Practice for Sampling and Counting Airborne Fibers, Including Asbestos Fibers, in the Workplace, by Phase Contrast Microscopy (with an Option of Transmission Electron Microscopy)

2020 ◽  
Author(s):  
2012 ◽  
Vol 61 (2) ◽  
pp. 129-135 ◽  
Author(s):  
MONIKA STANISZEWSKA ◽  
MAŁGORZATA BONDARYK ◽  
KATARZYNA SIENNICKA ◽  
WIESŁAW KURZĄTKOWSKI

A modified method of glutaraldeyde-osmium tetroxide fixation was adjusted to characterize the ultrastructure of Candida albicans pleomorphic forms, using phase-contrast microscopy, scanning electron microscopy and transmission electron microscopy. The discovered morphological criteria defining the individual morphotypes are discussed in terms of mycological and histopathological diagnostics of candidiasis. The relations are discussed between fungal pleomorphism, virulence and susceptibility of different morphotypes to fungicides.


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