DNA barcodes for Cladocera and Copepoda from Mexico and Guatemala, highlights and new discoveries

Zootaxa ◽  
2008 ◽  
Vol 1839 (1) ◽  
pp. 1 ◽  
Author(s):  
MANUEL ELÍAS-GUTIÉRREZ ◽  
FERNANDO MARTÍNEZ JERÓNIMO ◽  
NATALIA V. IVANOVA ◽  
MARTHA VALDEZ-MORENO ◽  
PAUL D. N. HEBERT
Keyword(s):  
Dna Barcoding ◽  
Classification Systems ◽  
Coi Gene ◽  
Aquatic Environments ◽  
Dna Barcodes ◽  
Chydorus Sphaericus ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Current Classification ◽  
Excellent Tool

DNA barcoding, based on sequence diversity in the mitochondrial COI gene, has proven an excellent tool for identifying species in many animal groups. Here, we report the first barcode studies for freshwater zooplankton from Mexico and Guatemala and discuss the taxonomic and biological implications of this work. Our studies examined 61 species of Cladocera and 21 of Copepoda, about 40% of the known fauna in this region. Sequence divergences among conspecific individuals of cladocerans and copepods averaged 0.82% and 0.79%, respectively, while sequence divergences among congeneric taxa were on average 15-20 times as high. Barcodes were successful in discriminating all species in our study, but sequences for Mexican Daphnia exilis overlapped with those of D. spinulata from Argentina. Our barcode data revealed evidence of many species overlooked by current classification systems —for example, based on COI genotypes the Diapahanosoma birgei group appears to include 5 species, while Ceriodaphnia cf. rigaudi, Moina cf. micrura, Mastigodiaptomus albuquerquensis and Mastigodiaptomus reidae all include 2–3 taxa. The barcode results support recent taxonomic revisions, such as recognition of the genus Leberis, and the presence of several species in the D. birgei and Chydorus sphaericus complexes. The present results indicate that DNA barcoding will provide powerful new insights into both the incidence of cryptic species and a better understanding of zooplankton distributions, aiding evaluation of the factors influencing competitive outcomes, and the colonization of aquatic environments.

scholarly journals Italian Odonates in the Pandora’s Box: A Comprehensive DNA Barcoding Inventory Shows Taxonomic Warnings at the Holarctic Scale

2020 ◽  
Author(s):  
Andrea Galimberti ◽  
Giacomo Assandri ◽  
Davide Maggioni ◽  
Fausto Ramazzotti ◽  
Daniele Baroni ◽  
...  
Keyword(s):  
Cryptic Species ◽  
Dna Barcoding ◽  
Genetic Divergence ◽  
Species Delimitation ◽  
Coi Gene ◽  
Dna Barcodes ◽  
Mitochondrial Coi ◽  
Haplotype Networks ◽  
Mitochondrial Coi Gene

AbstractThe Odonata are considered among the most endangered freshwater faunal taxa. Their DNA-based monitoring relies on validated reference datasets that are often lacking or do not cover important biogeographical centres of diversification. This study presents the results of a DNA barcoding campaign on Odonata, based on the standard 658 bp 5’ end region of the mitochondrial COI gene, involving the collection of 812 specimens (409 of which barcoded) from peninsular Italy and its main islands (328 localities), belonging to all the 88 species (31 Zygoptera and 57 Anisoptera) known from the country. Additional BOLD and GenBank data from Holarctic samples expanded the dataset to 1294 DNA barcodes. A multi-approach species delimitation analysis involving two distance (OT and ABGD) and four tree-based (PTP, MPTP, GMYC, bGMYC) methods were used to explore these data. Of the 88 investigated morphospecies, 75 (85%) unequivocally corresponded to distinct Molecular Operational Units, whereas the remaining ones were classified as ‘warnings’ (i.e., showing a mismatch between morphospecies assignment and DNA-based species delimitation). These results are in contrast with other DNA barcoding studies on Odonata showing up to 95% of identification success. The species causing warnings were grouped in three categories depending on if they showed low, high, or mixed genetic divergence patterns. The analysis of haplotype networks revealed unexpected intraspecific complexity at the Italian, Palearctic, and Holarctic scale, possibly indicating the occurrence of cryptic species. Overall, this study provides new insights into the taxonomy of odonates and a valuable basis for future DNA and eDNA-based monitoring studies.

scholarly journals DNA BARCODING APPLICATION OF MITOCHONDRIAL COI GENE TO IDENTIFY SOME FISH SPECIES OF FAMILY GOBIIDAE IN VIETNAM

2019 ◽  
Vol 18 (4) ◽  
pp. 443-451
Author(s):  
Nguyen Manh Linh ◽  
Pham The Thu ◽  
Nguyen Van Quan ◽  
Pham Van Chien ◽  
Dao Huong Ly ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Coi Gene ◽  
Dna Barcodes ◽  
North Central ◽  
Coastal Fish ◽  
Mitochondrial Coi ◽  
Fisheries Resources ◽  
Mitochondrial Coi Gene ◽  
The Family ◽  
Mitochondrial Cytochrome Oxidase

The family Gobiidae is a relatively high abundance family of coastal fish with about 2000 species in 210 genera described. In Vietnam, the study on Gobiidae is very complex due to the large number of species, small size and high morphological variation which makes difficulties in identification and classification. In this study, the DNA barcoding technique has been used through mitochondrial cytochrome oxidase I (COI) gene to classify 30 specimens of Gobiidae from three coastal areas (North, Central and South) in Vietnam to evaluate the effectiveness compared to the morphological classification method. Results showed that 26 species belonging to 21 genera were determined by the COI barcode while 19 species (17 genera) were determined when using morphological method. Mahidolia mystacina was new recorded in Vietnam. The DNA barcodes of COI gene developed in this study could be useful for estimating phylogenetic diversity as well as other studies of gobiids in terms of conservation, management and utilization of fisheries resources in Vietnam. In addition, the results showed the high potentiality in using COI barcode to identify marine fish.

