Gamma Irradiation of the European Chafer. 3. Longevity, Flight Behavior, and Mating Competitiveness of Sterilized Males, with Observations on Reproductive Biology123

1971 ◽  
Vol 64 (4) ◽  
pp. 883-886
Author(s):  
S. L. Chung ◽  
H. Tashiro ◽  
L. M. Massey
Keyword(s):  
Gamma Irradiation ◽  
Flight Behavior ◽  
Mating Competitiveness

scholarly journals Daya Saing Kawin Nyamuk Jantan Steril (Culex quinquefasciatus) Skala Laboratorium: Studi Awal Penggunaan Teknik Serangga Mandul dalam Pengendalian Vektor Filariasis Limfatik di Kota Pekalongan

2018 ◽  
pp. 65-72
Author(s):  
Tri Ramadhani ◽  
Upik Kusuma Hadi ◽  
Susi Soviana ◽  
Zubaidah Irawati ◽  
Sunaryo Sunaryo
Keyword(s):  
Gamma Irradiation ◽  
Experimental Research ◽  
Culex Quinquefasciatus ◽  
Wild Population ◽  
Optimum Dose ◽  
Normal Female ◽  
Wild Type ◽  
Mating Competitiveness ◽  
The Mean ◽  
Male Mosquitoes

Culex quinquefasciatus is the main vector of limfatic filariasis in Pekalongan City. Sterile Insect Tehnique could be an alternative vector control efforts to eliminate filariasis. The success of this technique is depend on the ability of laboratory-reared sterile males with the wild-type females. Indicator of SIT Aplication is determined by the value of the mating competitiveness and sterility to Culex quinquefasciatus (Diptera:Culicidae). The design of the research is an experimental. Gamma irradiation on the pupae (age . 15 hours) with the  doses of 0 Gy, 60 Gy, 65 Gy,70 Gy, 75 Gy and 80 Gy in BATAN Jakarta.  Male mosquitoes which emerged from the pupa then matting with a normal female. This research observed the mean  of females laying eggs ,fecundity, fertility and  mating competitiveness. This experimental research was conducted in the laboratory and the data were analyzed by ANOVA.The result showed that irradiation at the trial doses had an effect on fertility of Culex quinquefasciatus, but not  had significant effect on  fecundity and mating competitiveness . A dose of 70 Gy is the optimum dose with a fertility rate of 1.8% (sterility 98.2%) and C indexs 0,568 can be recommended for futher  semi field assays. The number of sterile males were six times compared with the wild population to increase the chances of  mating with wild-type females.

scholarly journals Effects of Gamma Irradiation Against Mating Competitiveness of Male Culex quinquefasciatus (Diptera:Culicidae) in Laboratory

2019 ◽  
Author(s):  
T Ramadhani ◽  
UK Hadi ◽  
S Soviana ◽  
Z Irawati ◽  
A Rahayu ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Culex Quinquefasciatus ◽  
Optimum Dose ◽  
Normal Female ◽  
Sterile Male ◽  
Wild Type ◽  
Mating Competitiveness ◽  
The Mean ◽  
Gamma Irradiated ◽  
One Way Anova

ABSTRACTCulex quinquefasciatus is the main vector of lymphatic filariasis in Pekalongan City. Sterile Insect Tehnique (SIT) could be complementary vector control for filariasis. The key success of the technique depend on the ability of laboratory-reared sterile males with the wild-type females.The aim of the research was to determine the mating competitiveness, the fecundity and the fertility of sterile male Culex quinquefasciatus. The pupae of Cx. quinquefasciatus were gamma irradiated at the doses of 60Gy, 70Gy, and 80Gy, while unirradiated pupae were prepared as control. The mosquitoes emerging from the irradiated pupae could mate with a normal female in the cages. It were observed for the mean female laying eggs, the fecundity, the fertility and the mating competitiveness. The data were analyzed by one way ANOVA. The result showed that the irradiated Cx. quinquefasciatus at the doses tested did not affect on the fecundity and the mating competitiveness, but the fertility was disturbed (sterile). A dose of 70 Gy was the optimum dose or a fertility rate of 1.8% (98.2% sterile), and the value of competitiveness (C index) was 0.568. Based on the result, the irradiated Cx. quinquefasciatus can be recommended for semifield application.

