discussed.


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