scholarly journals Daya Saing Kawin Nyamuk Jantan Steril (Culex quinquefasciatus) Skala Laboratorium: Studi Awal Penggunaan Teknik Serangga Mandul dalam Pengendalian Vektor Filariasis Limfatik di Kota Pekalongan

2018 ◽  
pp. 65-72
Author(s):  
Tri Ramadhani ◽  
Upik Kusuma Hadi ◽  
Susi Soviana ◽  
Zubaidah Irawati ◽  
Sunaryo Sunaryo
Keyword(s):  
Gamma Irradiation ◽  
Experimental Research ◽  
Culex Quinquefasciatus ◽  
Wild Population ◽  
Optimum Dose ◽  
Normal Female ◽  
Wild Type ◽  
Mating Competitiveness ◽  
The Mean ◽  
Male Mosquitoes

Culex quinquefasciatus is the main vector of limfatic filariasis in Pekalongan City. Sterile Insect Tehnique could be an alternative vector control efforts to eliminate filariasis. The success of this technique is depend on the ability of laboratory-reared sterile males with the wild-type females. Indicator of SIT Aplication is determined by the value of the mating competitiveness and sterility to Culex quinquefasciatus (Diptera:Culicidae). The design of the research is an experimental. Gamma irradiation on the pupae (age . 15 hours) with the  doses of 0 Gy, 60 Gy, 65 Gy,70 Gy, 75 Gy and 80 Gy in BATAN Jakarta.  Male mosquitoes which emerged from the pupa then matting with a normal female. This research observed the mean  of females laying eggs ,fecundity, fertility and  mating competitiveness. This experimental research was conducted in the laboratory and the data were analyzed by ANOVA.The result showed that irradiation at the trial doses had an effect on fertility of Culex quinquefasciatus, but not  had significant effect on  fecundity and mating competitiveness . A dose of 70 Gy is the optimum dose with a fertility rate of 1.8% (sterility 98.2%) and C indexs 0,568 can be recommended for futher  semi field assays. The number of sterile males were six times compared with the wild population to increase the chances of  mating with wild-type females.

scholarly journals Effects of Gamma Irradiation Against Mating Competitiveness of Male Culex quinquefasciatus (Diptera:Culicidae) in Laboratory

2019 ◽  
Author(s):  
T Ramadhani ◽  
UK Hadi ◽  
S Soviana ◽  
Z Irawati ◽  
A Rahayu ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Culex Quinquefasciatus ◽  
Optimum Dose ◽  
Normal Female ◽  
Sterile Male ◽  
Wild Type ◽  
Mating Competitiveness ◽  
The Mean ◽  
Gamma Irradiated ◽  
One Way Anova

ABSTRACTCulex quinquefasciatus is the main vector of lymphatic filariasis in Pekalongan City. Sterile Insect Tehnique (SIT) could be complementary vector control for filariasis. The key success of the technique depend on the ability of laboratory-reared sterile males with the wild-type females.The aim of the research was to determine the mating competitiveness, the fecundity and the fertility of sterile male Culex quinquefasciatus. The pupae of Cx. quinquefasciatus were gamma irradiated at the doses of 60Gy, 70Gy, and 80Gy, while unirradiated pupae were prepared as control. The mosquitoes emerging from the irradiated pupae could mate with a normal female in the cages. It were observed for the mean female laying eggs, the fecundity, the fertility and the mating competitiveness. The data were analyzed by one way ANOVA. The result showed that the irradiated Cx. quinquefasciatus at the doses tested did not affect on the fecundity and the mating competitiveness, but the fertility was disturbed (sterile). A dose of 70 Gy was the optimum dose or a fertility rate of 1.8% (98.2% sterile), and the value of competitiveness (C index) was 0.568. Based on the result, the irradiated Cx. quinquefasciatus can be recommended for semifield application.

scholarly journals Effects of Gamma Irradiation on Mating Competitiveness of Male Culex quinquefasciatus (Diptera:Culicidae) in Laboratory

2020 ◽  
Vol 46 (3) ◽  
pp. 155
Author(s):  
T. Ramadhani ◽  
U.K. Hadi ◽  
S. Soviana ◽  
Z. Irawati ◽  
A. Rahayu ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Culex Quinquefasciatus ◽  
Mating Competitiveness

Organophosphorus insecticide resistance in a new strain of Culex quinquefasciatus (Diptera: Culicidae) from Tanga, Tanzania

1993 ◽  
Vol 83 (1) ◽  
pp. 67-74 ◽  
Author(s):  
A. Khayrandish ◽  
R.J. Wood
Keyword(s):  
Insecticide Resistance ◽  
Culex Quinquefasciatus ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Laboratory Culture ◽  
Fourth Instar ◽  
Resistance Allele ◽  
Susceptible Control ◽  
The Mean

