HIGH PERFORMANCE LIQUID CHROMATOGRAPHY, TLC-DENSITOMETRY, AND FIRST-DERIVATIVE SPECTROPHOTOMETRY FOR SIMULTANEOUS DETERMINATION OF AMLODIPINE AND PERINDOPRIL IN BULK POWDER AND ITS TABLETS

2013 ◽  
Vol 36 (10) ◽  
pp. 1323-1339 ◽  
Author(s):  
Samia M. Gizawy ◽  
Loris I. Bebawy ◽  
Osama H. Abdelmageed ◽  
Mahmoud A. Omar ◽  
Sayed M. Deryea ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Derivative Spectrophotometry ◽  
First Derivative ◽  
Bulk Powder ◽  
First Derivative Spectrophotometry

scholarly journals High-performance liquid chromatography and derivative spectrophotometry for simultaneous determination of pravastatin and fenofibrate in the dosage form

2014 ◽  
Vol 64 (4) ◽  
pp. 433-446 ◽  
Author(s):  
Mohamed M. Hefnawy ◽  
Mostafa S. Mohamed ◽  
Mohammed A. Abounassif ◽  
Amer M. Alanazi ◽  
Gamal A. E. Mostafa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Particle Diameter ◽  
Second Order ◽  
Derivative Spectrophotometry ◽  
Order Derivative ◽  
Calibration Curves

Abstract High performance liquid chromatography (HPLC) and second-order derivative spectrophotometry have been used for simultaneous determination of pravastatin (PS) and fenofibrate (FF) in pharmaceutical formulations. HPLC separation was performed on a phenyl HYPERSIL C18 column (125 mm × 4.6 mm i.d., 5 μm particle diameter) in the isocratic mode using a mobile phase acetonitrile/0.1 % diethyl amine (50:50, V/V, pH 4.5) pumped at a flow rate of 1.0 mL min-1. Measurement was made at 240 nm. Both drugs were well resolved on the stationary phase, with retention times of 2.15 and 5.79 min for PS and FF, respectively. Calibration curves were linear (R = 0.999 for PS and 0.996 for FF) in the concentration range of 5-50 and 20-200 µg mL-1 for PS and FF, respectively. Pravastatin and fenofibrate were quantitated in combined preparations also using the second-order derivative response at 237.6 and 295.1 nm for PS and FF, respectively. Calibration curves were linear, with the correlation coefficient R = 0.999 for pravastatin and fenofibrate, in the concentration range of 5-20 and 3-20 µg mL-1 for PS and FF, respectively. Both methods were fully validated and compared, the results confirmed that they were highly suitable for their intended purpose.

scholarly journals Simple Spectrophotometric Methods for Determination of Tenofovir Fumarate and Emtricitabine in Bulk Powder and in Tablets

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Mohammad H. AbdelHay ◽  
Azza A. Gazy ◽  
Rasha A. Shaalan ◽  
Heba K. Ashour
Keyword(s):  
Simultaneous Determination ◽  
Analytical Performance ◽  
Derivative Spectrophotometry ◽  
Spectrophotometric Methods ◽  
First Derivative ◽  
Linearity Range ◽  
Bulk Powder ◽  
First Derivative Spectrophotometry

Two simple and selective methods were developed for the simultaneous determination of tenofovir fumarate (TEN) and emtricitabine (EMT) in combined tablets. The first method involves the application of first derivative spectrophotometry where the first derivative amplitudes were measured at 298.5 nm for determination of EMT in presence of TEN. The second method involves first derivative of ratio spectra spectrophotometry where the amplitudes at 251.5 nm have been used for quantitation of TEN in the presence of EMT. Different variables affecting each method were carefully investigated and optimized. Reliability and analytical performance of the proposed methods, including linearity, range, precision, accuracy, detection, and quantitation limits, were statistically validated. The methods were successfully applied for the determination of EMT and TEN in laboratory-prepared mixtures and in their combined tablets.

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