scholarly journals RETRACTED ARTICLE: DNA barcoding identification of Pseudococcidae (Hemiptera: Coccoidea) using the mitochondrial COI gene

2018 ◽  
Vol 3 (1) ◽  
pp. 419-423 ◽  
Author(s):  
Sizhu Zheng ◽  
Yang Li ◽  
Xiaojun Yang ◽  
Jingyun Chen ◽  
Jing Hua ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Retracted Article

DNA barcoding and phylogenetic analysis of bagrid catfish in China based on mitochondrial COI gene

2020 ◽  
Vol 31 (2) ◽  
pp. 73-80
Author(s):  
Rui Zou ◽  
Cong Liang ◽  
Mengmeng Dai ◽  
Xiaodong Wang ◽  
Xiuyue Zhang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Barcoding ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

DNA barcodes for Cladocera and Copepoda from Mexico and Guatemala, highlights and new discoveries

Zootaxa ◽  
2008 ◽  
Vol 1839 (1) ◽  
pp. 1 ◽  
Author(s):  
MANUEL ELÍAS-GUTIÉRREZ ◽  
FERNANDO MARTÍNEZ JERÓNIMO ◽  
NATALIA V. IVANOVA ◽  
MARTHA VALDEZ-MORENO ◽  
PAUL D. N. HEBERT
Keyword(s):  
Dna Barcoding ◽  
Classification Systems ◽  
Coi Gene ◽  
Aquatic Environments ◽  
Dna Barcodes ◽  
Chydorus Sphaericus ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Current Classification ◽  
Excellent Tool

DNA barcoding, based on sequence diversity in the mitochondrial COI gene, has proven an excellent tool for identifying species in many animal groups. Here, we report the first barcode studies for freshwater zooplankton from Mexico and Guatemala and discuss the taxonomic and biological implications of this work. Our studies examined 61 species of Cladocera and 21 of Copepoda, about 40% of the known fauna in this region. Sequence divergences among conspecific individuals of cladocerans and copepods averaged 0.82% and 0.79%, respectively, while sequence divergences among congeneric taxa were on average 15-20 times as high. Barcodes were successful in discriminating all species in our study, but sequences for Mexican Daphnia exilis overlapped with those of D. spinulata from Argentina. Our barcode data revealed evidence of many species overlooked by current classification systems —for example, based on COI genotypes the Diapahanosoma birgei group appears to include 5 species, while Ceriodaphnia cf. rigaudi, Moina cf. micrura, Mastigodiaptomus albuquerquensis and Mastigodiaptomus reidae all include 2–3 taxa. The barcode results support recent taxonomic revisions, such as recognition of the genus Leberis, and the presence of several species in the D. birgei and Chydorus sphaericus complexes. The present results indicate that DNA barcoding will provide powerful new insights into both the incidence of cryptic species and a better understanding of zooplankton distributions, aiding evaluation of the factors influencing competitive outcomes, and the colonization of aquatic environments.

Discovery of the genus Claraeola Aczél in Iran with the description of two new species (Diptera: Pipunculidae)

Zootaxa ◽  
2017 ◽  
Vol 4227 (4) ◽  
pp. 563 ◽  
Author(s):  
BEHNAM MOTAMEDINIA ◽  
CHRISTIAN KEHLMAIER ◽  
AZIZOLLAH MOKHTARI ◽  
EHSAN RAKHSHANI ◽  
EBRAHIM GILASIAN
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Identification Key ◽  
Coi Gene ◽  
Palaearctic Species ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Two New Species ◽  
Western Palaearctic ◽  
First Time

The genus Claraeola Aczél is recorded from Iran for the first time. Two new species, Claraeola parnianae Motamedinia & Kehlmaier sp. nov. and Claraeola khorshidae Motamedinia & Kehlmaier sp. nov., are described and illustrated. An updated identification key to the Western Palaearctic species of the genus Claraeola is provided. Both species were characterized morphologically and by DNA barcoding of the mitochondrial COI gene. 

Description of a new species of the genus Maua Distant (Hemiptera, Cicadidae) from China

Zootaxa ◽  
2021 ◽  
Vol 4949 (3) ◽  
pp. 582-588
Author(s):  
JIALI WANG ◽  
JOANNES P. DUFFELS ◽  
CONG WEI
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Intraspecific Variation ◽  
Male Genitalia ◽  
Morphological Characters ◽  
Coi Gene ◽  
Morphological Observation ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
A New Species

A new species, Maua squeala sp. nov., is described from China. This species is similar to M. affinis Distant, 1905 and M. palawanensis Duffels, 2009, but can be distinguished by the shorter and more slender body of the new species, the lateral fasciae on the mesonotum and the shape of the male genitalia. The intraspecific variation of this species is discussed based on morphological observation combined with sequences of partial mitochondrial COI gene (DNA barcoding) of individuals exhibiting different morphological characters. 

