Effects of autodisseminatedMetarhizium guizhouensePSUM02 on mating propensity and mating competitiveness ofBactrocera cucurbitae(Diptera: Tephritidae)

2015 ◽  
Vol 25 (6) ◽  
pp. 629-644 ◽  
Author(s):  
N. Thaochan ◽  
A. Ngampongsai
Keyword(s):  
Mating Competitiveness ◽  
Mating Propensity

scholarly journals Daya Saing Kawin Nyamuk Jantan Steril (Culex quinquefasciatus) Skala Laboratorium: Studi Awal Penggunaan Teknik Serangga Mandul dalam Pengendalian Vektor Filariasis Limfatik di Kota Pekalongan

2018 ◽  
pp. 65-72
Author(s):  
Tri Ramadhani ◽  
Upik Kusuma Hadi ◽  
Susi Soviana ◽  
Zubaidah Irawati ◽  
Sunaryo Sunaryo
Keyword(s):  
Gamma Irradiation ◽  
Experimental Research ◽  
Culex Quinquefasciatus ◽  
Wild Population ◽  
Optimum Dose ◽  
Normal Female ◽  
Wild Type ◽  
Mating Competitiveness ◽  
The Mean ◽  
Male Mosquitoes

Culex quinquefasciatus is the main vector of limfatic filariasis in Pekalongan City. Sterile Insect Tehnique could be an alternative vector control efforts to eliminate filariasis. The success of this technique is depend on the ability of laboratory-reared sterile males with the wild-type females. Indicator of SIT Aplication is determined by the value of the mating competitiveness and sterility to Culex quinquefasciatus (Diptera:Culicidae). The design of the research is an experimental. Gamma irradiation on the pupae (age . 15 hours) with the  doses of 0 Gy, 60 Gy, 65 Gy,70 Gy, 75 Gy and 80 Gy in BATAN Jakarta.  Male mosquitoes which emerged from the pupa then matting with a normal female. This research observed the mean  of females laying eggs ,fecundity, fertility and  mating competitiveness. This experimental research was conducted in the laboratory and the data were analyzed by ANOVA.The result showed that irradiation at the trial doses had an effect on fertility of Culex quinquefasciatus, but not  had significant effect on  fecundity and mating competitiveness . A dose of 70 Gy is the optimum dose with a fertility rate of 1.8% (sterility 98.2%) and C indexs 0,568 can be recommended for futher  semi field assays. The number of sterile males were six times compared with the wild population to increase the chances of  mating with wild-type females.

scholarly journals Investigation of the influence of a glutathione S-transferase metabolic resistance to pyrethroids/DDT on mating competitiveness in males of the African malaria vector, Anopheles funestus

2019 ◽  
Vol 4 ◽  
pp. 13 ◽  
Author(s):  
Magellan Tchouakui ◽  
Billy Tene Fossog ◽  
Brigitte Vanessa Ngannang ◽  
Doumani Djonabaye ◽  
Williams Tchapga ◽  
...  
Keyword(s):  
Natural Populations ◽  
Anopheles Funestus ◽  
Resistance Mechanisms ◽  
Malaria Vectors ◽  
Glutathione S Transferase ◽  
Metabolic Resistance ◽  
Mating Competitiveness ◽  
Metabolic Marker ◽  
Susceptible Allele ◽  
Permanet 3.0

