A novel SNP of TECPR2 gene associated with heat tolerance in Chinese cattle

With the inexorable rise of global temperature, heat stress deserves more and more attention in livestock agriculture. Previous studies have shown that the mechanistic target of rapamycin (MTOR) (NC_037343.1:c.2062G>C) gene contributes to the repair of DNA damage repair and is associated with the adaptation of camels in dry and hot environments. However, it is unknown whether this mutation is related to the heat tolerance of Chinese cattle. In this study, PCR and sequencing were used to type the mutation locus in 1030 individuals of 37 cattle breeds. The analysis results showed that the frequency of G allele of the locus gradually diminished from the northern group to the southern group of native Chinese cattle, whereas the frequency of the C allele showed an opposite pattern, displaying a significant geographical difference across native Chinese cattle breeds. Additionally, an analysis of the locus in Chinese indigenous cattle revealed that this SNP was significantly associated with mean annual temperature (T), relative humidity (RH) and temperature humidity index (THI) (p < 0.01), suggesting that cattle with C allele was distributed in regions with higher T, RH and THI. In conclusion, this study proved that the mutation of MTOR gene in Chinese cattle could be associated with the heat tolerance.

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Association of HSF1 Genetic Variation with Heat Tolerance in Chinese Cattle

Animals ◽

10.3390/ani9121027 ◽

2019 ◽

Vol 9 (12) ◽

pp. 1027

Author(s):

Yu Rong ◽

Mingfei Zeng ◽

Xiwen Guan ◽

Kaixing Qu ◽

Jianyong Liu ◽

...

Keyword(s):

Heat Tolerance ◽

North China ◽

Bos Indicus ◽

Heat Shock Factor 1 ◽

Heat Stress Response ◽

Chinese Cattle ◽

Specific Allele ◽

Native Cattle ◽

Humidity Index ◽

Hsf1 Gene

The heat shock factor 1 (HSF1) gene is a regulator of the heat stress response, maximizing HSP protein expression survival. In this research, we explored the frequency distribution of a missense mutation (NC_037341.1 g.616087A > G, rs135258919) in the HSF1 gene in Chinese cattle with amino acid substitution, valine to alanine. This mutation could be related to the heat tolerance in Bos indicus. A total of 941 individuals representing 35 Chinese native cattle breeds, combining pure taurine (Angus) and indicine cattle, were used to determine the genotypes of the mutation through PCR and partial DNA sequencing. The results showed significant differences in allele frequencies and their genotypes amongst Chinese cattle from different regions. Allele G or indicine-specific allele frequency diminished from south to north China, while allele A (genotype AA) or the taurine-specific allele had a contrary pattern, which agreed with the distribution of taurine and indicine cattle. According to the association analysis, the NC_037341.1 g.616087A > G (rs135258919) of the bovine HSF1 gene, annual temperature (T), relative humidity (RH), and the temperature humidity index (THI) (p < 0.01) were interrelated closely, which indicated that the NC_037341.1 g.616087A > G of the HSF1 gene is associated with heat tolerance in indicine cattle.

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A novel SNP of the ATP1A1 gene is associated with heat tolerance traits in dairy cows

Molecular Biology Reports ◽

10.1007/s11033-010-0080-8 ◽

2010 ◽

Vol 38 (1) ◽

pp. 83-88 ◽

Cited By ~ 40

Author(s):

Yanxin Liu ◽

Daqi Li ◽

Huixia Li ◽

Xuan Zhou ◽

Genlin Wang

Keyword(s):

Dairy Cows ◽

Heat Tolerance ◽

Novel Snp

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Four Novel SNPs of MYO1A Gene Associated with Heat-Tolerance in Chinese Cattle

Animals ◽

10.3390/ani9110964 ◽

2019 ◽

Vol 9 (11) ◽

pp. 964

Author(s):

Peng Jia ◽

Cuicui Cai ◽

Kaixing Qu ◽

Ningbo Chen ◽

Yutang Jia ◽

...

Keyword(s):

Heat Tolerance ◽

Bovine Genome ◽

Climatic Conditions ◽

Candidate Snps ◽

Phenotypic Effect ◽

Indigenous Cattle ◽

Synonymous Mutations ◽

Chinese Cattle ◽

Variation Database ◽

Intron Mutation

Based on the previous GWAS research related to bovine heat tolerance trait, this study aimed to explore the effect of myosin-1a (MYO1A) gene on bovine heat tolerance trait, and find the molecular markers related to the heat tolerance of Chinese cattle. In our study, four novel candidate SNPs highly conserved in B. indicus breeds but barely existed in B. taurus were identified in MYO1A gene according to Bovine Genome Variation Database and Selective Signatures (BGVD). PCR and DNA sequencing were used to genotype 1072 individuals including 34 Chinese indigenous cattle breeds as well as Angus and Indian zebu. Two synonymous mutations (rs208210464 and rs110123931), one missense mutation (rs209999142; Phe172Ser), and one intron mutation (rs135771836) were detected. The frequencies of mutant alleles of the four SNPs gradually increased from northern groups to southern groups of Chinese cattle, which was consistent with the distribution of various climatic conditions of China. Additionally, four SNPs were significantly associated with four climatic conditions including annual mean temperature (T), relative humidity (H), temperature-humidity index (THI), and average annual sunshine hours (100-cloudiness) (SR). Among these, rs209999142 and Hap 1/1 had better performance than others. Our results suggested that rs209999142 was associated with heat-tolerance trait and rs208210464, rs110123931, and rs135771836 showed high phenotypic effect on heat-tolerance trait because of the strong linkage with rs209999142. These SNPs could be used as candidates for marker-assisted selection (MAS) in cattle breeding.

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