scholarly journals Antibody repertoire analysis of mouse immunization protocols using microfluidics and molecular genomics

mAbs ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 870-883 ◽  
Author(s):  
Michael A. Asensio ◽  
Yoong Wearn Lim ◽  
Nicholas Wayham ◽  
Kacy Stadtmiller ◽  
Robert C. Edgar ◽  
...  
Keyword(s):  
Antibody Repertoire ◽  
Repertoire Analysis ◽  
Mouse Immunization

scholarly journals Intra-seasonal antibody repertoire analysis of a subject immunized with an MF59®-adjuvanted pandemic 2009 H1N1 vaccine

Vaccine ◽  
2018 ◽  
Vol 36 (35) ◽  
pp. 5325-5332 ◽  
Author(s):  
Jack Ferdman ◽  
Giuseppe Palladino ◽  
Hua-Xin Liao ◽  
M. Anthony Moody ◽  
Thomas B. Kepler ◽  
...  
Keyword(s):  
Antibody Repertoire ◽  
H1n1 Vaccine ◽  
Repertoire Analysis ◽  
2009 H1n1

scholarly journals Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Bo Wang ◽  
Christien A. Kluwe ◽  
Oana I. Lungu ◽  
Brandon J. DeKosky ◽  
Scott A. Kerr ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Ebola Virus ◽  
Sequence Divergence ◽  
West African ◽  
Antibody Repertoire ◽  
Efficient Technology ◽  
Mouse Immunization ◽  
Selection For ◽  
Reston Virus ◽  
Antibody Secreting Cells

Abstract The ongoing evolution of Ebolaviruses poses significant challenges to the development of immunodiagnostics for detecting emergent viral variants. There is a critical need for the discovery of monoclonal antibodies with distinct affinities and specificities for different Ebolaviruses. We developed an efficient technology for the rapid discovery of a plethora of antigen-specific monoclonal antibodies from immunized animals by mining the VH:VL paired antibody repertoire encoded by highly expanded B cells in the draining popliteal lymph node (PLN). This approach requires neither screening nor selection for antigen-binding. Specifically we show that mouse immunization with Ebola VLPs gives rise to a highly polarized antibody repertoire in CD138+ antibody-secreting cells within the PLN. All highly expanded antibody clones (7/7 distinct clones/animal) were expressed recombinantly and shown to recognize the VLPs used for immunization. Using this approach we obtained diverse panels of antibodies including: (i) antibodies with high affinity towards GP; (ii) antibodies which bound Ebola VLP Kissidougou-C15, the strain circulating in the recent West African outbreak; (iii) non-GP binding antibodies that recognize wild type Sudan or Bundibugyo viruses that have 39% and 37% sequence divergence from Ebola virus, respectively and (iv) antibodies to the Reston virus GP for which no antibodies have been reported.

scholarly journals Antibody Repertoire Analysis of Tumor-Infiltrating B Cells Reveals Distinct Signatures and Distributions Across Tissues

2021 ◽  
Vol 12 ◽  
Author(s):  
Ligal Aizik ◽  
Yael Dror ◽  
David Taussig ◽  
Adi Barzel ◽  
Yaron Carmi ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
High Throughput Sequencing ◽  
Triple Negative ◽  
Somatic Hypermutation ◽  
Cell Receptor ◽  
Antibody Repertoire ◽  
Class Switch ◽  
Repertoire Analysis

The role of B cells in the tumor microenvironment (TME) has largely been under investigated, and data regarding the antibody repertoire encoded by B cells in the TME and the adjacent lymphoid organs are scarce. Here, we utilized B cell receptor high-throughput sequencing (BCR-Seq) to profile the antibody repertoire signature of tumor-infiltrating lymphocyte B cells (TIL−Bs) in comparison to B cells from three anatomic compartments in a mouse model of triple-negative breast cancer. We found that TIL-Bs exhibit distinct antibody repertoire measures, including high clonal polarization and elevated somatic hypermutation rates, suggesting a local antigen-driven B-cell response. Importantly, TIL-Bs were highly mutated but non-class switched, suggesting that class-switch recombination may be inhibited in the TME. Tracing the distribution of TIL-B clones across various compartments indicated that they migrate to and from the TME. The data thus suggests that antibody repertoire signatures can serve as indicators for identifying tumor-reactive B cells.

scholarly journals Antibody repertoire analysis in polygenic autoimmune diseases

Immunology ◽  
2018 ◽  
Vol 155 (1) ◽  
pp. 3-17 ◽  
Author(s):  
Rachael J. M. Bashford-Rogers ◽  
Kenneth G. C. Smith ◽  
David C. Thomas
Keyword(s):  
Autoimmune Diseases ◽  
Antibody Repertoire ◽  
Repertoire Analysis

scholarly journals Antibody repertoire analysis of tumor-infiltrating B cells reveals distinct signatures and distributions across tissues

2021 ◽  
Author(s):  
Ligal Aizik ◽  
Yael Dror ◽  
David Taussig ◽  
Adi Barzel ◽  
Yaron Carmi ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
High Throughput Sequencing ◽  
Triple Negative ◽  
Somatic Hypermutation ◽  
Cell Receptor ◽  
Antibody Repertoire ◽  
Class Switch ◽  
Repertoire Analysis

The role of B cells in the tumor microenvironment (TME) has largely been under-investigated, and data regarding the antibody repertoire encoded by B cells in the TME and the adjacent lymphoid organs are scarce. Here, we utilized B cell receptor high-throughput sequencing (BCR-Seq) to profile the antibody repertoire signature of tumor-infiltrating lymphocyte B cells (TIL Bs) in comparison to B cells from three anatomic compartments in a mouse model of triple-negative breast cancer. We found that TIL-Bs exhibit distinct antibody repertoire measures, including high clonal polarization and elevated somatic hypermutation rates, suggesting a local antigen-driven B-cell response. Importantly, TIL-Bs were highly mutated but non-class switched, suggesting that class-switch recombination may be inhibited in the TME. Tracing the distribution of TIL-B clones across various compartments indicated that they migrate to and from the TME. The data thus suggests that antibody repertoire signatures can serve as indicators for identifying tumor-reactive B cells.

scholarly journals Distinct repertoire expansion patterns during affinity maturation of antigen responding camelid-derived single-domain VHH antibody

2021 ◽  
Author(s):  
Tomonari Matsuda ◽  
Yoko Akazawa-Ogawa ◽  
Lilian-Kaede Komaba ◽  
Norihiko Kiyose ◽  
Nobuo Miyazaki ◽  
...  
Keyword(s):  
Affinity Maturation ◽  
Physical Characteristics ◽  
Single Domain ◽  
Antibody Repertoire ◽  
Single Domain Antibody ◽  
Phylogenetic Cluster ◽  
Domain Antibody ◽  
Repertoire Analysis ◽  
Molecular Phylogenetic ◽  
Conventional Antibody

It is difficult to link antibody repertoire expansion with changes in the physical characteristics of antigen-responding antibodies as correct light-chain and heavy-chain matching and conventional antibody production are laborious. Utilization of single-domain antibody solved these problems. A blood of immunized alpaca was collected weekly for three months for antibody repertoire analysis. The sequences processed to cluster and recombinant antibodies were generated to determine whether the clusters respond to antigens. The repertoire expansion in most of the antigen-responding clusters exhibited distinct patterns as compared to that of antigen irrelevant clusters. In addition, the sequences at the tips and root in the molecular phylogenetic cluster tree have strong and weak antigen affinity, respectively. These features may be utilized to predict clusters of antigen-binding antibodies and physical characteristics of antibodies.

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