scholarly journals The genus Dasydorylas Skevington in Iran, with the description of two new species (Diptera: Pipunculidae)

2017 ◽  
Author(s):  
Behnam Motamedinia ◽  
Christian Kehlmaier ◽  
Azizollah Mokhtari ◽  
Ehsan Rakhshani ◽  
Ebrahim Gilasian
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Identification Key ◽  
Coi Gene ◽  
Palaearctic Species ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Two New Species ◽  
Western Palaearctic

The genus Dasydorylas Skevington, 2001 is recorded from two provinces in Iran (Sistan-o Baluchestan and Kermanshah Provinces). Dasydorylas derafshani sp. nov. and D. zardouei sp. nov. are illustrated and characterized morphologically and by DNA barcoding of the mitochondrial COI gene. Eudorylas antennalis Kapoor, Grewal & Sharma, 1987 is transferred to Dasydorlyas (comb. nov.). An existing identification key to the males of the western Palaearctic species of Dasydorylas is complemented to include the newly described species.

scholarly journals Italian Odonates in the Pandora’s Box: A Comprehensive DNA Barcoding Inventory Shows Taxonomic Warnings at the Holarctic Scale

2020 ◽  
Author(s):  
Andrea Galimberti ◽  
Giacomo Assandri ◽  
Davide Maggioni ◽  
Fausto Ramazzotti ◽  
Daniele Baroni ◽  
...  
Keyword(s):  
Cryptic Species ◽  
Dna Barcoding ◽  
Genetic Divergence ◽  
Species Delimitation ◽  
Coi Gene ◽  
Dna Barcodes ◽  
Mitochondrial Coi ◽  
Haplotype Networks ◽  
Mitochondrial Coi Gene

AbstractThe Odonata are considered among the most endangered freshwater faunal taxa. Their DNA-based monitoring relies on validated reference datasets that are often lacking or do not cover important biogeographical centres of diversification. This study presents the results of a DNA barcoding campaign on Odonata, based on the standard 658 bp 5’ end region of the mitochondrial COI gene, involving the collection of 812 specimens (409 of which barcoded) from peninsular Italy and its main islands (328 localities), belonging to all the 88 species (31 Zygoptera and 57 Anisoptera) known from the country. Additional BOLD and GenBank data from Holarctic samples expanded the dataset to 1294 DNA barcodes. A multi-approach species delimitation analysis involving two distance (OT and ABGD) and four tree-based (PTP, MPTP, GMYC, bGMYC) methods were used to explore these data. Of the 88 investigated morphospecies, 75 (85%) unequivocally corresponded to distinct Molecular Operational Units, whereas the remaining ones were classified as ‘warnings’ (i.e., showing a mismatch between morphospecies assignment and DNA-based species delimitation). These results are in contrast with other DNA barcoding studies on Odonata showing up to 95% of identification success. The species causing warnings were grouped in three categories depending on if they showed low, high, or mixed genetic divergence patterns. The analysis of haplotype networks revealed unexpected intraspecific complexity at the Italian, Palearctic, and Holarctic scale, possibly indicating the occurrence of cryptic species. Overall, this study provides new insights into the taxonomy of odonates and a valuable basis for future DNA and eDNA-based monitoring studies.

Morphological description and DNA barcoding of Hydrobaenus golovinensis sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Russian Far East

Zootaxa ◽  
2017 ◽  
Vol 4286 (2) ◽  
pp. 277
Author(s):  
EUGENYI A. MAKARCHENKO ◽  
MARINA A. MAKARCHENKO ◽  
ALEXANDER A. SEMENCHENKO ◽  
OLEG A. VELIAEV
Keyword(s):  
Dna Barcoding ◽  
Russian Far East ◽  
Far East ◽  
Instar Larva ◽  
Genetic Distances ◽  
Coi Gene ◽  
Morphological Description ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
The Russian Far East

Illustrated descriptions of adult male, pupa and fourth instar larva, as well as DNA barcoding results of Hydrobaenus golovinensis sp. nov. in comparison with closely related species H. majus Makarchenko et Makarchenko and H. sikhotealinensis Makarchenko et Makarchenko from the Russian Far East are provided. Partial mitochondrial COI gene (DNA barcoding) of the new species has been sequenced and uploaded to GenBank. Comparisons with corresponding regions of COI between H. golovinensis and other species in the genus produce K2P genetic distances of 10.3–14.3%, the values well above those associated with intraspecific variation. In contrast, genetic distances among 18 specimens are all within the range of 0–3.5%. The ML tree is also constructed using DNA barcodes obtained in the present study and those of other species of Hydrobaenus Fries from GenBank. 

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