Background: Metabolic resistance is a serious challenge to current insecticide-based interventions. The extent to which it affects natural populations of mosquitoes including their reproduction ability remains uncharacterised. Here, we investigated the potential impact of the glutathione S-transferase L119F-GSTe2 resistance on the mating competitiveness of male Anopheles funestus, in Cameroon. Methods: Swarms and indoor resting collections took place in March, 2018 in Tibati, Cameroon. WHO tube and cone assays were performed on F1 mosquitoes from indoor collected females to assess the susceptibility profile of malaria vectors. Mosquitoes mated and unmated males collected in the swarms were genotyped for the L119F metabolic marker to assess its association with mating male competitiveness. Results: Susceptibility and synergist assays, showed that this population was multiple resistant to pyrethroids, DDT and carbamates, likely driven by metabolic resistance mechanisms. Cone assays revealed a reduced efficacy of standard pyrethroid-nets (Olyset and PermaNet 2.0) with low mortality (<25%) whereas synergist PBO-Nets (Olyset Plus and PermaNet 3.0) retained greater efficacy with higher mortality (>80%). The L119F-GSTe2 mutation, conferring pyrethroid/DDT resistance, was detected in this An. funestus population at a frequency of 28.8%. In addition, a total of 15 mating swarms were identified and 21 An. funestus couples were isolated from those swarms.  A comparative genotyping of the L119F-GSTe2 mutation between mated and unmated males revealed that heterozygote males 119L/F-RS were less able to mate than homozygote susceptible (OR=7.2, P<0.0001). Surprisingly, heterozygote mosquitoes were also less able to mate than homozygote resistant (OR=4.2, P=0.010) suggesting the presence of a heterozygote disadvantage effect. Overall, mosquitoes bearing the L119-S susceptible allele were significantly more able to mate than those with 119F-R resistant allele (OR=2.1, P=0.03). Conclusion: This study provides preliminary evidences that metabolic resistance potentially exerts a fitness cost on mating competiveness in resistant mosquitoes.

scholarly journals Fluorescent markers rhodamine B and uranine for Anopheles gambiae adults and matings

2020 ◽  
Author(s):  
Erica i Aviles ◽  
Rachel D Rotenberry ◽  
C Matilda Collins ◽  
Ellen M Dotson ◽  
Mark Q Benedict
Keyword(s):  
Anopheles Gambiae ◽  
Rhodamine B ◽  
Seminal Fluid ◽  
Adult Longevity ◽  
Male Mating ◽  
Potential Contribution ◽  
Laboratory Studies ◽  
Mating Competitiveness ◽  
Fluorescent Markers ◽  
Auto Fluorescence

Abstract Background Marking mosquitoes is vital for mark-release-recapture and many laboratory studies, but their small size precludes the use of methods that are available for larger animals such as unique identifier tags and radio devices. Fluorescent dust is the most commonly used method to distinguish released individuals from the wild population. Numerous colours and combinations can be used, however, dust sometimes affects longevity and behaviour so alternatives that do not have these effects would contribute substantially. Rhodamine B has previously been demonstrated to be useful for marking adult Aedes aegypti males when added to the sugar meal. Unlike dust, this also marked the seminal fluid making it possible to detect matings by marked males in the spermatheca of females. Here, marking of Anopheles gambiae sensu stricto with rhodamine B and uranine was performed to estimate their potential contribution. Methods Two fluorescent markers, rhodamine B and uranine, were dissolved in sugar water and fed to adult An. gambiae. Concentrations that are useful for marking individuals and seminal fluid were determined. The effects on adult longevity, the durability of the marking and detection of the marker in mated females was determined. Male mating competitiveness was also evaluated.Results Rhodamine B marking in adults is detectable for at least three weeks, however uranine marking declines with time and at low doses can be confused with auto-fluorescence. Both can be used for marking seminal fluid which can be detected in females mated by marked males, but, again, at low concentrations uranine-marking is more easily confused with the natural fluorescence of seminal fluid. Neither dye affected mating competitiveness.Conclusions Both markers tested could be useful for field and laboratory studies. Their use has substantial potential to contribute to a greater understanding of the bio-ecology of this important malaria vector. Rhodamine B has the advantage that it appears to be permanent and is less easily confused with auto-fluorescence. The primary limitation of both methods is that sugar feeding is necessary for marking and adults must be held for at least 2 nights to ensure all individuals are marked whereas dusts provide immediate and thorough marking.

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