scholarly journals Effects of Gamma Irradiation on Mating Competitiveness of Male Culex quinquefasciatus (Diptera:Culicidae) in Laboratory

2020 ◽  
Vol 46 (3) ◽  
pp. 155
Author(s):  
T. Ramadhani ◽  
U.K. Hadi ◽  
S. Soviana ◽  
Z. Irawati ◽  
A. Rahayu ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Culex Quinquefasciatus ◽  
Mating Competitiveness

scholarly journals EFFECT OF GAMMA IRRADIATION ON THE QUALITY AND MATING COMPETITIVENES OF FRUIT FLIES Bactrocera dorsalis IN THE CAGE SCALE

2019 ◽  
Vol 18 (2) ◽  
pp. 160
Author(s):  
Indah Arastuti Nasution ◽  
Elvinasari Elvinasari ◽  
Dewi Hastuti
Keyword(s):  
Radiation Dose ◽  
Gamma Irradiation ◽  
Fruits And Vegetables ◽  
Fruit Fly ◽  
Bactrocera Dorsalis ◽  
Mating Competitiveness ◽  
Randomized Design ◽  
Adult Sex Ratio ◽  
Completely Randomized Design ◽  
Hatching Eggs

Bactrocera dorsalis is an important fruit fly attack fruits and vegetables in Indonesia. Sterile Insect Technique (SIT) is sterilize with gamma irradiation, released as many as 9 x wild population, ecpected to mate but didn’t produce offspring.  The research porpuse was to determine the effect of gamma irradiation on the quality and on the mating competitiveness in cage scale.  The experimental design was completely randomized design with one factor was gamma irradiation dosee at 6 levels were 0 (control), 30, 50, 70, 90 and 110 Gy as many as 4 replicates. The result that the level of the radiation dose had a significant on the competitiveness and quality included the percentage of pupae became adult adult, sex ratio, the percentage of fly ability, the number of eggs produced (fecundity) and percentage hatching eggs.  The quality decreased when  increasing in radiation dose.  The dose level didn’t affect the fecundity but affected the mating competitiveness in cage scale.  The value total of mating campetitivenes was highest at a dose of 70 Gy treatment were 0.76 (5:1:1) and 0.79 (10:1:1), followed by dose of 50 Gy were 0,41 (5:1:1) and 0,50 (10:1:1). 

Investigation of Developmental Stage/Age, Gamma Irradiation Dose, and Temperature in Sterilization of Male Aedes aegypti (Diptera: Culicidae) in a Sterile Insect Technique Program

2021 ◽  
Author(s):  
Beni Ernawan ◽  
Tjandra Anggraeni ◽  
Sri Yusmalinar ◽  
Intan Ahmad
Keyword(s):  
Aedes Aegypti ◽  
Gamma Irradiation ◽  
Sterile Insect Technique ◽  
Irradiation Dose ◽  
Developmental Stages ◽  
Pupal Stage ◽  
Standard Protocol ◽  
Mating Competitiveness ◽  
Sterile Insect Technique Program ◽  
High Level

Abstract The sterilization process using gamma irradiation is a crucial component in a program using sterile insect technique (SIT) to control Aedes aegypti. Unfortunately, there is no efficient standard protocol for sterilizing mosquitoes that can produce a high level of sterility while maintaining mating ability and longevity. Therefore, we conducted a study of the critical factors necessary to develop such a standard protocol. In this study, male Ae. aegypti pupae, as well as adults aged 1 d and 3 d, were irradiated using a Gamma-cell 220 irradiator doses of 0, 20, 40, 60, 70, 80, and 100 Gray (Gy). In addition, male Ae. aegypti in the pupal and adult stage aged 1 d were irradiated at a dose of 70 Gy at various temperatures. Changes in emergence rates, longevity, sterility, and mating competitiveness were recorded for each combination of parameters. Results showed that an increase of irradiation dose leads to a rise of induced sterility at all developmental stages, while simultaneously reducing emergence rate, survival, and mating competitiveness. Higher temperatures resulted in increased levels of sterility, reduced longevity, and did not affect the ability to mate. This study found that an irradiation dose of 70 Gy at a temperature between 20.00 and 22.30°C administered in the pupal stage induced a high level of sterility (around 98%), while maintaining mating competitiveness and longevity.

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