Spectroscopy of RE3+ Centers in Fluorite Host Using High Resolution Optical Hole-Burning and Double Resonance Techniques

1996 ◽  
Vol 291 (1) ◽  
pp. 209-214
Author(s):  
Z. Hasan ◽  
Mikhail Solonenko ◽  
Levent Biyikli
Keyword(s):  
High Resolution ◽  
Double Resonance ◽  
Hole Burning

scholarly journals High-resolution EPR distance measurements on RNA and DNA with the non-covalent Ǵ spin label

2019 ◽  
Vol 48 (2) ◽  
pp. 924-933 ◽  
Author(s):  
Marcel Heinz ◽  
Nicole Erlenbach ◽  
Lukas S Stelzl ◽  
Grace Thierolf ◽  
Nilesh R Kamble ◽  
...  
Keyword(s):  
High Resolution ◽  
Nucleic Acids ◽  
Spin Label ◽  
Double Resonance ◽  
Conformational Dynamics ◽  
Md Simulations ◽  
Spin Labels ◽  
Abasic Site ◽  
Abasic Sites ◽  
Rna And Dna

Abstract Pulsed electron paramagnetic resonance (EPR) experiments, among them most prominently pulsed electron-electron double resonance experiments (PELDOR/DEER), resolve the conformational dynamics of nucleic acids with high resolution. The wide application of these powerful experiments is limited by the synthetic complexity of some of the best-performing spin labels. The recently developed $\bf\acute{G}$ (G-spin) label, an isoindoline-nitroxide derivative of guanine, can be incorporated non-covalently into DNA and RNA duplexes via Watson-Crick base pairing in an abasic site. We used PELDOR and molecular dynamics (MD) simulations to characterize $\bf\acute{G}$, obtaining excellent agreement between experiments and time traces calculated from MD simulations of RNA and DNA double helices with explicitly modeled $\bf\acute{G}$ bound in two abasic sites. The MD simulations reveal stable hydrogen bonds between the spin labels and the paired cytosines. The abasic sites do not significantly perturb the helical structure. $\bf\acute{G}$ remains rigidly bound to helical RNA and DNA. The distance distributions between the two bound $\bf\acute{G}$ labels are not substantially broadened by spin-label motions in the abasic site and agree well between experiment and MD. $\bf\acute{G}$ and similar non-covalently attached spin labels promise high-quality distance and orientation information, also of complexes of nucleic acids and proteins.

Spectral hole burning: High resolution optical spectroscopy and image storage

1988 ◽  
Vol 40-41 ◽  
pp. 823-824 ◽  
Author(s):  
Alois Renn ◽  
René Locher ◽  
Alfred Meixner ◽  
Urs P. Wild
Keyword(s):  
High Resolution ◽  
Optical Spectroscopy ◽  
Hole Burning ◽  
Spectral Hole Burning ◽  
Image Storage ◽  
Spectral Hole

High resolution double‐resonance spectroscopy on Rydberg states of CO

1993 ◽  
Vol 99 (8) ◽  
pp. 5701-5711 ◽  
Author(s):  
Marcel Drabbels ◽  
Johannes Heinze ◽  
J. J. ter Meulen ◽  
W. Leo Meerts
Keyword(s):  
High Resolution ◽  
Double Resonance ◽  
Rydberg States ◽  
Resonance Spectroscopy ◽  
Double Resonance Spectroscopy

scholarly journals High-resolution optical studies on C-phycocyanin via photochemical hole burning

1981 ◽  
Vol 103 (5) ◽  
pp. 1030-1035 ◽  
Author(s):  
J. Friedrich ◽  
H. Scheer ◽  
B. Zickendraht-Wendelstadt ◽  
D. Haarer
Keyword(s):  
High Resolution ◽  
Hole Burning ◽  
Optical Studies ◽  
Photochemical Hole Burning
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