Simple Spectrophotometric Methods for Determination of Tenofovir Fumarate and Emtricitabine in Bulk Powder and in Tablets

Two simple and selective methods were developed for the simultaneous determination of tenofovir fumarate (TEN) and emtricitabine (EMT) in combined tablets. The first method involves the application of first derivative spectrophotometry where the first derivative amplitudes were measured at 298.5 nm for determination of EMT in presence of TEN. The second method involves first derivative of ratio spectra spectrophotometry where the amplitudes at 251.5 nm have been used for quantitation of TEN in the presence of EMT. Different variables affecting each method were carefully investigated and optimized. Reliability and analytical performance of the proposed methods, including linearity, range, precision, accuracy, detection, and quantitation limits, were statistically validated. The methods were successfully applied for the determination of EMT and TEN in laboratory-prepared mixtures and in their combined tablets.

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HIGH PERFORMANCE LIQUID CHROMATOGRAPHY, TLC-DENSITOMETRY, AND FIRST-DERIVATIVE SPECTROPHOTOMETRY FOR SIMULTANEOUS DETERMINATION OF AMLODIPINE AND PERINDOPRIL IN BULK POWDER AND ITS TABLETS

Journal of Liquid Chromatography &amp Related Technologies ◽

10.1080/10826076.2012.686141 ◽

2013 ◽

Vol 36 (10) ◽

pp. 1323-1339 ◽

Cited By ~ 2

Author(s):

Samia M. Gizawy ◽

Loris I. Bebawy ◽

Osama H. Abdelmageed ◽

Mahmoud A. Omar ◽

Sayed M. Deryea ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Simultaneous Determination ◽

High Performance ◽

Derivative Spectrophotometry ◽

First Derivative ◽

Bulk Powder ◽

First Derivative Spectrophotometry

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Liquid Chromatographic and Spectrophotometric Determination of Diflucortolone Valerate and Isoconazole Nitrate in Creams

Journal of AOAC International ◽

10.1093/jaoac/94.1.128 ◽

2011 ◽

Vol 94 (1) ◽

pp. 128-135 ◽

Cited By ~ 1

Author(s):

Elif Karacan ◽

Mehmet Gokhan Çaġlayan ◽

İsmail Murat Palabiyik ◽

Feyyaz Onur

Keyword(s):

Principal Component Regression ◽

Principal Component ◽

Derivative Spectrophotometry ◽

Data Matrix ◽

Concentration Data ◽

Spectrophotometric Methods ◽

First Derivative ◽

First Derivative Spectrophotometry ◽

Liquid Chromatographic

Abstract A new RP-LC method and two new spectrophotometric methods, principal component regression (PCR) and first derivative spectrophotometry, are proposed for simultaneous determination of diflucortolone valerate (DIF) and isoconazole nitrate (ISO) in cream formulations. An isocratic system consisting of an ACE® C18 column and a mobile phase composed of methanol–water (95+5, v/v) was used for the optimal chromatographic separation. In PCR, the concentration data matrix was prepared by using synthetic mixtures containing these drugs in methanol–water (3+1, v/v). The absorbance data matrix corresponding to the concentration data matrix was obtained by measuring the absorbances at 29 wavelengths in the range of 242–298 nm for DIF and ISO in the zero-order spectra of their combinations. In first derivative spectrophotometry, dA/dλ values were measured at 247.8 nm for DIF and at 240.2 nm for ISO in first derivative spectra of the solution of DIF and ISO in methanol–water (3+1, v/v). The linear ranges were 4.00–48.0 μg/mL for DIF and 50.0–400 μg/mL for ISO in the LC method, and 2.40–40.0 μg/mL for DIF and 60.0–260 μg/mL for ISO in the PCR and first derivative spectrophotometric methods. These methods were validated by analyzing synthetic mixtures. These three methods were successfully applied to two pharmaceutical cream preparations.

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