Investigation of genetic heterogeneity in wild isolates of Spodoptera frugiperda nuclear polyhedrosis virus by restriction endonuclease analysis of plaque-purified variants

Virology ◽  
1981 ◽  
Vol 112 (1) ◽  
pp. 190-197 ◽  
Author(s):  
John D. Knell ◽  
Max D. Summers
Keyword(s):  
Restriction Endonuclease ◽  
Genetic Heterogeneity ◽  
Spodoptera Frugiperda ◽  
Nuclear Polyhedrosis Virus ◽  
Restriction Endonuclease Analysis ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Endonuclease Analysis

scholarly journals A Nuclear-Polyhedrosis Virus of Spodoptera frugiperda Isolated in Puerto Rico.

1969 ◽  
Vol 63 (2) ◽  
pp. 162-169
Author(s):  
Goro Kuno
Keyword(s):  
Puerto Rico ◽  
Spodoptera Frugiperda ◽  
Inclusion Bodies ◽  
Nuclear Polyhedrosis Virus ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Heliothis Zea ◽  
Fourth Instar ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

A nuclear-polyhedrosis virus was isolated from a larva of Spodoptera frugiperda collected in Puerto Rico. The virus was found to be pathogenic to the larvae of Heliothis zea and of S. frugiperda but nonpathogenic to those of Diatraea saccharalis and of Galleria mellonella. LD50 for the fourth instar larvae of H. zea and S. frugiperda inoculated per os were 1.25 x 103 and 2.7 x 103 polyhedral inclusion bodies, respectively. The tissues infected included hemocytes, fat body, muscle, and epidermis. Furthermore, transovarian transmission of the virus was found in the inoculated individuals of S. frugiperda.

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