Restriction endonuclease analysis of a Spodoptera litura nuclear polyhedrosis virus (NPV) isolate

IUBMB Life ◽  
1996 ◽  
Vol 39 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Rakha Das ◽  
Y. Durga Prasad
Keyword(s):  
Restriction Endonuclease ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Restriction Endonuclease Analysis ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Endonuclease Analysis

EFFECTS OF LARVAL AGE ON SUSCEPTIBILITY OF SPODOPTERA LITURA (LEPIDOPTERA: NOCTUIDAE) TO SPODOPTERA LITURA MULTIPLE NUCLEAR POLYHEDROSIS VIRUS

2000 ◽  
Vol 132 (3) ◽  
pp. 337-340 ◽  
Author(s):  
M.D. Monobrullah ◽  
Masao Nagata
Keyword(s):  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Actual Mechanism ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Time Required ◽  
Lepidoptera Noctuidae ◽  
Host Development

Susceptibility of many insects to baculoviruses decreases with host development (Hochberg 1991), whereas the time required for infected hosts to die increases with larval age (Boucias et al. 1980; Bucher and Turnock 1983; Smits and Vlak 1988). The actual mechanism of this phenomenon, however, has not been established in many cases. The present study was undertaken to investigate the effect of larval age on the susceptibility of Spodoptera litura Fabricius to nuclear polyhedrosis virus (NPV) through oral and intrahaemocoelic routes of infection.

scholarly journals Biological Test of Formulation of Subculture Helicovepa armigera Nuclear Polihedrosis Virus (HaNPV) on Mortality of Spodoptera litura Larvae infested to cabbage (Brassica oleracea Var. capitata Linn.) Plantation

2015 ◽  
Vol 2 (1) ◽  
pp. 646
Author(s):  
Desy Efriyani Anggraeny Nasution ◽  
Mia Miranti ◽  
Melanie .
Keyword(s):  
Brassica Oleracea ◽  
Helicoverpa Armigera ◽  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Insect Pests ◽  
Block Design ◽  
Economic Value ◽  
Helicoverpa Armígera ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

<p>Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV) is one of the biological agents that could be used for the development of organic farming in Indonesia. One of the organic agricultural food products which have high economic value is cabbage, HaNPV effectif to control the population of several species of insect pests, can be used instead of synthetic insecticides in agricultural development efforts of organic cabbage. HaNPV formulation can be used as a way to maintain the virulence properties of the virus and facilitate the storage and application. The preparation of subculture HaNPV formulations was in liquid, powders, cornstarch and talk carrier material. The design experimental was used randomized block design consists single factor (type HaNPV formulation consisting of powder formulations, liquid formulation, formulation in cornstarch, and talc formulation including water formulation without virus as a control), with eight replications. The results showed that all of the formulations of subculture HaNPV causing larvae mortality at range 97.4% -100%. The highest mortality of larvae occurred in cabbage plants are protected by HaNPV carrier liquid and talk preparations by 100% each. <br /><strong></strong></p><p><strong>Keywords</strong>: Formulations Helicoverpa armigera Nuclear Polyhedrosis Virus (HaNPV), subculture, Spodoptera litura, Brassica oleracea Var. capitata Linn.</p>

scholarly journals Uji Efektivitas Konsentrasi Spodoptera litura – Nuclear Polyhedrosis Virus (SlNPV) JTM 97C Formulasi Bubuk Terhadap Larva Spodoptera litura Fabricius (Lepidoptera: Noctuidae) Pada Tanaman Kedelai

2019 ◽  
Vol 2 (2) ◽  
pp. 46
Author(s):  
M Novel Ghufron Syahroni ◽  
Nanang Tri Haryadi
Keyword(s):  
Spodoptera Litura ◽  
Nuclear Polyhedrosis Virus ◽  
Larval Instar ◽  
Crop Failure ◽  
Randomized Design ◽  
Pathogenic Virus ◽  
Hot And Humid Climates ◽  
Polyhedrosis Virus ◽  
Soybean Plants ◽  
Nuclear Polyhedrosis

Spodoptera litura (armyworm) is an important leaf pest that is polyphagic and has a broad range of hosts. Spodoptera litura is the main pest in soybean plants and can cause up to 50% damage and often results in decreased productivity and even crop failure. Spodoptera litura is widespread in areas with hot and humid climates from the subtropics to the tropics. S. litura pests attack cultivation plants in the vegetative and generative phases. The prospect of insect pathogens as a substitute for chemical insecticides is quite good in terms of their effectiveness and impact on the environment. Nuclear Polyhedrosis Virus (NPV) is one of the Baculoviridae viruses that infect the larval stage. Spodoptera litura Nuclear Polyhedrosis Virus (SlNPV) is a pathogenic virus that infects armyworms. SlNPV JTM 97C is an effective isolate in controlling larvae of Spodoptera litura. The larvae used in this study were larval instar 4, instar 5, and instar 6. The study was conducted using a Completely Randomized Design (CRD) with 6 treatments and 3 replications. The treatments consisted of controls, 1 g / l, 2g / l, 3 g / l, 4 g / l, and 5 g / l. Each treatment was applied to instar 4, instar 5 and 6 instar larvae. The results showed that SlNPV JTM 97C affected mortality, stopped eating and pupa formation of instar 4, instar 5 larvae, but did not affect instar 6 larvae. The younger the larval instar the higher the pathogenicity response.

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