Characterization of the interactions between immobilized parathion and the corresponding recombinant scfv antibody using a piezoelectric biosensor

1998 ◽  
Vol 10 (4) ◽  
pp. 363-374 ◽  
Author(s):  
Jirí Horáček ◽  
Stephen D. Garrett ◽  
Petr Skládal ◽  
Michael R. A. Morgan
Keyword(s):  
Scfv Antibody ◽  
Recombinant Scfv

Characterization of a single-chain variable fragment (scFv) antibody directed against the human asialoglycoprotein receptor

2006 ◽  
Vol 44 (2) ◽  
pp. 65 ◽  
Author(s):  
Guanxin Shen ◽  
Limin Cao ◽  
Yinchang Zhu ◽  
Weiyu Wang ◽  
Xiaorong Zhao ◽  
...  
Keyword(s):  
Asialoglycoprotein Receptor ◽  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain

scholarly journals Developing and characterization of single chain variable fragment (scFv) antibody against frizzled 7 (Fzd7) receptor

Bioengineered ◽  
2016 ◽  
Vol 8 (5) ◽  
pp. 501-510 ◽  
Author(s):  
Hamid Nickho ◽  
Vahid Younesi ◽  
Leili Aghebati-Maleki ◽  
Morteza Motallebnezhad ◽  
Jafar Majidi Zolbanin ◽  
...  
Keyword(s):  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain

scholarly journals Generation and characterization of a human single-chain fragment variable (scFv) antibody against cytosine deaminase from Yeast

2008 ◽  
Vol 8 (1) ◽  
pp. 68 ◽  
Author(s):  
Alessandra Mallano ◽  
Silvia Zamboni ◽  
Giulia Carpinelli ◽  
Filippo Santoro ◽  
Michela Flego ◽  
...  
Keyword(s):  
Cytosine Deaminase ◽  
Scfv Antibody ◽  
Single Chain ◽  
Chain Fragment ◽  
Single Chain Fragment Variable ◽  
Single Chain Fragment

scholarly journals Selection and characterization of scFv antibody against nucleocapsid protein of Porcine reproductive and respiratory syndrome virus

2020 ◽  
Vol 89 (1) ◽  
pp. 39-45
Author(s):  
Magdalena Krasna ◽  
Vladimír Celer
Keyword(s):  
Infectious Agent ◽  
Scfv Antibody ◽  
Respiratory Syndrome Virus ◽  
Single Chain ◽  
Single Chain Antibody ◽  
N Protein ◽  
E Coli ◽  
Metal Affinity ◽  
Binding Epitope

Porcine reproductive and respiratory syndrome virus (PRRSV) is a widespread infectious agent in pigs. Nucleocapsid (N) protein of PRRSV has been identified as the most immunodominant viral protein. The main goal of the work was the selection and characterization of a single-chain antibody fragments (scFv) antibody specific to the N protein. Specific scFv antibody clone D5 was selected from the Tomlinson phagemid library and purified by immobilized metal affinity chromatography from the periplasmatic space of E. coli cells. The antibody was then characterized by sequencing and the ability to recognize the native virus N protein by Western blot and competitive ELISA. Pepscan analysis identified the position of the binding epitope between amino acids 62–84 of the N protein. Our study could help to improve the diagnostics and prevention of PRRSV in Central Europe.

Construction, expression, and characterization of a single-chain variable fragment (ScFv) antibody targeting to theencephalomyocarditis virus

2018 ◽  
Vol 90 (7) ◽  
pp. 1184-1191 ◽  
Author(s):  
Haixia Zhang ◽  
Xinglong Wang ◽  
Xiangrong Li ◽  
Zhongren Ma ◽  
Ruofei Feng
Keyword(s):  
Scfv Antibody ◽  
Single Chain Variable Fragment ◽  
Single Chain ◽  
Antibody Targeting

Production and Characterization of Recombinant scFv Against Digoxin by Phage Display Technology

2013 ◽  
Vol 32 (3) ◽  
pp. 172-179 ◽  
Author(s):  
Behruz Alirezapour ◽  
Masoumeh Rajabibazl ◽  
Mohhamad Javad Rasaee ◽  
Kobra Omidfar
Keyword(s):  
Phage Display ◽  
Phage Display Technology ◽  
Recombinant Scfv ◽  
Display Technology
Sign in / Sign up

Export Citation Format

Share Document