AbstractFourth instar larvae of a new strain of Culex quinquefasciatus Say from Tanzania (TANGA) were tested for insecticide resistance. Initially, the resistance ratio (RR) to chlorpyrifos was 41.8, to temephos 30.8, to propoxur 3.7. After 2–3 years of laboratory culture, resistance to chlorpyrifos and propoxur had declined (chlorpyrifos 5.7, 3.8; propoxur 1.9, permethrin 1.9). Significant synergism was found between s, s, s-tributyl trithiophosphate (DEF) and chlorphyrifos, reducing the RR from 8.0 to 2.5. Synergism between piperonyl butoxide and permethrin was less than in a susceptible control strain. Twelve esterase isozymes of different relative mobilities (Rm) on polyacrylamide gel electrophoresis were identified, ten of which remained when the strain was reinvestigated two years (approximately 32 generations) later. Null activity for all but one of these bands was observed in some larvae. Four esterase bands (Rm 0.25, 0.27, 0.31, 0.34, designated A2, A3, B2, B3) showed polymorphism in activity, with very intense bands in some larvae. The mean frequency of bands with activity greater than standard, declined as organophosphorus (OP) resistance declined, but resistance was unconnected with the frequency of nulls at these positions. In mass larval assays of in vitro sensitivity of acetylcholinesterase (AChE) to propoxur, the I50 exceeded 10x10−4M, compared with 0.1x10−4M in a reverted resistant strain (RANGOON). Single larvel assays revealed heterogeneity, which was interpreted on the basis of an AChE resistance allele (AceR) with a frequency of 0.23.

scholarly journals FK506-binding protein FklB is involved in biofilm formation through its peptidyl-prolyl isomerase activity

2020 ◽  
Author(s):  
Chrysoula Zografou ◽  
Maria Dimou ◽  
Panagiotis Katinakis
Keyword(s):  
Biofilm Formation ◽  
Negative Regulator ◽  
Cell Length ◽  
Wild Type ◽  
Prolyl Isomerase ◽  
Ppiase Activity ◽  
Peptidyl Prolyl Isomerase ◽  
Knock Out ◽  
E Coli ◽  
The Mean

AbstractFklB is a member of the FK506-binding proteins (FKBPs), a family that consists of five genes in Escherichia coli. Little is known about the physiological and functional role of FklB in bacterial movement. In the present study, FklB knock-out mutant ΔfklB presented an increased swarming and swimming motility and biofilm formation phenotype, suggesting that FklB is a negative regulator of these cellular processes. Complementation with Peptidyl-prolyl isomerase (PPIase)-deficient fklB gene (Y181A) revealed that the defects in biofilm formation were not restored by Y181A, indicating that PPIase activity of FklB is modulating biofilm formation in E. coli. The mean cell length of ΔfklB swarming cells was significantly smaller as compared to the wild-type BW25113. Furthermore, the mean cell length of swarming and swimming wild-type and ΔfklB cells overexpressing fklB or Y181A was considerably larger, suggesting that PPIase activity of FklB plays a role in cell elongation and/or cell division. A multi-copy suppression assay demonstrated that defects in motility and biofilm phenotype were compensated by overexpressing sets of PPIase-encoding genes. Taken together, our data represent the first report demonstrating the involvement of FklB in cellular functions of E. coli.

scholarly journals Methodology Appraisal of Experimental Research Studies in Mathematics Teaching

2019 ◽  
Vol 5 (1) ◽  
pp. 40-45
Author(s):  
Capote Paul John
Keyword(s):  
Learning Strategies ◽  
Experimental Research ◽  
Teaching And Learning ◽  
Research Problem ◽  
Effect Sizes ◽  
P Value ◽  
Experimental Teaching ◽  
Teaching And Learning Strategies ◽  
The Mean ◽  
Research Studies

This study made use of Gene Glass Effect Size formula to estimate the mean effect sizes of the eleven (11) reviewed experimental research studies on the effect of experimental and conventional teaching and learning strategies to the academic performance of students in mathematics. A validated Inclusion Criteria was utilized in the selection of studies and a modified Methodology Appraisal Checklist was employed in the analysis of elements of the research problem and research methodology. The mean effect sizes and variances of the reviewed studies vary across the elements of the research problem and methodology, an indication that teaching and learning strategies are dependent on the quality of methodology used by the researcher. This study stressed that there is no enough evidence to prove that experimental teaching and learning strategies are more effective than conventional pedagogies in improving learnings in math, as the analysis of variance (ANOVA) revealed a p-value of 0.982 (critical value=0.05).

scholarly journals How to tell about errors not boringly: methodological analysis of expounding the basis of data processing

2020 ◽  
Vol 2020 (2 (131)) ◽  
pp. 104-115
Author(s):  
Oksana Lozovenko ◽  
Yevgeny Sokolov
Keyword(s):  
Confidence Interval ◽  
Experimental Research ◽  
Basic Skills ◽  
A Priori ◽  
Teaching Experience ◽  
Measured Quantity ◽  
General View ◽  
True Value ◽  
Training Interval ◽  
The Mean

