Effects of plant growth regulators on in vitro seed germination and seedling development of Enhalus acoroides, a tropical seagrass

In order to estimate the best germination conditions of Encyclia aff. oncidioides seeds, five different media (Fast, Knudson C modified by Vajrabhaya, Murashige and Skoog, PB2 and modified Vacin and Went) with different concentrations of plant growth regulators such as benzyladenine (BA), naphthaleneacetic acid (NAA) and gibberellic acid (GA3) were tested. No beneficial effect was observed when BA and NAA were applied to the germination medium and GA3 inhibited germination. The effect of light, activated charcoal, coconut water and casein hydrolysate on seed germination was also studied. The growth rate of seedlings on three different media supplemented with activated charcoal and plant growth regulators was checked. The applied plant growth regulators had no beneficial effect on the further growth of seedlings. Fast and PB2 media with 0.2% activated charcoal proved to be the best for E. aff. oncidioides seed germination, seedling development and plantlet propagation.

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Assessment of Plant Growth Regulators and Carbon Sources on the Germination and Growth Process of Dandelion (Taraxacum officinale G.H. Weber ex Wiggers) under In Vitro Conditions

Horticulturae ◽

10.3390/horticulturae7110486 ◽

2021 ◽

Vol 7 (11) ◽

pp. 486

Author(s):

María Eugenia Martínez ◽

Lorena Jorquera ◽

Paola Poirrier ◽

Katy Díaz ◽

Rolando Chamy

Keyword(s):

Seed Germination ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Carbon Sources ◽

Optimal Growth ◽

Taraxacum Officinale ◽

Naphthaleneacetic Acid ◽

Low Carbon

There are several studies on the medicinal properties of dandelions (Taraxacum officinale), but few studies are aimed at understanding the in vitro germination process of this plant to improve its propagation. This research was focused on studying in vitro seed germination and development of seedlings under different carbon sources, glucose (GLU) or sucrose (SUC) and its concentrations (1.0–5.5%). Additionally, the effect of supplementation with plant growth regulators (PGRs) was studied, measuring the germination capacity, uncertainty and synchrony. Germination was promoted under low carbon source concentrations (≤2.3%), whereas higher concentrations (≥3.2%) had a detrimental effect on this process. GLU allowed the final germination percentages to be slightly better than SUC. Uniformity and synchrony values improved with the presence of PGRs. Results suggested that the best condition to assess T. officinale seed germination is in a medium containing GLU 2.3%, 0.5 mg/L 1-Naphthaleneacetic acid (NAA) and 0.5 mg/L 6-Benzylaminopurine acid (BAP). After germination, the best condition for optimal growth of T. officinale seedlings was 1.0% SUC supplemented with 0.225 mg/L of NAA and 3.0 mg/L of BAP for initial shoot development. The survival rate was 97% after greenhouse acclimatization. This new method of germination was implemented for the massive propagation of T. officinale for further medicinal studies.

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Effect of plant growth regulators on the growth of Orychophragmus violaceus plantlets in vitro

Planta Medica ◽

10.1055/s-0031-1282328 ◽

2011 ◽

Vol 77 (12) ◽

Cited By ~ 3

Author(s):

Y Wu ◽

W Xu

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Orychophragmus Violaceus

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Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽

10.3390/agronomy11020320 ◽

2021 ◽

Vol 11 (2) ◽

pp. 320

Author(s):

Nisar Ahmad Zahid ◽

Hawa Z.E. Jaafar ◽

Mansor Hakiman

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Zingiber Officinale ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Zingiber Officinale Roscoe ◽

Planting Materials ◽

Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

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IN VITRO RESPONSES OF AMERICAN CHESTNUT TO PLANT GROWTH REGULATORS IN CULTURE MEDIUM

Acta Horticulturae ◽

10.17660/actahortic.2009.844.31 ◽

2009 ◽

pp. 229-234 ◽

Cited By ~ 4

Author(s):

Guochen Yang ◽

Zhongge (Cindy) Lu ◽

T.M. Asante ◽

P.E. Read

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Culture Medium ◽

American Chestnut

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The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

Notulae Scientia Biologicae ◽

10.15835/nsb335931 ◽

2011 ◽

Vol 3 (3) ◽

pp. 97-100

Author(s):

Naimeh SHARIFMOGHADAM ◽

Abbas SAFARNEJAD ◽

Sayed Mohammad TABATABAEI

Keyword(s):

Tissue Culture ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Base Material ◽

Culture Technique ◽

Induction Medium ◽

Root Induction ◽

Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

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Effects of Plant Growth Regulators on In vitro Regeneration of Malaysian Indica rice (Oryza sativa L.) cv. MR219 by Shoot Apical Meristem

Asian Journal of Agricultural Research ◽

10.3923/ajar.2012.180.187 ◽

2012 ◽

Vol 6 (4) ◽

pp. 180-187 ◽

Cited By ~ 1

Author(s):

Lavanya Silvarajan ◽

Rosimah Nulit ◽

Faridah Qamaruz Za

Keyword(s):

Oryza Sativa ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Shoot Apical Meristem ◽

Apical Meristem ◽

Oryza Sativa L ◽

Indica Rice ◽

In Vitro Regeneration

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Sweet pepper seed responses to inoculation with microorganisms and coating with micronutrients, aminoacids and plant growth regulators

Scientia Agricola ◽

10.1590/s0103-90162009000300002 ◽

2009 ◽

Vol 66 (3) ◽

pp. 293-297 ◽

Cited By ~ 5

Author(s):

Kênia Almeida Diniz ◽

Paulo de Albuquerque Silva ◽

João Almir Oliveira ◽

José Renato Emiliorelli Evangelista

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Beauveria Bassiana ◽

Growth Regulators ◽

Metarhizium Anisopliae ◽

Trichoderma Viride ◽

Sweet Pepper ◽

Seedling Development ◽

Initial Development ◽

Antagonistic Microorganisms

Small sized seeds, such as the horticultural species, have limited quantities of reserves that can be balanced by coating then with essential nutrients for their initial development. In addition, inoculation of the seeds with microorganisms may protect the plants against phytopathogens, thus enhancing their growth. The present work had the objective of evaluate the physiological quality and seedling development of sweet pepper seeds and seedlings coated with several kind of films. Seeds were first coated with polymers and then with antagonistic microorganisms (Trichoderma viride, Trichoderma polysporhum, Trichoderma stromaticum, Beauveria bassiana, Metarhizium anisopliae), mycorrhizas, aminoacids, micronutrients and plant growth regulators. Evaluation was performed for percentage of germination and for seedling emergence, speed of emergence index, number of plants, dry mass of the aerial and root parts and height of the seedlings. Inoculation with Trichoderma viride increased the percentage and rate of the seedlings emergence Inoculation with Trichoderma viride, Metarhizium anisopliae and mycorrhizas promote better seedling development; seed microbiolization with microorganisms Trichoderma viride, T. polysporhum, T. stromaticum, Beauveria bassiana, Metarhizium anisopliae. Mycorrhizas mixture negatively affected seeds and seedling quality. Seed covering with plant growht regulator, at a 5 mL kg-1 dose increased the roots dry matter.

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