scholarly journals Control of biogas production process by enzymatic and fluorescent image analysis

2018 ◽  
Vol 32 (2) ◽  
pp. 366-375 ◽  
Author(s):  
Nora Dinova ◽  
Mihaela Belouhova ◽  
Irina Schneider ◽  
Jelyaz Rangelov ◽  
Yana Topalova
Científica ◽  
2016 ◽  
Vol 44 (3) ◽  
pp. 412 ◽  
Author(s):  
Rafael Marani Barbosa ◽  
Bruno Guilherme Torres Licursi Vieira ◽  
Francisco Guilhien Gomes-Junior ◽  
Roberval Daiton Vieira

2021 ◽  
Author(s):  
Mohammad Amui Khorshidi ◽  
Hossein Beiki ◽  
Mojtaba Kanvisi

Abstract Background: Since fossil fuels are limited and their burning is considered the main reason for environmental pollution, thinkers in the energy section are looking for a substitute for them. They have considered biogas as a potent replacement. Constant composition and volumetric rate, are ones of the challenges faced in term of using biogas. Therefore, in this study, a novel easily portable continuous bioreactor was designed and constructed to produce biogas at constant composition and volumetric rate, which is suitable for human uses. Sugar beet waste and anaerobic sludge were used as substrate and inoculum with an S/I ratio of 0.5 to 1, to produce biogas. Four parameters, i.e., hydraulic retention time (HRT), pH, biogas volume, and methane composition, were measured and compared.Results: The results of the mentioned reactor were compared with those of batch ones. The measurement revealed that the continuous reactor had a good performance on biogas purity and volumetric rate. The biogas contained about 53% methane. The suitable and preferable HRT and organic loading rate (OLR) were 18 days and 34.86 g VS/day. After the 18th day of operation, the biogas production process inside the continuous reactor was stable reaching about 411.2 ml STD/g VS per day.Conclusions: The reactor designed makes the biogas production process more manageable. Besides the production of the cumulative volume of biogas and constant methane percentage was achieved. As a result, the biogas produced is consumed daily, and a certain amount of gas is available every day. Since the percentage of gas produced is constant, it is possible to adjust the gas appliances with this amount of methane.


2019 ◽  
Author(s):  
Giorgio Cattoretti ◽  
Francesca Maria Bosisio ◽  
Lukas Marcelis ◽  
Maddalena Maria Bolognesi

Abstract Multiplexing, labeling for multiple immunostains the very same cell or tissue section in situ, is of considerable interest. The major obstacles to the diffusion of this technique are high costs in custom antibodies and instruments, low throughput, scarcity of specialized skills or facilities. We have validated and detail here a method based on common primary and secondary antibodies, diffusely available fluorescent image scanners and routinely processed tissue sections \(FFPE). It entails rounds of four-color indirect immunofluorescence, image acquisition and removal \(stripping) of the antibodies, before another stain is applied. The images are digitally registered and the autofluorescence is subtracted. Removal of antibodies is accomplished by disulphide cleavage. In excess of 50 different antibody stains can be applied to one single section from routinely fixed and embedded tissue. This method requires a modest investment in hardware and materials and uses freeware image analysis software.


1991 ◽  
Vol 26 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Krishna Nand ◽  
S. Sumithra Devi ◽  
Prema Viswanath ◽  
Somayaji Deepak ◽  
R. Sarada

2008 ◽  
Vol 14 (2) ◽  
pp. 201-207 ◽  
Author(s):  
Taeyoon Son ◽  
Byungkwan Han ◽  
Byungjo Jung ◽  
J. Stuart Nelson

2012 ◽  
Vol 84 (21) ◽  
pp. 9379-9387 ◽  
Author(s):  
Archana N. Rao ◽  
Christopher K. Rodesch ◽  
David W. Grainger

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