In vitro reproduction of three Limodorum species (Orchidaceae): impacts of scarification methods and nitrogen sources on mature seed germination and seedling development

2016 ◽  
Vol 151 (3) ◽  
pp. 419-428 ◽  
Author(s):  
S. Magrini ◽  
M. De Vitis
Keyword(s):  
Seed Germination ◽  
Nitrogen Sources ◽  
Seedling Development ◽  
Mature Seed

scholarly journals In vitro Embryo Morphogenesis and Micropropagation of Dendrobium aggregatum Roxb.

2014 ◽  
Vol 23 (2) ◽  
pp. 241-249 ◽  
Author(s):  
Mohammad Musharof Hossain
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Developmental Stages ◽  
Cell Mass ◽  
Multiple Shoot ◽  
Seedling Development ◽  
Ms Medium ◽  
Multiple Shoot Buds ◽  
Half Strength

In vitro embryo morphogenesis and micropropagation of Dendrobium aggregatum Roxb. were described. The gradual developmental stages of embryos to seedlings were traced out. Within two weeks of culture the cells of undifferented embryos underwent repeated aniclinal and periclinal division producing a compact, green parenchymatous cell mass called spherule that emerged out by rupturing the testa. The spherules subsequently differentiated into greenish protocorms were considered as typical seed germination. Germination occurred on both (MS and Phytamax (PM) medium but MS medium proved to be more efficient. The primary protocorms underwent profuse proliferation through production of secondary (2º) protocorms when transferred to different plant growth regulators (PGRs) supplemented MS; the medium fortified with 2.0 mg/l BAP and 1.0 mg/l NAA proved to be most effective for induction of 2º protocorms and seedling development. Multiple shoot buds (MSBs) were induced in pseudobulb segments of the in vitro grown seedlings when cultured on different PGRs supplemented media; and the maximum number of MSBs were obtained MS + 2.0 mg/l BAP + 0.5 mg/l picloram. The MSBs underwent elongation and then they rooted when they were transferred to half strength of MS + 0.5 mg/l IAA. The well rooted plantlets were finally transferred to outside natural environment with 80% survival. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17525 Plant Tissue Cult. & Biotech. 23(2): 241-249, 2013  (December)

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

Asymbiotic seed germination, in vitro seedling development, and greenhouse acclimatization of the threatened terrestrial orchid Bletia purpurea

2008 ◽  
Vol 94 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Daniela Dutra ◽  
Timothy R. Johnson ◽  
Philip J. Kauth ◽  
Scott L. Stewart ◽  
Michael E. Kane ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Development ◽  
Terrestrial Orchid ◽  
Asymbiotic Seed Germination

Asymbiotic seed germination and in vitro seedling development of the threatened orchid Hoffmannseggella cinnabarina

2012 ◽  
Vol 48 (5) ◽  
pp. 500-511 ◽  
Author(s):  
Rogério Mamoru Suzuki ◽  
Vania Carolina Moreira ◽  
Rosete Pescador ◽  
Wagner de Melo Ferreira
Keyword(s):  
Seed Germination ◽  
Seedling Development ◽  
Asymbiotic Seed Germination

scholarly journals ASYMBIOTIC GERMINATION OF CYPRIPEDIUM CALCEOLUS VAR. PARVIFLORUM (ORCHIDACEAE) IN VITRO.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 618d-618
Author(s):  
Chin-Chang Chu ◽  
Kenneth W. Mudge
Keyword(s):  
Seed Germination ◽  
Suspension Culture ◽  
Agar Medium ◽  
Germination Rate ◽  
Coconut Water ◽  
Mature Seed ◽  
Orchid Species ◽  
Cypripedium Calceolus ◽  
Chilling Treatment

Seeds from mature seed pods of Cypripedium calceolus var. parviflorum were germinated on 1/4 MSMO (Sigma) + 100ml/l coconut water + 1% sucrose +/- 8g/l agar (pH 6.0), and with or without prechilling at 5C for 8 weeks. Protocorm with apex (stage 3) was use as an index of germination. Seeds sown on agar medium withou chilling treatment resulted in a 40% germination rate in 120 days but the germination was very uneven. Seeds germinated on agar medium with prechilling developed more synchronously with 92% germination in 60 days (ie. about 120 days after sowing). Suspension culture of seeds without prechilling resulted in 85% germination after 90 days. The synchronization of seed germination in suspension culture was intermediate between that on agar with and without prechilling. Protocorms germinated in suspension culture appeared morphologically identical to those germinated on agar medium. All stage 3 protocorms developed further on the same agar medium in darkness. Both agar and suspension culture in media containing coconut water provided reliable seed germination methods for this orchid species.

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