An update on the advancing high-throughput screening techniques for patch clamp-based ion channel screens: implications for drug discovery

2018 ◽  
Vol 13 (3) ◽  
pp. 269-277 ◽  
Author(s):  
Alison Obergrussberger ◽  
Tom A. Goetze ◽  
Nina Brinkwirth ◽  
Nadine Becker ◽  
Søren Friis ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Patch Clamp ◽  
Ion Channel ◽  
High Throughput ◽  
High Throughput Screening ◽  
Screening Techniques

Upscaling and Automation of Electrophysiology: Toward High Throughput Screening in Ion Channel Drug Discovery

2003 ◽  
Vol 9 (1) ◽  
pp. 49-58
Author(s):  
Margit Asmild ◽  
Nicholas Oswald ◽  
Karen M. Krzywkowski ◽  
Søren Friis ◽  
Rasmus B. Jacobsen ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Ion Channel ◽  
High Throughput ◽  
High Throughput Screening

INCUBATION TYPE PLANAR PATCH CLAMP AS A NEW POTENTIAL TECHNOLOGY FOR DEVELOPING NEURONAL NETWORK HIGH THROUGHPUT SCREENING DEVICES

2016 ◽  
Vol 28 (03) ◽  
pp. 1630002
Author(s):  
Tsuneo Urisu
Keyword(s):  
Patch Clamp ◽  
Ion Channel ◽  
High Throughput ◽  
Neuronal Network ◽  
High Throughput Screening ◽  
Hek293 Cells ◽  
Base Line ◽  
Channel Current ◽  
Trpv1 Channels ◽  
Probability Number

Ion-channel current recordings based on an incubation type planar patch clamp were first reported in 2008, using HEK293 cells expressed with TRPV1 channels and capsaicin as ligand molecules. At first the success probability (number of devices which normally worked/total number of devices fabricated) was extremely low (several %). Several years later, we have succeeded in significantly decreasing the base line noise by using a salt-bridge-type Ag/AgCl electrode and successfully demonstrated the application of an incubation type planar patch clamp to ligand gated ion-channel biosensors and light gated ion-channel biosensors using HEK293 cells expressed with ChRWR. Furthermore, a spontaneous ion-channel current from a neuronal network was successfully observed by using a planar patch clamp chip, on which the neuronal network was occasionally formed with a soma of a neuron on a micro through-hole. Although the neuronal network was not controlled, this success shows the high potential of realizing a high throughput screening device on the basis of channel current measurements, which contain the most important information on network conditions.

Upscaling and Automation of Electrophysiology: Toward High Throughput Screening in Ion Channel Drug Discovery

2003 ◽  
Vol 9 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Margit Asmild ◽  
Nicholas Oswald ◽  
Karen M. Krzywkowski ◽  
Søren Friis ◽  
Rasmus B. Jacobsen ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Ion Channel ◽  
High Throughput ◽  
High Throughput Screening

Application of High-throughput Screening Techniques to Drug Discovery

2000 ◽  
pp. 83-133 ◽  
Author(s):  
Brian Cox ◽  
Andy T. Merritt ◽  
Alastair Binnie ◽  
Mary C. Donnelly ◽  
Tom H. Mander ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
High Throughput Screening ◽  
Screening Techniques
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