A rapid double-staining technique to improve seed viability testing in terrestrial orchids

Author(s):  
Sara Magrini ◽  
D. Barreca ◽  
L. Zucconi
1979 ◽  
Vol 24 (1) ◽  
pp. 7-14 ◽  
Author(s):  
D. Schweizer ◽  
P. Ambros ◽  
M. Andrle ◽  
A. Rett ◽  
W. Fiedler

1979 ◽  
Vol 13 (4) ◽  
pp. 309-310 ◽  
Author(s):  
J. Whitaker ◽  
Kathleen M. Dix

Cartilage and bone were differentiated using alcian blue and alizarin red S respectively. Anomalies of both cartilaginous and bony parts of the skeleton could be examined.


1985 ◽  
Vol 33 (12) ◽  
pp. 1247-1251 ◽  
Author(s):  
C Manigley ◽  
J Roth

We report the development of a new light-microscopic double-staining technique using colloidal gold as sole marker. The contrasting color to the red of colloidal gold is achieved by the application of photochemical silver reaction. The silver reaction, which is principally performed at the end of the first staining sequence, converts the red color of a gold-labeled reagent into black. This contrasts clearly with the red coloration that results from the second incubation sequence without silver reaction. For antigen double staining, the same protein A-gold complex can be used to provide the black and the red color, thus rendering the technique very economical. Alternatively, combination of protein A-gold immunolocalization and lectin-gold staining is possible, as is combined lectin-gold staining.


2013 ◽  
Vol 221 ◽  
pp. S217
Author(s):  
Céline Pique ◽  
Edward Marsden ◽  
Paul Quesada ◽  
Audrey Jocteur-Monrozier ◽  
Jean-Paul Briffaux

1993 ◽  
Vol 68 (3) ◽  
pp. 127-131 ◽  
Author(s):  
B. N. Achar ◽  
J. M. Bhandari ◽  
H. G. V. G. Urs

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