scholarly journals Molecular characterization and functional properties of avenin-like b gene TuALPb7As in Triticum urartu

2021 ◽  
Vol 35 (1) ◽  
pp. 276-282
Author(s):  
Dong Cao ◽  
Fahui Ye ◽  
Caixia Zhao ◽  
Jicheng Shen ◽  
Ruijuan Liu ◽  
...  
2009 ◽  
Vol 15 (1) ◽  
pp. 38-51 ◽  
Author(s):  
Igal Germanguz ◽  
Oshra Sedan ◽  
Naama Zeevi-Levin ◽  
Ronit Shtrichman ◽  
Efrat Barak ◽  
...  

1994 ◽  
Vol 131 (4) ◽  
pp. 385-390 ◽  
Author(s):  
Tadashi Kimura ◽  
Yoko Makino ◽  
Fumitaka Saji ◽  
Masahiko Takemura ◽  
Tomoko Inoue ◽  
...  

Kimura T, Makino Y, Saji F, Takemura M, Inoue T, Kikuchi T, Kubota Y, Azuma C, Nobunaga T, Tokugawa Y, Tanizawa O. Molecular characterization of a cloned human oxytocin receptor. Eur J Endocrinol 1994;131:385–90. ISSN 0804–4643 We describe here the binding and functional properties of a cloned human oxytocin receptor (OTR). We established a transient OTR expression system on COS-1 cells, which do not express vasopressin receptors. With the transfected cells and [3H]oxytocin, the dissociation constant (Kd) of OTR to oxytocin was 6.0 ±1.1 nmol/l; the binding properties of several oxytocin-related peptides were also examined. The functional properties of OTR were determined by an electrophysiological method, using a Xenopus laevis oocyte injected with in vitro transcribed OTR mRNA. These two methods showed that [Phe2,Orn8]vasotocin, a vasopressin agonist, was an OTR antagonist. A combination of these methods using cloned OTR cDNA is a novel and effective method for the investigation of oxytocin-related ligands. Tadashi Kimura, Department of Obstetrics and Gynecology, Osaka University Medical School, 2-2 Yamadaoka, Suita City, Osaka 565, Japan


1993 ◽  
Vol 27 (3) ◽  
pp. 275-284 ◽  
Author(s):  
Axel Steiger ◽  
Rainer Rupprecht ◽  
Dietmar Spengler ◽  
Jürgen Guldner ◽  
Ullrich Hemmeter ◽  
...  

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