Human neonatal and infant airway epithelial biology: The new frontier for developmental immunology

Species-Specific Differences in Mouse and Human Airway Epithelial Biology of Recombinant Adeno-Associated Virus Transduction

American Journal of Respiratory Cell and Molecular Biology ◽

10.1165/rcmb.2005-0189oc ◽

2006 ◽

Vol 34 (1) ◽

pp. 56-64 ◽

Cited By ~ 35

Author(s):

Xiaoming Liu ◽

Ziying Yan ◽

Meihui Luo ◽

John F. Engelhardt

Keyword(s):

Airway Epithelial ◽

Adeno Associated Virus ◽

Human Airway ◽

Species Specific ◽

Epithelial Biology

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Flow cytometric analysis and purification of airway epithelial cell subsets

10.1101/2020.04.20.051383 ◽

2020 ◽

Author(s):

Luke R. Bonser ◽

Kyung Duk Koh ◽

Kristina Johansson ◽

Semil P. Choksi ◽

Dan Cheng ◽

...

Keyword(s):

Epithelial Cell ◽

Flow Cytometric Analysis ◽

Cell Subset ◽

Airway Epithelial Cells ◽

Secretory Cells ◽

Airway Epithelial ◽

Specific Expression ◽

Flow Cytometric ◽

Micro Rnas ◽

Epithelial Biology

AbstractThe human airway epithelium is essential in homeostasis, and epithelial dysfunction contributes to chronic airway disease. Development of flow cytometric methods to characterize subsets of airway epithelial cells will enable further dissection of airway epithelial biology. Leveraging single cell RNA-sequencing (scRNA-seq) data in combination with known cell type-specific markers, we developed panels of antibodies to characterize and isolate the major airway epithelial subsets (basal, ciliated, and secretory cells) from human bronchial epithelial cell cultures. We also identified molecularly distinct subpopulations of secretory cells and demonstrated cell subset-specific expression of low abundance transcripts and micro-RNAs that are challenging to analyze with current scRNA-seq methods. These new tools will be valuable for analyzing and separating airway epithelial subsets and interrogating airway epithelial biology.

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New Ultrastructural Observations on Injury and Regeneration of Cilia in Mammalian Conducting Airway Epithelium

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099544 ◽

1981 ◽

Vol 39 ◽

pp. 624-625

Author(s):

J.L. Carson ◽

A.M. Collier

Keyword(s):

Respiratory Tract ◽

Airway Epithelium ◽

Defense Mechanisms ◽

Normal Growth ◽

Fetal Lung ◽

Secretory Cells ◽

Airway Epithelial ◽

Ciliated Cells ◽

Conducting Airway ◽

Conducting Airways

The ciliated cells lining the conducting airways of mammals are integral to the defense mechanisms of the respiratory tract, functioning in coordination with secretory cells in the removal of inhaled and cellular debris. The effects of various infectious and toxic agents on the structure and function of airway epithelial cell cilia have been studied in our laboratory, both of which have been shown to affect ciliary ultrastructure.These observations have led to questions about ciliary regeneration as well as the possible induction of ciliogenesis in response to cellular injury. Classical models of ciliogenesis in the conducting airway epithelium of the mammalian respiratory tract have been based primarily on observations of the developing fetal lung. These observations provide a plausible explanation for the embryological generation of ciliary beds lining the conducting airways but do little to account for subsequent differentiation of ciliated cells and ciliogenesis during normal growth and development.

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