The authors continue to report about results they have obtained in the process of creating a special introductory one-semester Laboratory Physics course «Search for Physics laws». It is known that the teaching experience and the results of the performed tests show that most students do not acquire the basic skills for conducting an experimental research. This course was built on the basis of the algorithm of systematic construction of students’ skills for carrying out an experimental research. The authors have used Galperin’s stepwise teaching procedure which was developed on the assumption that learning any kind of knowledge involves different kinds of actions. The authors have analysed different ways of how to expound the basic ideas of data analysis, and shown their connection with the point, syncretic and training-interval paradigms. Action diagrams are provided for each type of expounding. As an example of using the training-interval paradigm for teaching first-year students of a technical university, a specially designed lab session is presented in the article. The topic of the session is “The concept of a confidence interval”. Laboratory Work 1 “The Buffon-de Morgan Experiment”. This lab session meets several important requirements: a) the number of computations is minimised; b) a directly measurable quantity is considered; c) students are provided with a “fulcrum” in the form of a priori known true value of a quantity. A general view on measuring physics quantities is summarised in four quite unexpected for students “unpleasant axioms”: 1) none of measured values coincides with the true value of a quantity; 2) the mean of measured values does not coincide with the true value of a quantity; 3) even if, by a lucky chance, one of measured values or the mean coincided with the true value of a quantity, we would never know about it; 4) a confidence interval catches the true value of a measured quantity only in 68% of cases. The authors claim that the presented lab lesson allows demonstrating the equity of these “axioms” clearly and vividly, and that the organised laboratory sessions in the new way are significantly more successful in improving students’ basic skills of error analysis than traditional laboratory sessions.

scholarly journals THE EFFECT OF SILENT WAY METHOD ON THE STUDENTS’ SPEAKING COMPETENCE AT THE TENTH GRADE OF SMAN 1 KONTUKOOWUNA

2020 ◽  
Vol 5 (1) ◽  
pp. 18
Author(s):  
Wa Sani ◽  
Rohmana Rohmana ◽  
Muhammad Khusnun Muhsin
Keyword(s):  
Experimental Research ◽  
Random Sampling ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Test Design ◽  
Paired Sample ◽  
Oral Test ◽  
Tenth Grade ◽  
Post Test ◽  
The Mean

The objective of the study was to find out whether or not the silent way method can significantly affect students’ speaking competence. The design of this study was a pre-experimental research design (one group pre-test post-test design). The researcher applied a simple random sampling technique. The class 10 IPA1 was taken as the sample of the study with 18 students. The instrument of this study was an oral test in pre-test and post-test. This research was conducted through the following procedures: giving a pre-test, applying treatments and giving a post-test. The data were analyzed by using paired sample t-test. Through the SPSS16.0 version. The result showed that the mean score on students’ speaking competence in a pre-test was 32.22 and in the post-test was 49.44. Hypothesis testing found that the value of sig (2 tailed) was 0.00 while the significant alpha value was 0.0. It could be concluded that the value of sig (2 tailed) was lower than the significant alpha value was (0.00<0.05). Therefore, H1 was accepted and H0 was rejected. So, the use of the silent way method had a significant on the students’ speaking competence at the tenth grade of SMAN 1 Kontu kowuna.

scholarly journals Old but Gold: The Use of Picture Cues to Teach Writing (An Experimental Research)

2018 ◽  
Vol 1 (1) ◽  
pp. 38
Author(s):  
Soviyah Soviyah ◽  
Yunia Purwaningtias
Keyword(s):  
Experimental Research ◽  
Junior High School ◽  
Control Group ◽  
Sample Population ◽  
Central Java ◽  
Significant Difference ◽  
Post Test ◽  
The Mean ◽  
And Control ◽  
Experimental Group

Pictures use in an educational setting fits the idiom: old but gold. There have been plenty of studies revealing how good the use of pictures in the classrooms is. Pictures have been long known to have the ability to help motivate, demonstrate, and instruct the students during a learning process. This study is a sort of complement to the phenomenon as it focuses on researching the use of pictures in a classroom. Specifically, it aims at finding out the effectiveness of the use of picture cues in teaching writing.The study belongs to experimental research involving two groups of experiment and control. The eighth-grade students of a private junior high school in Central Java Indonesia are chosen as the subject of the research during 2017/2018 academic year. Employing a sample population technique, the study involves a total number of 56 students who are equally divided into 28 students as the experimental group and 28 students as the control group. The experimental group is taught using picture cues while the control group is taught without picture cues. Pre-test and post-test are used as techniques to collect the data. As for the data analysis technique, it’s done quantitatively applying both descriptive and inferential statistics.The result of the research shows that there is a significant difference in the students’ writing ability between those taught using picture cues and those who are not. This can be seen from the difference in the mean score between them. The result of the pre-test shows that at the beginning, both experimental and control groups have slightly different ability in writing with the mean scores 51.32 and 47.86 respectively. Meanwhile, the result of the post-test indicates an obvious difference between them in which the experimental group gains 65.75 as its mean score and the control group gets 59.14. Furthermore, the application of Independent t-test calculation results in a score of 0.000, which is lower than 0.05. This means that the use of picture cues is effective. Based on these results, it can be concluded that the use of picture cues is effective to teach writing to students.

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