Apparatus for nuclear-electron double resonance at 3300 gauss with moderately high resolution

1965 ◽  
Vol 42 (9) ◽  
pp. 675-676 ◽  
Author(s):  
J G Kenworthy ◽  
R E Richards
Keyword(s):  
High Resolution ◽  
Double Resonance

scholarly journals High-resolution EPR distance measurements on RNA and DNA with the non-covalent Ǵ spin label

2019 ◽  
Vol 48 (2) ◽  
pp. 924-933 ◽  
Author(s):  
Marcel Heinz ◽  
Nicole Erlenbach ◽  
Lukas S Stelzl ◽  
Grace Thierolf ◽  
Nilesh R Kamble ◽  
...  
Keyword(s):  
High Resolution ◽  
Nucleic Acids ◽  
Spin Label ◽  
Double Resonance ◽  
Conformational Dynamics ◽  
Md Simulations ◽  
Spin Labels ◽  
Abasic Site ◽  
Abasic Sites ◽  
Rna And Dna

Abstract Pulsed electron paramagnetic resonance (EPR) experiments, among them most prominently pulsed electron-electron double resonance experiments (PELDOR/DEER), resolve the conformational dynamics of nucleic acids with high resolution. The wide application of these powerful experiments is limited by the synthetic complexity of some of the best-performing spin labels. The recently developed $\bf\acute{G}$ (G-spin) label, an isoindoline-nitroxide derivative of guanine, can be incorporated non-covalently into DNA and RNA duplexes via Watson-Crick base pairing in an abasic site. We used PELDOR and molecular dynamics (MD) simulations to characterize $\bf\acute{G}$, obtaining excellent agreement between experiments and time traces calculated from MD simulations of RNA and DNA double helices with explicitly modeled $\bf\acute{G}$ bound in two abasic sites. The MD simulations reveal stable hydrogen bonds between the spin labels and the paired cytosines. The abasic sites do not significantly perturb the helical structure. $\bf\acute{G}$ remains rigidly bound to helical RNA and DNA. The distance distributions between the two bound $\bf\acute{G}$ labels are not substantially broadened by spin-label motions in the abasic site and agree well between experiment and MD. $\bf\acute{G}$ and similar non-covalently attached spin labels promise high-quality distance and orientation information, also of complexes of nucleic acids and proteins.

High resolution double‐resonance spectroscopy on Rydberg states of CO

1993 ◽  
Vol 99 (8) ◽  
pp. 5701-5711 ◽  
Author(s):  
Marcel Drabbels ◽  
Johannes Heinze ◽  
J. J. ter Meulen ◽  
W. Leo Meerts
Keyword(s):  
High Resolution ◽  
Double Resonance ◽  
Rydberg States ◽  
Resonance Spectroscopy ◽  
Double Resonance Spectroscopy

The distortion moment spectrum of GeH4: the microwave Q branch

1979 ◽  
Vol 57 (4) ◽  
pp. 593-600 ◽  
Author(s):  
R. H. Kagann ◽  
I. Ozier ◽  
G. A. McRae ◽  
M. C. L. Gerry
Keyword(s):  
High Resolution ◽  
Least Squares ◽  
Double Resonance ◽  
Complete Analysis ◽  
Resonance Spectroscopy ◽  
Centrifugal Distortion ◽  
Double Resonance Spectroscopy ◽  
The Absolute ◽  
Centrifugal Distortion Constants ◽  
Single Set

Ten pure rotational Q-branch transitions of the distortion moment spectrum of GeH4 have been measured between 9 and 23 GHz. They were observed in a conventional Stark modulated spectrometer modified to have a sensitivity of 1 × 10−11 cm−1. No Ge isotope splitting was observed in the spectrum. The spectrum was analysed in terms of a single set of six tensor centrifugal distortion constants DT, H4T, H6T, L4T, L6T, and L8T. The results were found to predict accurately transitions previously reported from infrared–microwave double resonance spectroscopy, and a complete analysis using both sets of data was carried out. The results are (in hertz): DT = 67 775.54 ± 0.86; H4T = −5.3827 ± 0.0055; H6T = 2.9693 ± 0.0019; L4T = (3.996 ± 0.088) × 10−4; L6T = −(4.122 ± 0.050) × 10−4; L8T = −(8.01 ± 0.14) × 10−4. The errors here are the standard deviations obtained from the least-squares analysis; estimates of the absolute errors are also given. For possible application in other high resolution studies, the constants have been used to calculate all tensor splittings for J ≤ 20.

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