DNA barcoding and phylogenetic analysis of bagrid catfish in China based on mitochondrial COI gene

2020 ◽  
Vol 31 (2) ◽  
pp. 73-80
Author(s):  
Rui Zou ◽  
Cong Liang ◽  
Mengmeng Dai ◽  
Xiaodong Wang ◽  
Xiuyue Zhang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Barcoding ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

scholarly journals RETRACTED ARTICLE: DNA barcoding identification of Pseudococcidae (Hemiptera: Coccoidea) using the mitochondrial COI gene

2018 ◽  
Vol 3 (1) ◽  
pp. 419-423 ◽  
Author(s):  
Sizhu Zheng ◽  
Yang Li ◽  
Xiaojun Yang ◽  
Jingyun Chen ◽  
Jing Hua ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Retracted Article

scholarly journals Pseudanthias hangapiko, a new anthiadine serranid (Teleostei, Serranidae, Anthiadinae) from Rapa Nui (Easter Island)

ZooKeys ◽  
2021 ◽  
Vol 1054 ◽  
pp. 1-13
Author(s):  
Bart Shepherd ◽  
Hudson T. Pinheiro ◽  
Tyler A. Y. Phelps ◽  
Alejandro Pérez-Matus ◽  
Luiz A. Rocha
Keyword(s):  
Easter Island ◽  
Coi Gene ◽  
Dna Barcodes ◽  
Rapa Nui ◽  
Body Depth ◽  
Mitochondrial Coi ◽  
Dorsal Fin ◽  
Single Location ◽  
Mitochondrial Coi Gene ◽  
Fin Rays

Pseudanthias hangapikosp. nov. (Teleostei, Serranidae, Anthiadinae) is herein described from three specimens collected from a depth of 83 m in a mesophotic coral ecosystem off Hanga Piko, Rapa Nui (Easter Island), Chile. Pseudanthias hangapikosp. nov. can be distinguished from its congeners in live coloration and by the following combination of characters: dorsal-fin rays X, 17; anal-fin rays III, 8; pectoral-fin rays 16 (left side of one specimen 17); vertebrae 10+16; scales relatively large, two scales above lateral-line to base of fifth dorsal spine, and 16–17 circumpeduncular scales; gill rakers 11+23; and a slender body, with greatest body depth 3.6 (3.4–3.8) in SL. The most similar DNA barcodes (mitochondrial COI gene) are from Pseudanthias ventralis Randall, 1979 and Pseudanthias hawaiiensis Randall, 1979, with 16.8% and 17.0% uncorrected divergence, respectively. This fish is one of four new species that were documented from a pair of technical dives at a single location in Rapa Nui, emphasizing the high number of undescribed species likely still unknown in mesophotic coral ecosystems, especially in geographically remote locations. Pseudanthias hangapikosp. nov. adds to the Rapa Nui ichthyofauna, which hosts the second-highest level of endemism in both shallow and deep-water fishes.

Discovery of the genus Claraeola Aczél in Iran with the description of two new species (Diptera: Pipunculidae)

Zootaxa ◽  
2017 ◽  
Vol 4227 (4) ◽  
pp. 563 ◽  
Author(s):  
BEHNAM MOTAMEDINIA ◽  
CHRISTIAN KEHLMAIER ◽  
AZIZOLLAH MOKHTARI ◽  
EHSAN RAKHSHANI ◽  
EBRAHIM GILASIAN
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Identification Key ◽  
Coi Gene ◽  
Palaearctic Species ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Two New Species ◽  
Western Palaearctic ◽  
First Time

The genus Claraeola Aczél is recorded from Iran for the first time. Two new species, Claraeola parnianae Motamedinia & Kehlmaier sp. nov. and Claraeola khorshidae Motamedinia & Kehlmaier sp. nov., are described and illustrated. An updated identification key to the Western Palaearctic species of the genus Claraeola is provided. Both species were characterized morphologically and by DNA barcoding of the mitochondrial COI gene. 

Description of a new species of the genus Maua Distant (Hemiptera, Cicadidae) from China

Zootaxa ◽  
2021 ◽  
Vol 4949 (3) ◽  
pp. 582-588
Author(s):  
JIALI WANG ◽  
JOANNES P. DUFFELS ◽  
CONG WEI
Keyword(s):  
New Species ◽  
Dna Barcoding ◽  
Intraspecific Variation ◽  
Male Genitalia ◽  
Morphological Characters ◽  
Coi Gene ◽  
Morphological Observation ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
A New Species

A new species, Maua squeala sp. nov., is described from China. This species is similar to M. affinis Distant, 1905 and M. palawanensis Duffels, 2009, but can be distinguished by the shorter and more slender body of the new species, the lateral fasciae on the mesonotum and the shape of the male genitalia. The intraspecific variation of this species is discussed based on morphological observation combined with sequences of partial mitochondrial COI gene (DNA barcoding) of individuals exhibiting different morphological characters. 

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